Other Publications (1)
Articles by Raphaela Lohaus in JoVE
Coculture System med en Organotypic Brain Slice og 3D Spheroid av karcinomceller Han-Ning Chuang1, Raphaela Lohaus1, Uwe-Karsten Hanisch2, Claudia Binder1, Faramarz Dehghani*3, Tobias Pukrop*1 1Department of Hematology and Oncology, University of Göttingen, 2Institute of Neuropathology, University of Göttingen, 3Institute of Anatomy and Cellbiology, University of Halle Den organotypic hjernen skive coculture med karcinomceller muliggjør visualisere morfologiske endringer ved fluorescens samt lyse felt (video) mikros under prosessen med carcinoma celle invasjon av hjernevev. Denne modellen systemet gir også mulighet for celle utveksling og påfyll tilnærminger, og tilbyr et bredt utvalg av manipulasjoner og analyser.
Other articles by Raphaela Lohaus on PubMed
Microglia Promote Colonization of Brain Tissue by Breast Cancer Cells in a Wnt-dependent Way Glia. Sep, 2010 | Pubmed ID: 20549749 Although there is increasing evidence that blood-derived macrophages support tumor progression, it is still unclear whether specialized resident macrophages, such as brain microglia, also play a prominent role in metastasis formation. Here, we show that microglia enhance invasion and colonization of brain tissue by breast cancer cells, serving both as active transporters and guiding rails. This is antagonized by inactivation of microglia as well as by the Wnt inhibitor Dickkopf-2. Proinvasive microglia demonstrate altered morphology, but neither upregulation of M2-like cytokines nor differential gene expression. Bacterial lipopolysacharide shifts tumor-educated microglia into a classical M1 phenotype, reduces their proinvasive function, and unmasks inflammatory and Wnt signaling as the most strongly regulated pathways. Histological findings in human brain metastases underline the significance of these results. In conclusion, microglia are critical for the successful colonization of the brain by epithelial cancer cells, suggesting inhibition of proinvasive microglia as a promising antimetastatic strategy.