Raymond J. Owens

Publications

• GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System
  Methods in Molecular Biology (Clifton, N.J.). 2015  |   Pubmed ID: 25502201
• Transient Expression in HEK 293 Cells: An Alternative to E. Coli for the Production of Secreted and Intracellular Mammalian Proteins
  Methods in Molecular Biology (Clifton, N.J.). 2015  |   Pubmed ID: 25447866
• Adhiron: a Stable and Versatile Peptide Display Scaffold for Molecular Recognition Applications
  Protein Engineering, Design & Selection : PEDS. May, 2014  |   Pubmed ID: 24668773
• Application of In-Fusion™ Cloning for the Parallel Construction of E. Coli Expression Vectors
  Methods in Molecular Biology (Clifton, N.J.). 2014  |   Pubmed ID: 24395367
• Chromophore-linked Substrate (CLS405): Probing Metallo-β-lactamase Activity and Inhibition
  ChemMedChem. Dec, 2013  |   Pubmed ID: 24166830
• A Streamlined Implementation of the Glutamine Synthetase-based Protein Expression System
  BMC Biotechnology. 2013  |   Pubmed ID: 24063773
• Binding of (5S)-penicilloic Acid to Penicillin Binding Protein 3
  ACS Chemical Biology. Oct, 2013  |   Pubmed ID: 23899657
• Assay Platform for Clinically Relevant Metallo-β-lactamases
  Journal of Medicinal Chemistry. Sep, 2013  |   Pubmed ID: 23898798
• Crystal Structure of Signal Regulatory Protein Gamma (SIRPγ) in Complex with an Antibody Fab Fragment
  BMC Structural Biology. 2013  |   Pubmed ID: 23826770
• S-Adenosyl-S-carboxymethyl-L-homocysteine: a Novel Cofactor Found in the Putative TRNA-modifying Enzyme CmoA
  Acta Crystallographica. Section D, Biological Crystallography. Jun, 2013  |   Pubmed ID: 23695253
• Expression of Recombinant Glycoproteins in Mammalian Cells: Towards an Integrative Approach to Structural Biology
  Current Opinion in Structural Biology. Jun, 2013  |   Pubmed ID: 23623336
• Structure of Ribose 5-phosphate Isomerase from the Probiotic Bacterium Lactobacillus Salivarius UCC118
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Dec, 2012  |   Pubmed ID: 23192019
• Structural and Functional Characterization of the Kindlin-1 Pleckstrin Homology Domain
  The Journal of Biological Chemistry. Dec, 2012  |   Pubmed ID: 23132860
• Outrunning Free Radicals in Room-temperature Macromolecular Crystallography
  Acta Crystallographica. Section D, Biological Crystallography. Jul, 2012  |   Pubmed ID: 22751666
• Structure of the Regulatory Domain of the LysR Family Regulator NMB2055 (MetR-like Protein) from Neisseria Meningitidis
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jul, 2012  |   Pubmed ID: 22750853
• In Situ Macromolecular Crystallography Using Microbeams
  Acta Crystallographica. Section D, Biological Crystallography. May, 2012  |   Pubmed ID: 22525757
• Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells
  Methods in Molecular Biology (Clifton, N.J.). 2012  |   Pubmed ID: 21987252
• Streamlining Homogeneous Glycoprotein Production for Biophysical and Structural Applications by Targeted Cell Line Development
  PloS One. 2011  |   Pubmed ID: 22174749
• Structure of New Delhi Metallo-β-lactamase 1 (NDM-1)
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Oct, 2011  |   Pubmed ID: 22102018
• XtalPiMS: a PiMS-based Web Application for the Management and Monitoring of Crystallization Trials
  Journal of Structural Biology. Aug, 2011  |   Pubmed ID: 21605683
• Expression of Protein Complexes Using Multiple Escherichia Coli Protein Co-expression Systems: a Benchmarking Study
  Journal of Structural Biology. Aug, 2011  |   Pubmed ID: 21382497
• Crystal Structures of Penicillin-binding Protein 3 from Pseudomonas Aeruginosa: Comparison of Native and Antibiotic-bound Forms
  Journal of Molecular Biology. Jan, 2011  |   Pubmed ID: 20974151
• Novel Structural Features in Two ZHX Homeodomains Derived from a Systematic Study of Single and Multiple Domains
