In JoVE (1)
Articles by Perumal Srinivasan Sureshkumar in JoVE
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation Vaibhav Shinde*1, Stefanie Klima*2, Perumal Srinivasan Sureshkumar1, Kesavan Meganathan1, Smita Jagtap1, Eugen Rempel3, Jörg Rahnenführer3, Jan Georg Hengstler4, Tanja Waldmann2, Jürgen Hescheler1, Marcel Leist*2, Agapios Sachinidis*1 1Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, 2Department of Biology, University of Konstanz, 3Department of Statistics, Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund The protocols describe two in vitro developmental toxicity test systems (UKK and UKN1) based on human embryonic stem cells and transcriptome studies. The test systems predict human developmental toxicity hazard, and may contribute to reduce animal studies, costs and the time required for chemical safety testing.
Other articles by Perumal Srinivasan Sureshkumar on PubMed
Enhanced Gap Junction Expression in Myoblast-containing Engineered Tissue Biochemical and Biophysical Research Communications. Jun, 2012 | Pubmed ID: 22579687 Transplantation of skeletal myoblasts (SMs) has been investigated as a potential cardiac cell therapy approach. SM are available autologously, predetermined for muscular differentiation and resistant to ischemia. Major hurdles for their clinical application are limitations in purity and yield during cell isolation as well as the absence of gap junction expression after differentiation into myotubes. Furthermore, transplanted SMs do not functionally or electrically integrate with the host myocardium. Here, we describe an efficient method for isolating homogeneous SM populations from neonatal mice and demonstrate persistent gap junction expression in an engineered tissue. This method resulted in a yield of 1.4 × 10(8) high-purity SMs (>99% desmin positive) after 10 days in culture from 162.12 ± 11.85 mg muscle tissue. Serum starvation conditions efficiently induced differentiation into spontaneously contracting myotubes that coincided with loss of gap junction expression. For mechanical conditioning, cells were integrated into engineered tissue constructs. SMs within tissue constructs exhibited long term survival, ordered alignment, and a preserved ability to differentiate into contractile myotubes. When the tissue constructs were subjected to passive longitudinal tensile stress, the expression of gap junction and cell adherence proteins was maintained or increased throughout differentiation. Our studies demonstrate that mechanical loading of SMs may provide for improved electromechanical integration within the myocardium, which could lead to more therapeutic opportunities.
Mechanical Preconditioning Enables Electrophysiologic Coupling of Skeletal Myoblast Cells to Myocardium The Journal of Thoracic and Cardiovascular Surgery. Nov, 2012 | Pubmed ID: 22980065 The effect of mechanical preconditioning on skeletal myoblasts in engineered tissue constructs was investigated to resolve issues associated with conduction block between skeletal myoblast cells and cardiomyocytes.
Preconditioning of Skeletal Myoblast-based Engineered Tissue Constructs Enables Functional Coupling to Myocardium In vivo The Journal of Thoracic and Cardiovascular Surgery. Jan, 2015 | Pubmed ID: 25439779 Skeletal myoblasts fuse to form functional syncytial myotubes as an integral part of the skeletal muscle. During this differentiation process, expression of proteins for mechanical and electrical integration is seized, which is a major drawback for the application of skeletal myoblasts in cardiac regenerative cell therapy, because global heart function depends on intercellular communication.