Other Publications (1)
Articles by Miguel Martí in JoVE
Antimicrobial Characterization of Advanced Materials for Bioengineering Applications Miguel Martí*1, Belén Frígols*1, Angel Serrano-Aroca1 1Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir We present a protocol for the antimicrobial characterization of advanced materials. Here, the antimicrobial activity on material surfaces is measured by two methods that complement each other: one is based on the agar disk diffusion test, and the other is a standard procedure based on the ISO 22196:2007 norm.
Other articles by Miguel Martí on PubMed
Sensory Deprivation in Staphylococcus Aureus Nature Communications. 02, 2018 | Pubmed ID: 29410457 Bacteria use two-component systems (TCSs) to sense and respond to environmental changes. The core genome of the major human pathogen Staphylococcus aureus encodes 16 TCSs, one of which (WalRK) is essential. Here we show that S. aureus can be deprived of its complete sensorial TCS network and still survive under growth arrest conditions similarly to wild-type bacteria. Under replicating conditions, however, the WalRK system is necessary and sufficient to maintain bacterial growth, indicating that sensing through TCSs is mostly dispensable for living under constant environmental conditions. Characterization of S. aureus derivatives containing individual TCSs reveals that each TCS appears to be autonomous and self-sufficient to sense and respond to specific environmental cues, although some level of cross-regulation between non-cognate sensor-response regulator pairs occurs in vivo. This organization, if confirmed in other bacterial species, may provide a general evolutionarily mechanism for flexible bacterial adaptation to life in new niches.