Other Publications (1)
Articles by Muznah Khan in JoVE
Generering Primær fibroblastkulturer fra museøre og hale Væv Muznah Khan1, Stephan Gasser1,2 1Immunology Program, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore Vi beskriver en enkel og hurtig eksperimentelle procedure til generering af primære fibroblaster fra ørerne og haler af mus. Proceduren kræver ikke særlig dyretræningssystem og kan anvendes til generering af fibroblastkulturer fra ører opbevaret ved stuetemperatur i op til 10 dage.
Other articles by Muznah Khan on PubMed
The DNA Damage Response Induces Antigen Presenting Cell-like Functions in Fibroblasts European Journal of Immunology. Apr, 2014 | Pubmed ID: 24375454 The DNA damage response (DDR) alerts the immune system to the danger posed by DNA damage through the induction of damage-associated molecular pattern molecules, chemokines, and ligands for activating immune receptors such as lymphocyte function-associated antigen 1 (LFA-1), NKG2D, and DNAX accessory molecule 1 (DNAM-1). Here we provide evidence that OVA(257-264) -pulsed fibroblasts gain the ability to activate naïve OT-I CD8(+) T cells in response to DNA damage. The ability of fibroblasts to activate OT-I CD8(+) T cells depended on the upregulation of ICAM-1 on fibroblasts and DNAM-1 expression of CD8(+) T cells. OVA(257-264) -pulsed fibroblasts were able to induce a protective T-cell response against B16-OVA cells in a DDR-dependent manner. Hence, the DDR may alert the immune system to the presence of potentially dangerous cells by upregulating the expression of ligands that can induce the activation of innate and adaptive immune cells.