Articles by Nishanth Lakshman in JoVE
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury Nishanth Lakshman1,2, Wenjun Xu2, Cindi M. Morshead1,2,3 1Institute of Medical Science, University of Toronto, 2Department of Surgery, University of Toronto, 3Institute of Biomaterials and Biomedical Engineering, University of Toronto Here, we demonstrate the performance of a minimal spinal cord injury model in an adult mouse that spares the central canal niche housing endogenous neural stem cells (NSCs). We show how the neurosphere assay can be used to quantify activation and migration of definitive and primitive NSCs following injury.
Other articles by Nishanth Lakshman on PubMed
Building a Central Nervous System: The Neural Stem Cell Lineage Revealed Neurogenesis (Austin, Tex.). 2017 | Pubmed ID: 28516107 Neural stem cells (NSCs) are a multipotent, self-renewing source of undifferentiated cells in the periventricular region of the mammalian central nervous system (CNS). Since their original discovery 25 years ago, much has been learned about their development, persistence, localization, properties and potential. Herein we discuss the current state of knowledge pertaining to neural stem cells with a focus on the lineage relationship between two NSC populations along the neuraxis and their regionally distinct niches in the CNS.