Articles by Nico Grüner in JoVE
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques Nico Grüner1, Oumaima Stambouli1, R. Stefan Ross1 1Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital, University of Duisburg-Essen The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.
Other articles by Nico Grüner on PubMed
Composition of Humic Acid-degrading Estuarine and Marine Bacterial Communities FEMS Microbiology Ecology. Apr, 2012 | Pubmed ID: 22133061 We examined the bacterial decomposition of humic acids (HA) in two flow-through culture experiments, one inoculated by marine and one by estuarine bacterial communities. In both experiments, the cultures were fed with HA media of salinities of 28 and 14, close to their ambient and a distinctly different, foreign salinity. HA were decomposed to > 60% of the initial concentration within 70 days, and the foreign salinity yielded the highest decomposition. A detrended correspondence analysis of denaturing gradient gel electrophoresis (DGGE) banding patterns showed that during incubation, the bacterial community composition underwent distinct changes. A phylogenetic analysis of DGGE bands excised and bacteria isolated at the end on HA as the sole carbon source showed that Alphaproteobacteria and Gammaproteobacteria largely dominated the communities in the marine flow-through cultures, whereas Gammaproteobacteria, Actinobacteria and Alphaproteobacteria dominated the estuarine communities. Eleven of 13 isolates obtained from both experiments were able to grow on HA as the sole carbon source, seven on phenol and three, affiliated to the Roseobacter clade, on various aromatic acids. The bacteria retrieved from the flow-through cultures were closely (96-99%) affiliated to organisms capable of degrading humic matter, aromatic and aliphatic compounds and also to other bacteria reported previously from the Wadden Sea and Weser estuary.
Detection of Infections with Hepatitis B Virus, Hepatitis C Virus, and Human Immunodeficiency Virus by Analyses of Dried Blood Spots--performance Characteristics of the ARCHITECT System and Two Commercial Assays for Nucleic Acid Amplification Virology Journal. 2013 | Pubmed ID: 23497102 Nowadays, dried blood spots (DBS) are primarily used to obtain diagnostic access to risk collectives such as intravenous drug users, who are prone to infections with hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). Before DBS analyses can be used in this diagnostic context, however, a comprehensive evaluation of its performance characteristics must be conducted. To the best of our knowledge, the current study presents for the first time such essential data for the Abbott ARCHITECT system, which is currently the worldwide leading platform in this field of infection diagnostics.
Clinical Performance of the Novel DiaSorin LIAISON(®) XL Murex: HBsAg Quant, HCV-Ab, HIV-Ab/Ag Assays Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology. Jan, 2014 | Pubmed ID: 24268764 The fully automated and closed LIAISON(®)XL platform was developed for reliable detection of infection markers like hepatitis B virus (HBV) surface antigen (HBsAg), hepatitis C virus (HCV) antibodies (Ab) or human immunodeficiency virus (HIV)-Ag/Ab. To date, less is known about the diagnostic performance of this system in direct comparison to the common Abbott ARCHITECT(®) platform.