In JoVE (1)
Other Publications (1)
Articles by Ming H. Lye in JoVE
عضوي النمط من الثقافة الشبكية أرنب الكبار Ming H. Lye1, Tatjana C. Jakobs1, Richard H. Masland1, Amane Koizumi1 1Havard Medical School, MGH - Massachusetts General Hospital هذا المقال يوضح تشريح وحضانة للشبكية الأرانب والجسيمات بوساطة نقل الجينات من البلازميدات GFP ترميز أو مجموعة متنوعة من العلامات التحت خلوية إلى خلايا الشبكية العقدة.
Other articles by Ming H. Lye on PubMed
Eosinophils Adhere to Vascular Cell Adhesion Molecule-1 Via Podosomes American Journal of Respiratory Cell and Molecular Biology. Oct, 2004 | Pubmed ID: 15220135 Vascular cell adhesion molecule (VCAM)-1 supports specific eosinophil adhesion via alpha4beta1 integrin. We tested the hypothesis that adhesive contacts formed by eosinophils on VCAM-1 are different from focal adhesions formed by adherent fibroblasts. Eosinophils adherent on VCAM-1 formed punctate adhesions that fit the criteria for podosomes, highly dynamic structures found in adherent transformed fibroblasts, osteoclasts, and macrophages. The structures contained beta1 integrin subunit, phosphotyrosine-containing proteins, punctate filamentous actin, and gelsolin, a podosome marker. In contrast, nontransformed fibroblasts on VCAM-1 formed peripheral focal adhesions that were positive for alpha4, beta1, phosphotyrosine, vinculin, talin, and paxillin; negative for gelsolin; and associated with microfilaments. Phorbol myristate acetate or tumor necrosis factor-alpha and interleukin-5 stimulated podosome formation in adherent eosinophils. Because podosomes in tumor cells are associated with extracellular matrix degradation, we analyzed the VCAM-1 layer. VCAM-1 was lost under adherent eosinophils but not under adherent fibroblasts. This loss was inhibited by the metalloproteinase inhibitor ortho-phenanthroline and correlated with expression and podosome localization of a membrane-tethered metalloproteinase, a disintegrin and metalloproteinase domain 8. Podosome-mediated VCAM-1 clearance may be a mechanism to regulate eosinophil arrest and extravasation in allergic conditions such as asthma.