Other Publications (1)
Articles by Mia Klekos in JoVE
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains Kathryn E. Neville1, Timothy L. Bosse1, Mia Klekos1, John F. Mills1, Marla Tipping1 1Department of Biology, Providence College We present a protocol for measuring oxygen consumption and extracellular acidification in Drosophila melanogaster larval and adult brains. A metabolic analyzer is utilized with an adapted and optimized protocol. Micro-tissue restraints are a critical component of this protocol and were designed and created specifically for their use in this analysis.
Other articles by Mia Klekos on PubMed
A Novel Ex Vivo Method for Measuring Whole Brain Metabolism in Model Systems Journal of Neuroscience Methods. Feb, 2018 | Pubmed ID: 29287743 Many neuronal and glial diseases have been associated with changes in metabolism. Therefore, metabolic reprogramming has become an important area of research to better understand disease at the cellular level, as well as to identify targets for treatment. Model systems are ideal for interrogating metabolic questions in a tissue dependent context. However, while new tools have been developed to study metabolism in cultured cells there has been less progress towards studies in vivo and ex vivo.