Articles by Niall J. Logan in JoVE
Evaluating Primary Blast Effects In Vitro Niall J. Logan1, Hari Arora1, Claire A. Higgins1 1Department of Bioengineering, Imperial College London Understanding how cells are modulated by exposure to shock waves can help identify the mechanisms behind injuries triggered from blast events. This protocol uses custom-built shock tube equipment to apply shock waves at a range of pressures to cell monolayers and to identify the subsequent effects on cell viability.
Other articles by Niall J. Logan on PubMed
Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles Experimental Dermatology. Jun, 2017 | Pubmed ID: 28418608 The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension. As such, to grow human dermal papilla cells in vitro, the papilla has to first be isolated via a micro-dissection approach from the follicle. In this article we describe the micro-dissection and culture methods, which we use within our laboratory, for the study of human dermal papilla cells.