Pamela A. Silver Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering Harvard University Biography Publications Institution JoVE Articles Pamela A. Silver has not added a biography. If you are Pamela A. Silver and would like to personalize this page please email our Author Liaison for assistance. Publications Integrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go Cell. Mar, 2014 | Pubmed ID: 24679533 Programmable Bacteria Detect and Record an Environmental Signal in the Mammalian Gut Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24639514 HITS-CLIP and Integrative Modeling Define the Rbfox Splicing-Regulatory Network Linked to Brain Development and Autism Cell Reports. Mar, 2014 | Pubmed ID: 24613350 Synthetic Biology in Mammalian Cells: Next Generation Research Tools and Therapeutics Nature Reviews. Molecular Cell Biology. Feb, 2014 | Pubmed ID: 24434884 Rapid Construction of Insulated Genetic Circuits Via Synthetic Sequence-guided Isothermal Assembly Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24078086 Building Synthetic Cellular Organization Molecular Biology of the Cell. Dec, 2013 | Pubmed ID: 24288075 An Objective Function Exploiting Suboptimal Solutions in Metabolic Networks BMC Systems Biology. 2013 | Pubmed ID: 24088221 Building Synthetic Memory Current Biology : CB. Sep, 2013 | Pubmed ID: 24028965 The Bacterial Carbon-fixing Organelle is Formed by Shell Envelopment of Preassembled Cargo PloS One. 2013 | Pubmed ID: 24023971 Targeting a Heterologous Protein to Multiple Plant Organelles Via Rationally Designed 5' MRNA Tags Journal of Biological Engineering. 2013 | Pubmed ID: 24011257 Two- and Three-input TALE-based AND Logic Computation in Embryonic Stem Cells Nucleic Acids Research. Nov, 2013 | Pubmed ID: 23982518 Isothermal Self-assembly of Complex DNA Structures Under Diverse and Biocompatible Conditions Nano Letters. Sep, 2013 | Pubmed ID: 23978049 Dynamics Simulations for Engineering Macromolecular Interactions Chaos (Woodbury, N.Y.). Jun, 2013 | Pubmed ID: 23822508 Tailored Fatty Acid Synthesis Via Dynamic Control of Fatty Acid Elongation Proceedings of the National Academy of Sciences of the United States of America. Jul, 2013 | Pubmed ID: 23798438 Protein Scaffold-activated Protein Trans-splicing in Mammalian Cells Journal of the American Chemical Society. May, 2013 | Pubmed ID: 23621664 PQBP1, a Factor Linked to Intellectual Disability, Affects Alternative Splicing Associated with Neurite Outgrowth Genes & Development. Mar, 2013 | Pubmed ID: 23512658 Expression of the Sub-pathways of the Chloroflexus Aurantiacus 3-hydroxypropionate Carbon Fixation Bicycle in E. Coli: Toward Horizontal Transfer of Autotrophic Growth Metabolic Engineering. Mar, 2013 | Pubmed ID: 23376595 Spatial and Temporal Organization of Chromosome Duplication and Segregation in the Cyanobacterium Synechococcus Elongatus PCC 7942 PloS One. 2012 | Pubmed ID: 23112856 Designing and Using RNA Scaffolds to Assemble Proteins in Vivo Nature Protocols. Oct, 2012 | Pubmed ID: 22955695 Designing Biological Compartmentalization Trends in Cell Biology. Dec, 2012 | Pubmed ID: 22841504 Synthetic Memory Circuits for Tracking Human Cell Fate Genes & Development. Jul, 2012 | Pubmed ID: 22751502 STAR RNA-binding Protein Quaking Suppresses Cancer Via Stabilization of Specific MiRNA Genes & Development. Jul, 2012 | Pubmed ID: 22751500 A BioBrick Compatible Strategy for Genetic Modification of Plants Journal of Biological Engineering. 