In JoVE (1)
Articles by Mitchell Bijnen in JoVE
Karakterisering van Immune cellen in menselijke adipeus weefsel met behulp van Stroom Cytometry Suzan Wetzels*1,2,3, Mitchell Bijnen*1,2, Erwin Wijnands2,4, Erik A.L. Biessen2,4, Casper G. Schalkwijk1,2, Kristiaan Wouters1,2 1Department of Internal Medicine, MUMC, 2CARIM, MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, 4Department of Pathology, MUMC Dit artikel beschrijft een methode voor het analyseren van immuun celinhoud van adipeus weefsel door isolatie van immuuncellen uit vetweefsel en latere analyse met behulp van stroom cytometry.
Other articles by Mitchell Bijnen on PubMed
Circulating Classical Monocytes Are Associated with CD11cmacrophages in Human Visceral Adipose Tissue Scientific Reports. | Pubmed ID: 28198418 Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting in metabolic dysfunction. Circulating immune cell patterns may reflect immune cell accumulation in expanding AT. However, data linking human leukocytes in blood and AT is lacking. We investigated whether blood immune cell populations are associated with their counterparts in subcutaneous (scAT) or visceral AT (vAT). Flow cytometry was performed on blood, scAT and vAT from 16 lean and 29 obese men. Circulating natural killer (NK)-cells, classical monocytes and nonclassical monocytes were higher in obese individuals. vAT, but not scAT, of obese individuals contained more inflammatory CD11c"M1" macrophages and NK cells compared to lean individuals. Blood classical monocytes were associated with CD11cmacrophages in vAT but not scAT. This association was unrelated to expression of the adhesion molecules CD11b and CD11c or of the chemokine receptor CX3CR1 on these monocytes. Other AT immune cells were not associated with their respective counterparts in blood. Finally, CD11cmacrophages and CD4T-cells in vAT were associated with their counterparts in scAT. In conclusion, blood classical monocytes reflect CD11cmacrophages in vAT.
Adipose Tissue Macrophages Induce Hepatic Neutrophil Recruitment and Macrophage Accumulation in Mice Gut. | Pubmed ID: 29074725 Obesity is a risk factor for non-alcoholic steatohepatitis (NASH). This risk has been attributed to visceral adipose tissue (vAT) expansion associated with increased proinflammatory mediators. Accumulation of CD11cproinflammatory adipose tissue macrophages (ATM) is an important driver of vAT inflammation. We investigated the role of ATMs in hepatic inflammation during NASH development.