In JoVE (1)
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Articles by Miguel I. Uyaguari-Diaz in JoVE
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples Miguel I. Uyaguari-Diaz1, Jared R. Slobodan2, Matthew J. Nesbitt2, Matthew A. Croxen3, Judith Isaac-Renton1,3, Natalie A. Prystajecky1,3, Patrick Tang1,3 1Department of Pathology and Laboratory Medicine, Faculty of Medicine, The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.