Other Publications (1)
Articles by Rachel J. Jerrell in JoVE
कैंसर की कोशिकाओं पर Invadopodia और कर्षण बल Assays के लिए polyacrylamide जैल Rachel J. Jerrell1, Aron Parekh1 1Department of Otolaryngology, Vanderbilt University Medical Center ट्यूमर microenvironment में यांत्रिक कठोरता invadopodia गतिविधियों में वृद्धि और सिकुड़ना actomyosin द्वारा घातक व्यवहार ड्राइविंग में एक महत्वपूर्ण भूमिका निभाता है। का प्रयोग polyacrylamide जैल (पास), invadopodia और कर्षण बल assays के कठोरता मैट्रिक्स के जवाब में कैंसर कोशिकाओं के आक्रामक और सिकुड़ा गुणों का अध्ययन करने के लिए उपयोग किया जा सकता है।
Other articles by Rachel J. Jerrell on PubMed
Cellular Traction Stresses Mediate Extracellular Matrix Degradation by Invadopodia Acta Biomaterialia. May, 2014 | Pubmed ID: 24412623 During tumorigenesis, matrix rigidity can drive oncogenic transformation via altered cellular proliferation and migration. Cells sense extracellular matrix (ECM) mechanical properties with intracellular tensile forces generated by actomyosin contractility. These contractile forces are transmitted to the matrix surface as traction stresses, which mediate mechanical interactions with the ECM. Matrix rigidity has been shown to increase proteolytic ECM degradation by cytoskeletal structures known as invadopodia that are critical for cancer progression, suggesting that cellular contractility promotes invasive behavior. However, both increases and decreases in traction stresses have been associated with metastatic behavior. Therefore, the role of cellular contractility in invasive migration leading to metastasis is unclear. To determine the relationship between cellular traction stresses and invadopodia activity, we characterized the invasive and contractile properties of an aggressive carcinoma cell line utilizing polyacrylamide gels of different rigidities. We found that ECM degradation and traction stresses were linear functions of matrix rigidity. Using calyculin A to augment myosin contractility, we also found that traction stresses were strongly predictive of ECM degradation. Overall, our data suggest that cellular force generation may play an important part in invasion and metastasis by mediating invadopodia activity in response to the mechanical properties of the tumor microenvironment.