Articles by Pooja Sharma in JoVE

• Chemistry

  
  Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies Pooja Sharma^1,2, Mariam Kaywan-Lutfi¹, Logesvaran Krshnan^1,2, Eamon F. X. Byrne^1,2, Melissa Joy Call^1,2, Matthew Edwin Call^{1,2 1}Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, ²The University of Melbourne Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.