Articles by Michio Kimura in JoVE
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes Hiroyuki Yamamoto1, Yoshikazu Sawaguchi2, Michio Kimura1 1Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics, Nihon Pharmaceutical University Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.
Other articles by Michio Kimura on PubMed
Identification of Protease Specificity Using Biotin-Labeled Substrates The Open Biochemistry Journal. | Pubmed ID: 28567123 Proteolysis constitutes a major post-translational modification. For example, proteases regulate the activation or inactivation of various proteins, such as enzymes, growth factors, and peptide hormones. Proteases have substrate specificity, and protease expression regulates the specific and regional activation or inactivation of several functional proteins.