Pablo Sobrado Department of Biochemistry Virginia Polytechnic Institute and State University Biography Publications Institution JoVE Articles Pablo Sobrado has not added a biography. If you are Pablo Sobrado and would like to personalize this page please email our Author Liaison for assistance. Publications A Fluorescence Polarization Binding Assay to Identify Inhibitors of Flavin-dependent Monooxygenases Analytical Biochemistry. Mar, 2012 | Pubmed ID: 22410281 Crystal Structures and Small-angle X-ray Scattering Analysis of UDP-galactopyranose Mutase from the Pathogenic Fungus Aspergillus Fumigatus The Journal of Biological Chemistry. Jan, 2012 | Pubmed ID: 22294687 Fluorescence Polarization Binding Assay for Aspergillus Fumigatus Virulence Factor UDP-Galactopyranose Mutase Enzyme Research. 2011 | Pubmed ID: 21876791 Substrate Binding Modulates the Activity of Mycobacterium Smegmatis G, a Flavin-dependent Monooxygenase Involved in the Biosynthesis of Hydroxamate-containing Siderophores Biochemistry. Oct, 2011 | Pubmed ID: 21870809 Biosynthesis of Galactofuranose in Kinetoplastids: Novel Therapeutic Targets for Treating Leishmaniasis and Chagas' Disease Enzyme Research. 2011 | Pubmed ID: 21687654 Isolation and Characterization of Functional Leishmania Major Virulence Factor UDP-galactopyranose Mutase Biochemical and Biophysical Research Communications. Apr, 2011 | Pubmed ID: 21419104 Characterization of Recombinant UDP-galactopyranose Mutase from Aspergillus Fumigatus Archives of Biochemistry and Biophysics. Oct, 2010 | Pubmed ID: 20615386 Aspergillus Fumigatus SidA is a Highly Specific Ornithine Hydroxylase with Bound Flavin Cofactor Biochemistry. Aug, 2010 | Pubmed ID: 20614882 Recombinant Expression, Purification, and Characterization of ThmD, the Oxidoreductase Component of Tetrahydrofuran Monooxygenase Archives of Biochemistry and Biophysics. Apr, 2010 | Pubmed ID: 20159007 Developmental Expression of Xenopus Myosin 1d and Identification of a Myo1d Tail Homology That Overlaps TH1 Development, Growth & Differentiation. May, 2009 | Pubmed ID: 19382939 A Protein Structure Initiative Approach to Expression, Purification, and in Situ Delivery of Human Cytochrome B5 to Membrane Vesicles Protein Expression and Purification. Apr, 2008 | Pubmed ID: 18226920 Mechanistic and Structural Studies of H373Q Flavocytochrome B2: Effects of Mutating the Active Site Base Biochemistry. Jul, 2007 | Pubmed ID: 17563122 Identification of the Binding Region of the [2Fe-2S] Ferredoxin in Stearoyl-acyl Carrier Protein Desaturase: Insight into the Catalytic Complex and Mechanism of Action Biochemistry. Apr, 2006 | Pubmed ID: 16605252 Basic Region of Residues 228-231 of Protein Kinase CK1alpha is Involved in Its Interaction with Axin: Binding to Axin Does Not Affect the Kinase Activity Journal of Cellular Biochemistry. Feb, 2005 | Pubmed ID: 15565646 Solvent and Primary Deuterium Isotope Effects Show That Lactate CH and OH Bond Cleavages Are Concerted in Y254F Flavocytochrome B2, Consistent with a Hydride Transfer Mechanism Biochemistry. Dec, 2003 | Pubmed ID: 14690431 Identification of Tyr413 As an Active Site Residue in the Flavoprotein Tryptophan 2-monooxygenase and Analysis of Its Contribution to Catalysis Biochemistry. Dec, 2003 | Pubmed ID: 14636050 Analysis of the Role of the Active Site Residue Arg98 in the Flavoprotein Tryptophan 2-monooxygenase, a Member of the L-amino Oxidase Family Biochemistry. Dec, 2003 | Pubmed ID: 14636049 Analysis of the Roles of Amino Acid Residues in the Flavoprotein Tryptophan 2-monooxygenase Modified by 2-oxo-3-pentynoate: Characterization of His338, Cys339, and Cys511 Mutant Enzymes Archives of Biochemistry and Biophysics. Jun, 2002 | Pubmed ID: 12051679 Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry Elvira Romero1, Reeder Robinson1, Pablo Sobrado1 1Department of Biochemistry, Virginia Polytechnic Institute and State University JoVE 3803 Bioengineering
Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry Elvira Romero1, Reeder Robinson1, Pablo Sobrado1 1Department of Biochemistry, Virginia Polytechnic Institute and State University JoVE 3803 Bioengineering