Nina Harmening

Experimental Ophthalmology

University of Geneva

Nina Harmening

Dr. rer. nat. Nina Harmening, PhD in biology, joined Prof. Thumann’s research group in 2009. She has extensive experience in the isolation and culture of primary cells and cell lines, non-viral gene delivery methodologies including electroporation, the use of the Sleeping Beauty transposon system, and molecular and biochemical analyses. She has been trained in GMP-compliant production of GTMPs (Gene Therapy Medicinal Product) for use in clinical trials, qualified to work under GMP-grade conditions and is the responsible person for production. Currently, she is working on the improvement of the safety profile of SB100X transfected cells.


Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute

JoVE 61987