In JoVE (1)
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Articles by Nicholas A. Suhar in JoVE
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D Bauer L. LeSavage*1, Nicholas A. Suhar*2, Christopher M. Madl1, Sarah C. Heilshorn2 1Department of Bioengineering, Stanford University, 2Department of Materials Science and Engineering, Stanford University Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.