2012 | Pubmed ID: 22716313 Improving Carbon Fixation Pathways Current Opinion in Chemical Biology. Aug, 2012 | Pubmed ID: 22647231 Natural Strategies for the Spatial Optimization of Metabolism in Synthetic Biology Nature Chemical Biology. Jun, 2012 | Pubmed ID: 22596204 Ingeniería Sintéticas Efectores TAL Con Sitios De Destino Ortogonal Nucleic Acids Research. May, 2012 | Pubmed ID: 22581776 DNA Ends Alter the Molecular Composition and Localization of Ku Multicomponent Complexes Molecular & Cellular Proteomics : MCP. Aug, 2012 | Pubmed ID: 22535209 A Sensitive Switch for Visualizing Natural Gene Silencing in Single Cells ACS Synthetic Biology. Mar, 2012 | Pubmed ID: 22530199 Induction of Biogenic Magnetization and Redox Control by a Component of the Target of Rapamycin Complex 1 Signaling Pathway PLoS Biology. 2012 | Pubmed ID: 22389629 A Tunable Zinc Finger-based Framework for Boolean Logic Computation in Mammalian Cells Nucleic Acids Research. Jun, 2012 | Pubmed ID: 22323524 Rerouting Carbon Flux to Enhance Photosynthetic Productivity Applied and Environmental Microbiology. Apr, 2012 | Pubmed ID: 22307292 Modularity of a Carbon-fixing Protein Organelle Proceedings of the National Academy of Sciences of the United States of America. Jan, 2012 | Pubmed ID: 22184212 Parts Plus Pipes: Synthetic Biology Approaches to Metabolic Engineering Metabolic Engineering. May, 2012 | Pubmed ID: 22037345 Cell-to-cell Variability of Alternative RNA Splicing Molecular Systems Biology. 2011 | Pubmed ID: 21734645 Organization of Intracellular Reactions with Rationally Designed RNA Assemblies Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21700839 Recording Cellular Experiences of DNA Damage Cell Cycle (Georgetown, Tex.). Aug, 2011 | Pubmed ID: 21670599 Synthetic Reversal of Epigenetic Silencing The Journal of Biological Chemistry. Aug, 2011 | Pubmed ID: 21669865 Visualization of Single MRNAs Reveals Temporal Association of Proteins with MicroRNA-regulated MRNA Nucleic Acids Research. Sep, 2011 | Pubmed ID: 21653551 A Synthetic System Links FeFe-hydrogenases to Essential E. Coli Sulfur Metabolism Journal of Biological Engineering. 2011 | Pubmed ID: 21615937 Towards a Synthetic Chloroplast PloS One. 2011 | Pubmed ID: 21533097 Whole Genome SiRNA Cell-based Screen Links Mitochondria to Akt Signaling Network Through Uncoupling of Electron Transport Chain Molecular Biology of the Cell. May, 2011 | Pubmed ID: 21460183 Informing Biological Design by Integration of Systems and Synthetic Biology Cell. Mar, 2011 | Pubmed ID: 21414477 Rewiring Hydrogenase-dependent Redox Circuits in Cyanobacteria Proceedings of the National Academy of Sciences of the United States of America. Mar, 2011 | Pubmed ID: 21368150 Synthetic Circuit Identifies Subpopulations with Sustained Memory of DNA Damage Genes & Development. Mar, 2011 | Pubmed ID: 21363961 Engineering Cyanobacteria to Generate High-value Products Trends in Biotechnology. Feb, 2011 | Pubmed ID: 21211860 Modular Electron Transfer Circuits for Synthetic Biology: Insulation of an Engineered Biohydrogen Pathway Bioengineered Bugs. Nov-Dec, 2010 | Pubmed ID: 21468209 Dynamics in the Mixed Microbial Concourse Genes & Development. Dec, 2010 | Pubmed ID: 21123647 Genome-wide RNAi Screen Discovers Functional Coupling of Alternative Splicing and Cell Cycle Control to Apoptosis Regulation Cell Cycle (Georgetown, Tex.). Nov, 2010 | Pubmed ID: 21088477 A Structurally Tunable DNA-based Extracellular Matrix Journal of the American Chemical Society. Oct, 2010 | Pubmed ID: 20925350 Emergent Cooperation in Microbial Metabolism