In JoVE (1)
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Articles by Pooja Sharma in JoVE
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies Pooja Sharma1,2, Mariam Kaywan-Lutfi1, Logesvaran Krshnan1,2, Eamon F. X. Byrne1,2, Melissa Joy Call1,2, Matthew Edwin Call1,2 1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.