Articles by Qingfa Peng in JoVE
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers Qingfa Peng1, Huili Shao1, Xuechao Hu1, Yaopeng Zhang1 1State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.
Other articles by Qingfa Peng on PubMed
Tough Silk Fibers Prepared in Air Using a Biomimetic Microfluidic Chip International Journal of Biological Macromolecules. May, 2014 | Pubmed ID: 24613677 Microfluidic chips with single channel were built to mimic the shear and elongation conditions in the spinning apparatus of spider and silkworm. Silk fibers dry-spun from regenerated silk fibroin (RSF) aqueous solution using the chip could be tougher than degummed natural silk. The artificial silk exhibited a breaking strength up to 614 MPa, a breaking elongation up to 27% and a breaking energy of 101 kJ/kg.
Recombinant Spider Silk from Aqueous Solutions Via a Bio-inspired Microfluidic Chip Scientific Reports. Nov, 2016 | Pubmed ID: 27819339 Spiders achieve superior silk fibres by controlling the molecular assembly of silk proteins and the hierarchical structure of fibres. However, current wet-spinning process for recombinant spidroins oversimplifies the natural spinning process. Here, water-soluble recombinant spider dragline silk protein (with a low molecular weight of 47 kDa) was adopted to prepare aqueous spinning dope. Artificial spider silks were spun via microfluidic wet-spinning, using a continuous post-spin drawing process (WS-PSD). By mimicking the natural spinning apparatus, shearing and elongational sections were integrated in the microfluidic spinning chip to induce assembly, orientation of spidroins, and fibril structure formation. The additional post-spin drawing process following the wet-spinning section partially mimics the spinning process of natural spider silk and substantially contributes to the compact aggregation of microfibrils. Subsequent post-stretching further improves the hierarchical structure of the fibres, including the crystalline structure, orientation, and fibril melting. The tensile strength and elongation of post-treated fibres reached up to 510 MPa and 15%, respectively.