Molecular Systems Biology. Sep, 2010 | Pubmed ID: 20823845 Una Red Alternativa De Empalme Enlaces De Control Del Ciclo Celular a La Apoptosis Cell. Aug, 2010 | Pubmed ID: 20705336 Knowing when to Change: Reprogramming (my) Life Nature Cell Biology. Aug, 2010 | Pubmed ID: 20680000 Engineering Cyanobacteria to Synthesize and Export Hydrophilic Products Applied and Environmental Microbiology. Jun, 2010 | Pubmed ID: 20363793 Spatially Ordered Dynamics of the Bacterial Carbon Fixation Machinery Science (New York, N.Y.). Mar, 2010 | Pubmed ID: 20203050 Insulation of a Synthetic Hydrogen Metabolism Circuit in Bacteria Journal of Biological Engineering. 2010 | Pubmed ID: 20184755 A Synthetic Circuit for Selectively Arresting Daughter Cells to Create Aging Populations Nucleic Acids Research. May, 2010 | Pubmed ID: 20150416 Making Cellular Memories Cell. Jan, 2010 | Pubmed ID: 20085698 Anti-glycophorin Single-chain Fv Fusion to Low-affinity Mutant Erythropoietin Improves Red Blood Cell-lineage Specificity Protein Engineering, Design & Selection : PEDS. Apr, 2010 | Pubmed ID: 20083493 Eukaryotic Systems Broaden the Scope of Synthetic Biology The Journal of Cell Biology. Nov, 2009 | Pubmed ID: 19948487 Metazoan Stress Granule Assembly is Mediated by P-eIF2alpha-dependent and -independent Mechanisms RNA (New York, N.Y.). Oct, 2009 | Pubmed ID: 19661161 Systems-level Engineering of Nonfermentative Metabolism in Yeast Genetics. Sep, 2009 | Pubmed ID: 19564482 Synthetic Biology: Exploring and Exploiting Genetic Modularity Through the Design of Novel Biological Networks Molecular BioSystems. Jul, 2009 | Pubmed ID: 19562109 A Conserved CCCH-type Zinc Finger Protein Regulates MRNA Nuclear Adenylation and Export The Journal of Cell Biology. Apr, 2009 | Pubmed ID: 19364924 Harnessing Nature's Toolbox: Regulatory Elements for Synthetic Biology Journal of the Royal Society, Interface / the Royal Society. Aug, 2009 | Pubmed ID: 19324675 Engineering a Synthetic Dual-organism System for Hydrogen Production Applied and Environmental Microbiology. Apr, 2009 | Pubmed ID: 19201964 Learning a Prior on Regulatory Potential from EQTL Data PLoS Genetics. Jan, 2009 | Pubmed ID: 19180192 MRNA Nuclear Export and Human Disease Disease Models & Mechanisms. Sep-Oct, 2008 | Pubmed ID: 19048072 Intron Length Increases Oscillatory Periods of Gene Expression in Animal Cells Genes & Development. Sep, 2008 | Pubmed ID: 18703678 Intron Delays and Transcriptional Timing During Development Developmental Cell. Mar, 2008 | Pubmed ID: 18331713 Global Histone Acetylation Induces Functional Genomic Reorganization at Mammalian Nuclear Pore Complexes Genes & Development. Mar, 2008 | Pubmed ID: 18316479 Enhancement of Cell Type Specificity by Quantitative Modulation of a Chimeric Ligand The Journal of Biological Chemistry. Mar, 2008 | Pubmed ID: 18230610 Defossiling Fuel: How Synthetic Biology Can Transform Biofuel Production ACS Chemical Biology. Jan, 2008 | Pubmed ID: 18205286 CARM1 Regulates Estrogen-stimulated Breast Cancer Growth Through Up-regulation of E2F1 Cancer Research. Jan, 2008 | Pubmed ID: 18172323 Definition of Global and Transcript-specific MRNA Export Pathways in Metazoans Genes & Development. Jan, 2008 | Pubmed ID: 18086857 Análisis Global De Empalme Del ARNm RNA (New York, N.Y.). Feb, 2008 | Pubmed ID: 18083834 Coupling and Coordination in Gene Expression Processes: a Systems Biology View Nature Reviews. Genetics. Jan, 2008 | Pubmed ID: 18071322 Especificidad Funcional Entre Proteínas Ribosomales Regula La Expresión Génica Cell. Nov, 2007 | Pubmed ID: 17981122 Rational Design of Memory in Eukaryotic Cells Genes & Development. Sep, 2007 | Pubmed ID: 17875664 Exon Expression Profiling Reveals Stimulus-mediated Exon Use in Neural Cells Genome Biology. 