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Articles by Nader El Seblani in JoVE
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue Anika M.S. Hartz1, Julia A. Schulz2, Brent S. Sokola2, Stephanie E. Edelmann1, Andrew N. Shen1, Ralf G. Rempe2, Yu Zhong1, Nader El Seblani3, Bjoern Bauer2 1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences, University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 3Department of Neuroscience, University of Kentucky Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.