Articles by Nikola Cesarovic in JoVE
Implantatie van radiotelemetrie zenders Opbrengst Gegevens over ECG, hartslag, lichaamstemperatuur en activiteit in Free-bewegende laboratoriummuizen Nikola Cesarovic1, Paulin Jirkof2, Andreas Rettich2, Margarete Arras1 1Division of Surgical Research, University Hospital Zurich, 2Institute of Laboratory Animal Science, University of Zurich Een chirurgische techniek voor implantatie van in de handel verkrijgbaar telemetrie zenders gebruikt worden voor continue meting van biopotential (een-lead ECG), hartslag, lichaamstemperatuur en bewegingsactiviteit in vrij bewegende muizen wordt getoond. Aanbevelingen en protocollen voor de post-operatieve zorg en pijnbestrijding, het verbeteren van het herstel, het welzijn en overleving worden ook gepresenteerd.
Other articles by Nikola Cesarovic on PubMed
Isoflurane and Sevoflurane Provide Equally Effective Anaesthesia in Laboratory Mice Laboratory Animals. Oct, 2010 | Pubmed ID: 20507878 Isoflurane is currently the most common volatile anaesthetic used in laboratory mice, whereas in human medicine the more modern sevoflurane is often used for inhalation anaesthesia. This study aimed to characterize and compare the clinical properties of both anaesthetics for inhalation anaesthesia in mice. In an approach mirroring routine laboratory conditions (spontaneous breathing, gas supply via nose mask, preventing hypothermia by a warming mat) a 50 min anaesthesia was performed. Anaesthetics were administered in oxygen as carrier gas at standardized dosages of 1.5 minimum alveolar concentrations, which was 2.8% for isoflurane and 4.9% for sevoflurane. Both induction and recovery from anaesthesia proceeded quickly, within 1-2 min. During anaesthesia, all reflex testing was negative and no serious impairment of vital functions was found; all animals survived. The most prominent side-effect during anaesthesia was respiratory depression with hypercapnia, acidosis and a marked decrease in respiration rate. Under anaesthesia, heart rate and core body temperature remained within the normal range, but were significantly increased for 12 h after anaesthesia. Locomotor activity, daily food and water consumption and body weight progression showed no abnormalities after anaesthesia. No significant difference was found between the two anaesthetics. In conclusion, isoflurane and sevoflurane provided an equally reliable anaesthesia in laboratory mice.
Burrowing Behavior As an Indicator of Post-laparotomy Pain in Mice Frontiers in Behavioral Neuroscience. 2010 | Pubmed ID: 21031028 Detection of persistent pain of a mild-to-moderate degree in laboratory mice is difficult because mice do not show unambiguous symptoms of pain or suffering using standard methods of short-term observational or clinical monitoring. This study investigated the potential use of burrowing performance - a spontaneous and highly motivated behavior - as a measure of post-operative pain in laboratory mice. The influence of minor surgery on burrowing was investigated in adult C57BL/6J mice of both genders in a modified rodent burrowing test (displacement of food pellets from a pellet-filled tube) within the animal's home cage. Almost all (98%) healthy mice burrowed (mean latency 1.3â€‰h, SEM 0.5â€‰h). After surgery without pain treatment, latency of burrowing was significantly prolonged (mean Î” latency 10â€‰h). Analgesic treatment using the anti-inflammatory drug carprofen (5â€‰mg/kg bodyweight) decreased latency of burrowing after surgery (mean Î” latency 5.5â€‰h) to the level found in mice that had been anesthetized (mean Î” latency 5.4â€‰h) or had received anesthesia and analgesia (mean Î” latency 4.6â€‰h). Analgesia during surgery was associated with a significantly earlier onset of burrowing compared to surgery without pain treatment. A distinct gradation in burrowing performance was found ranging from the undisturbed pre-operative status to the intermediate level following anesthesia/analgesia and surgery with analgesia, to the pronounced prolongation of latency to burrow after surgery without pain relief. In conclusion, post-surgical impairment of general condition, probably mainly attributable to pain, can be conveniently assessed in laboratory mice on the basis of the burrowing test.
Skingineering II: Transplantation of Large-scale Laboratory-grown Skin Analogues in a New Pig Model Pediatric Surgery International. Mar, 2011 | Pubmed ID: 21069348 Tissue engineering of skin with near-normal anatomy is an intriguing novel strategy to attack the still unsolved problem of how to ideally cover massive full-thickness skin defects. After successful production of large, pig cell-derived skin analogues, we now aim at developing an appropriate large animal model for transplantation studies.