2007 | Pubmed ID: 17683528 Systems Perspectives on MRNA Processing Cell Research. Jul, 2007 | Pubmed ID: 17621309 Genetically Encoded Short Peptide Tags for Orthogonal Protein Labeling by Sfp and AcpS Phosphopantetheinyl Transferases ACS Chemical Biology. May, 2007 | Pubmed ID: 17465518 Why Do So Few Women Speak at Science Meetings? Nature. Apr, 2007 | Pubmed ID: 17443161 Transcriptional Regulation at the Nuclear Pore Complex Current Opinion in Genetics & Development. Apr, 2007 | Pubmed ID: 17317147 Designing Biological Systems Genes & Development. Feb, 2007 | Pubmed ID: 17289915 Solenopsin, the Alkaloidal Component of the Fire Ant (Solenopsis Invicta), is a Naturally Occurring Inhibitor of Phosphatidylinositol-3-kinase Signaling and Angiogenesis Blood. Jan, 2007 | Pubmed ID: 16990598 Contratación Diferencial De La Maquinaria De Empalme Durante La Transcripción Predice Todo El Genoma Patrones De Empalme Del ARNm Molecular Cell. Dec, 2006 | Pubmed ID: 17189192 The Role of Protein Arginine Methylation in the Formation of Silent Chromatin Genes & Development. Dec, 2006 | Pubmed ID: 17158743 Genome-wide Identification of Functionally Distinct Subsets of Cellular MRNAs Associated with Two Nucleocytoplasmic-shuttling Mammalian Splicing Factors Genome Biology. 2006 | Pubmed ID: 17137510 Communication Between Levels of Transcriptional Control Improves Robustness and Adaptivity Molecular Systems Biology. 2006 | Pubmed ID: 17130867 The Unfolded Protein Response Transducer Ire1p Contains a Nuclear Localization Sequence Recognized by Multiple Beta Importins Molecular Biology of the Cell. Dec, 2006 | Pubmed ID: 17035634 Genome-wide Analysis of Estrogen Receptor Binding Sites Nature Genetics. Nov, 2006 | Pubmed ID: 17013392 Transcriptional Regulation by the Proteasome As a Mechanism for Cellular Protein Homeostasis Cell Cycle (Georgetown, Tex.). Jul, 2006 | Pubmed ID: 16861887 The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-associated Proteins in Saccharomyces Cerevisiae Genetics. Sep, 2006 | Pubmed ID: 16816426 Genomic Localization of RNA Binding Proteins Reveals Links Between Pre-mRNA Processing and Transcription Genome Research. Jul, 2006 | Pubmed ID: 16769980 Systems Biology of Gene Regulation Fulfills Its Promise Genome Biology. 2006 | Pubmed ID: 16719937 Pore-ing the Right Dose Nature Cell Biology. May, 2006 | Pubmed ID: 16691206 Motion As a Phenotype: the Use of Live-cell Imaging and Machine Visual Screening to Characterize Transcription-dependent Chromosome Dynamics BMC Cell Biology. 2006 | Pubmed ID: 16635267 Genomic Association of the Proteasome Demonstrates Overlapping Gene Regulatory Activity with Transcription Factor Substrates Molecular Cell. Mar, 2006 | Pubmed ID: 16543154 Autoregulation of Ribosome Biosynthesis by a Translational Response in Fission Yeast Molecular and Cellular Biology. Mar, 2006 | Pubmed ID: 16478994 Biochemical and Genetic Analyses of Integrase-interacting Proteins Lens Epithelium-derived Growth Factor (LEDGF)/p75 and Hepatoma-derived Growth Factor Related Protein 2 (HRP2) in Preintegration Complex Function and HIV-1 Replication Virology. Mar, 2006 | Pubmed ID: 16337983 The Uses of Green Fluorescent Protein in Yeasts Methods of Biochemical Analysis. 