Other Publications (1)
Articles by Radha Muddu in JoVE
Rapid PCR Thermocycling using Microscale Thermal Convection Radha Muddu1, Yassin A. Hassan2, Victor M. Ugaz3 1Department of Mechanical Engineering, Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering, Texas A&M University, 3Department of Chemical Engineering, Texas A&M University We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.