Articles by Noel Cruz Pacheco in JoVE
Manipulating the Murine Lacrimal Gland Jennifer K. Finley1, D'Juan Farmer1, Elaine Emmerson1, Noel Cruz Pacheco1, Sarah M. Knox1 1Craniofacial and Mesenchymal Biology, Cell and Tissue Biology, University of California San Francisco The lacrimal gland (LG) is a branching organ that produces the aqueous components of tears necessary for maintaining vision and ocular health. Here we describe murine LG dissection and ex vivo culture techniques to decipher signaling pathways involved in LG development.
Other articles by Noel Cruz Pacheco on PubMed
Parasympathetic Innervation Regulates Tubulogenesis in the Developing Salivary Gland Developmental Cell. Aug, 2014 | Pubmed ID: 25158854 A fundamental question in development is how cells assemble to form a tubular network during organ formation. In glandular organs, tubulogenesis is a multistep process requiring coordinated proliferation, polarization and reorganization of epithelial cells to form a lumen, and lumen expansion. Although it is clear that epithelial cells possess an intrinsic ability to organize into polarized structures, the mechanisms coordinating morphogenetic processes during tubulogenesis are poorly understood. Here, we demonstrate that parasympathetic nerves regulate tubulogenesis in the developing salivary gland. We show that vasoactive intestinal peptide (VIP) secreted by the innervating ganglia promotes ductal growth, leads to the formation of a contiguous lumen, and facilitates lumen expansion through a cyclic AMP/protein kinase A (cAMP/PKA)-dependent pathway. Furthermore, we provide evidence that lumen expansion is independent of apoptosis and involves the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated Cl(-) channel. Thus, parasympathetic innervation coordinates multiple steps in tubulogenesis during organogenesis.