In JoVE (1)
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Articles by Mirjam Tyka in JoVE
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization Christian Erbel1, Gregor Rupp1, Christian M. Helmes1, Mirjam Tyka1, Fabian Linden1, Andreas O. Doesch1, Hugo A. Katus1, Christian A. Gleissner1 1Department of Cardiology, University of Heidelberg Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.