In JoVE (1)
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Articles by Michael J. Gerger in JoVE
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System Michael K. Conway1, Michael J. Gerger1, Erin E. Balay1, Rachel O'Connell1, Seth Hanson2, Neil J. Daily1, Tetsuro Wakatsuki1 1InvivoSciences, Inc., 2Gilson, Inc. The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.