Articles by Pietro Ariani in JoVE
Flujo de trabajo integral para la identificación de todo el genoma y Meta-análisis de la expresión de la familia del Gene del ATL E3 ubiquitina ligasa en Grapevine Pietro Ariani*1, Elodie Vandelle*1, Darren Wong2, Alejandro Giorgetti1, Andrea Porceddu3, Salvatore Camiolo3, Annalisa Polverari1 1Dipartimento di Biotecnologie, Università degli Studi di Verona, 2Ecology and Evolution, Research School of Biology, The Australian National University, 3Dipartimento di Agraria, SACEG, Università degli Studi di Sassari Este artículo describe el procedimiento para la identificación y caracterización de una familia del gene en grapevine aplicada a la familia de Arabidopsis Tóxicos en Levadura (ATL) E3 ubiquitina ligasas.
Other articles by Pietro Ariani on PubMed
Genome-wide Characterisation and Expression Profile of the Grapevine ATL Ubiquitin Ligase Family Reveal Biotic and Abiotic Stress-responsive and Development-related Members Scientific Reports. | Pubmed ID: 27910910 The Arabidopsis Tóxicos en Levadura (ATL) protein family is a class of E3 ubiquitin ligases with a characteristic RING-H2 Zn-finger structure that mediates diverse physiological processes and stress responses in plants. We carried out a genome-wide survey of grapevine (Vitis vinifera L.) ATL genes and retrieved 96 sequences containing the canonical ATL RING-H2 domain. We analysed their genomic organisation, gene structure and evolution, protein domains and phylogenetic relationships. Clustering revealed several clades, as already reported in Arabidopsis thaliana and rice (Oryza sativa), with an expanded subgroup of grapevine-specific genes. Most of the grapevine ATL genes lacked introns and were scattered among the 19 chromosomes, with a high level of duplication retention. Expression profiling revealed that some ATL genes are expressed specifically during early or late development and may participate in the juvenile to mature plant transition, whereas others may play a role in pathogen and/or abiotic stress responses, making them key candidates for further functional analysis. Our data offer the first genome-wide overview and annotation of the grapevine ATL family, and provide a basis for investigating the roles of specific family members in grapevine physiology and stress responses, as well as potential biotechnological applications.