Other Publications (1)
Articles by Nicole M. McFarlane in JoVE
Behandling af primær hjernetumor Tissue for stamcelle Analyser og Flow Sortering Chitra Venugopal1, Nicole M. McFarlane1, Sara Nolte1, Branavan Manoranjan1, Sheila K. Singh1 1Stem Cell and Cancer Research Institute, McMaster University Identifikationen af hjernesvulst initierende celler (BTICs), de sjældne celler i en heterogene tumor besidder stamceller egenskaber, giver ny indsigt i den menneskelige hjerne tumor patogenese. Vi har forfinet særlige dyrkningsbetingelser at berige for BTICs, og vi rutinemæssigt bruger flowcytometri til yderligere berige disse befolkningsgrupper. Selvfornyelse assays og transkript analyse ved enkelt celle RT-PCR kan efterfølgende udføres på disse isolerede celler.
Other articles by Nicole M. McFarlane on PubMed
Radiation-induced Apoptosis in Mouse Lymphocytes is Modified by a Complex Dietary Supplement: the Effect of Genotype and Gender Mutagenesis. Nov, 2008 | Pubmed ID: 18644835 This study examined whether radiation sensitivity measured by lymphocyte apoptosis could be ameliorated by a complex anti-oxidant/anti-ageing dietary supplement. We also examined lymphocytes from both genders of normal (Nr) mice as well as transgenic growth hormone (Tg) mice that express strongly elevated reactive oxygen species processes and a progeroid syndrome of accelerated ageing. We introduce Tg mice as a potentially valuable new model to study radiation sensitivity. Isolated lymphocytes from all experimental groups were exposed to gamma radiation and the time course of apoptosis was measured in vitro. Kinetics of radiation-induced apoptosis was similar among groups, which peaked at 8 h, but maximal levels differed significantly between groups. Nr male mice had 60% lower levels of radiation-induced apoptosis than Tg males, supporting our hypothesis that Tg mice would be radiation sensitive. The dietary supplement protected lymphocytes in male mice of both strains, with proportionally greater reductions in Tg mice. Lymphocytes from female mice (both Nr and Tg) were highly radiation resistant compared to males and the supplement provided no additional benefit at the doses used in this study. These results highlight that radiation-induced apoptosis is complex and is modified by genotype, dietary supplements and gender.