2006 | Pubmed ID: 16335714 Multiple Conformations in the Ligand-binding Site of the Yeast Nuclear Pore-targeting Domain of Nup116p The Journal of Biological Chemistry. Oct, 2005 | Pubmed ID: 16105837 Genomic Mapping of RNA Polymerase II Reveals Sites of Co-transcriptional Regulation in Human Cells Genome Biology. 2005 | Pubmed ID: 16086846 A Genome-wide in Situ Hybridization Map of RNA-binding Proteins Reveals Anatomically Restricted Expression in the Developing Mouse Brain BMC Developmental Biology. 2005 | Pubmed ID: 16033648 Chromosome-wide Mapping of Estrogen Receptor Binding Reveals Long-range Regulation Requiring the Forkhead Protein FoxA1 Cell. Jul, 2005 | Pubmed ID: 16009131 Ufd1 Exhibits the AAA-ATPase Fold with Two Distinct Ubiquitin Interaction Sites Structure (London, England : 1993). Jul, 2005 | Pubmed ID: 16004872 Developmentally Induced Changes in Transcriptional Program Alter Spatial Organization Across Chromosomes Genes & Development. May, 2005 | Pubmed ID: 15905407 The Psammaplysenes, Specific Inhibitors of FOXO1a Nuclear Export Journal of Natural Products. Apr, 2005 | Pubmed ID: 15844952 HIV-1 Incorporates and Proteolytically Processes Human NDR1 and NDR2 Serine-threonine Kinases Virology. Jan, 2005 | Pubmed ID: 15582665 A Systems View of MRNP Biology Genes & Development. Dec, 2004 | Pubmed ID: 15574591 Class II Integrase Mutants with Changes in Putative Nuclear Localization Signals Are Primarily Blocked at a Postnuclear Entry Step of Human Immunodeficiency Virus Type 1 Replication Journal of Virology. Dec, 2004 | Pubmed ID: 15542626 Genome-wide MRNA Surveillance is Coupled to MRNA Export Genes & Development. Nov, 2004 | Pubmed ID: 15489286 Identification of an Evolutionarily Conserved Domain in Human Lens Epithelium-derived Growth Factor/transcriptional Co-activator P75 (LEDGF/p75) That Binds HIV-1 Integrase The Journal of Biological Chemistry. Nov, 2004 | Pubmed ID: 15371438 Human Cell Proteins and Human Immunodeficiency Virus DNA Integration Frontiers in Bioscience : a Journal and Virtual Library. Sep, 2004 | Pubmed ID: 15353349 Arginine Methyltransferase Affects Interactions and Recruitment of MRNA Processing and Export Factors Genes & Development. Aug, 2004 | Pubmed ID: 15314027 REF-ereeing the Cytoplasmic Fate of MRNA Via Nuclear Export Developmental Cell. Jun, 2004 | Pubmed ID: 15177019 PRMT3 is a Ribosomal Protein Methyltransferase That Affects the Cellular Levels of Ribosomal Subunits The EMBO Journal. Jul, 2004 | Pubmed ID: 15175657 Genome-wide Localization of the Nuclear Transport Machinery Couples Transcriptional Status and Nuclear Organization Cell. May, 2004 | Pubmed ID: 15137937 Guardian at the Gate: Preventing Unspliced Pre-mRNA Export Trends in Cell Biology. May, 2004 | Pubmed ID: 15130577 Human Mob Proteins Regulate the NDR1 and NDR2 Serine-threonine Kinases The Journal of Biological Chemistry. Jun, 2004 | Pubmed ID: 15067004 Therapeutic Potential of Retroviral RNAi Vectors Expert Opinion on Biological Therapy. Mar, 2004 | Pubmed ID: 15006726 Nuclear Transport and Cancer: from Mechanism to Intervention Nature Reviews. Cancer. Feb, 2004 | Pubmed ID: 14732865 Modulation of Interprotomer Relationships is Important for Activation of Dimeric Calcium-sensing Receptor The Journal of Biological Chemistry. Apr, 2004 | Pubmed ID: 14729680 A Chemical Genetic Screen Identifies Inhibitors of Regulated Nuclear Export of a Forkhead Transcription Factor in PTEN-deficient Tumor Cells Cancer Cell. Dec, 2003 | Pubmed ID: 14706338 Small Molecule Modulation of the Human Chromatid Decatenation Checkpoint Chemistry & Biology. Dec, 2003 | Pubmed ID: 14700634 A Subset of Membrane-associated Proteins is Ubiquitinated in Response to Mutations in the Endoplasmic Reticulum Degradation Machinery Proceedings of the National Academy of Sciences of the United States of America. Oct, 2003 | Pubmed ID: 14557538 Intracellular Transport of Human Immunodeficiency Virus Type 1 Integrase Journal of Cell Science. Nov, 2003 | Pubmed ID: 13130095 Analysis of RNA-protein Interactions by Flow Cytometry Current Opinion in Molecular Therapeutics. Jun, 2003 | Pubmed ID: 12870432 Sac3 is an MRNA Export Factor That Localizes to Cytoplasmic Fibrils of Nuclear Pore Complex Molecular Biology of the Cell. Mar, 2003 | Pubmed ID: 12631707 Nuclear Transport As a Target for Cell Growth Drug Discovery Today. Jan, 2003 | Pubmed ID: 12565010 Genome-wide Analysis of RNA-protein Interactions Illustrates Specificity of the MRNA Export Machinery Nature Genetics. Feb, 2003 | Pubmed ID: 12524544 Bipartite Signals Mediate Subcellular Targeting of Tail-anchored Membrane Proteins in Saccharomyces Cerevisiae The Journal of Biological Chemistry. Mar, 2003 | Pubmed ID: 12514182 A Microbead-based System for Identifying and Characterizing RNA-protein Interactions by Flow Cytometry Molecular & Cellular Proteomics : MCP. Dec, 2002 | Pubmed ID: 12543929 In Situ Analysis of Spatial Relationships Between Proteins of the Nuclear Pore Complex Biophysical Journal. Dec, 2002 | Pubmed ID: 12496130 Intron Status and 3'-end Formation Control Cotranscriptional Export of MRNA Genes & Development. Nov, 2002 | Pubmed ID: 12414728 Nuclear Localization of Human Immunodeficiency Virus Type 1 Preintegration Complexes (PICs): V165A and R166A Are Pleiotropic Integrase Mutants Primarily Defective for Integration, Not PIC Nuclear Import Journal of Virology. Nov, 2002 | Pubmed ID: 12368302 Retrovirus-delivered SiRNA BMC Biotechnology. Aug, 2002 | Pubmed ID: 12199908 Requirements for the Nuclear Export of the Small Ribosomal Subunit Journal of Cell Science. Jul, 2002 | Pubmed ID: 12082158 Nuclear Protein Transport Methods in Enzymology. 2002 | Pubmed ID: 12073370 Identifying Proteins That Affect MRNA Localization in Living Cells Methods (San Diego, Calif.). Feb, 2002 | Pubmed ID: 12054891 Identification of a Role for Saccharomyces Cerevisiae Cgr1p in Pre-rRNA Processing and 60S Ribosome Subunit Synthesis Microbiology (Reading, England). Apr, 2002 | Pubmed ID: 11932453 La Localización Del Genoma De Rsc9, Un Componente Del Complejo De Remodelación De La Cromatina RSC, Cambios En La Respuesta Al Estrés Molecular Cell. Mar, 2002 | Pubmed ID: 11931764 Protein and RNA Export from the Nucleus Developmental Cell. Mar, 2002 | Pubmed ID: 11879632 Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-binding Protein and Protein Kinase C Signaling Genetics. Feb, 2002 | Pubmed ID: 11861547 Expresión génica transitoria en tabaco utilizando Asamblea Gibson y la pistola de genes Matthew D. Mattozzi1,2, Mathias J. Voges1,2,3, Pamela A. Silver1,2, Jeffrey C. Way1,2 1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Systems Biology, Harvard Medical School, 3Department of Biotechnology, Delft University of Technology JoVE 51234 Environment
Expresión génica transitoria en tabaco utilizando Asamblea Gibson y la pistola de genes Matthew D. Mattozzi1,2, Mathias J. Voges1,2,3, Pamela A. Silver1,2, Jeffrey C. Way1,2 1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Systems Biology, Harvard Medical School, 3Department of Biotechnology, Delft University of Technology JoVE 51234 Environment
