In JoVE (1)
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Articles by Patricia M. Niemietz in JoVE
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice Felix Heymann*1, Patricia M. Niemietz*1, Julia Peusquens1, Can Ergen1, Marlene Kohlhepp1, Jana C. Mossanen1, Carlo Schneider1, Michael Vogt2, Rene H. Tolba3, Christian Trautwein1, Christian Martin4, Frank Tacke1 1Department of Medicine III, RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging", RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, 4Institute for Pharmacology, RWTH University-Hospital Aachen Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.