  BMC Structural Biology. 2010  |   Pubmed ID: 20509910
• Structural and Functional Insights of RANKL-RANK Interaction and Signaling
  Journal of Immunology (Baltimore, Md. : 1950). Jun, 2010  |   Pubmed ID: 20483727
• The Structure of a Reduced Form of OxyR from Neisseria Meningitidis
  BMC Structural Biology. 2010  |   Pubmed ID: 20478059
• Improved Expression of Secreted and Membrane-targeted Proteins in Insect Cells
  Biotechnology and Applied Biochemistry. Jul, 2010  |   Pubmed ID: 20441568
• The Crystal Structure of NGO0477 from Neisseria Gonorrhoeae Reveals a Novel Protein Fold Incorporating a Helix-turn-helix Motif
  Proteins. May, 2010  |   Pubmed ID: 20196080
• Recent Advances in the Production of Proteins in Insect and Mammalian Cells for Structural Biology
  Journal of Structural Biology. Oct, 2010  |   Pubmed ID: 20153433
• Structure and Functionality in Flavivirus NS-proteins: Perspectives for Drug Design
  Antiviral Research. Aug, 2010  |   Pubmed ID: 19945487
• Genetic Modification of a Baculovirus Vector for Increased Expression in Insect Cells
  Cell Biology and Toxicology. Feb, 2010  |   Pubmed ID: 19655260
• Structural Plasticity of Eph Receptor A4 Facilitates Cross-class Ephrin Signaling
  Structure (London, England : 1993). Oct, 2009  |   Pubmed ID: 19836338
• Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication
  Journal of Virology. Dec, 2009  |   Pubmed ID: 19793813
• Crystallization and Preliminary X-ray Analysis of Mouse RANK and Its Complex with RANKL
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jun, 2009  |   Pubmed ID: 19478440
• The Structure of CrgA from Neisseria Meningitidis Reveals a New Octameric Assembly State for LysR Transcriptional Regulators
  Nucleic Acids Research. Aug, 2009  |   Pubmed ID: 19474343
• The Structure of NMB1585, a MarR-family Regulator from Neisseria Meningitidis
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Mar, 2009  |   Pubmed ID: 19255465
• The Production of Glycoproteins by Transient Expression in Mammalian Cells
  Methods in Molecular Biology (Clifton, N.J.). 2009  |   Pubmed ID: 18988030
• The Precise Engineering of Expression Vectors Using High-throughput In-Fusion PCR Cloning
  Methods in Molecular Biology (Clifton, N.J.). 2009  |   Pubmed ID: 18988019
• Rhabdovirus Matrix Protein Structures Reveal a Novel Mode of Self-association
  PLoS Pathogens. Dec, 2008  |   Pubmed ID: 19112510
• Generation of Baculovirus Vectors for the High-throughput Production of Proteins in Insect Cells
  Biotechnology and Bioengineering. Dec, 2008  |   Pubmed ID: 18781697
• Crystallization and Preliminary X-ray Analysis of CrgA, a LysR-type Transcriptional Regulator from Pathogenic Neisseria Meningitidis MC58
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Sep, 2008  |   Pubmed ID: 18765907
• Vaccinia Virus Proteins A52 and B14 Share a Bcl-2-like Fold but Have Evolved to Inhibit NF-kappaB Rather Than Apoptosis
  PLoS Pathogens. 2008  |   Pubmed ID: 18704168
• A Pipeline for the Production of Antibody Fragments for Structural Studies Using Transient Expression in HEK 293T Cells
  Protein Expression and Purification. Nov, 2008  |   Pubmed ID: 18662785
• The Crystal Structure of UMP Kinase from Bacillus Anthracis (BA1797) Reveals an Allosteric Nucleotide-binding Site
  Journal of Molecular Biology. Sep, 2008  |   Pubmed ID: 18625239
• Structures of an Alanine Racemase from Bacillus Anthracis (BA0252) in the Presence and Absence of (R)-1-aminoethylphosphonic Acid (L-Ala-P)
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. May, 2008  |   Pubmed ID: 18453697
• Expression, Purification and Crystallization of a Lyssavirus Matrix (M) Protein
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Apr, 2008  |   Pubmed ID: 18391421
• Structure of the Cold-shock Domain Protein from Neisseria Meningitidis Reveals a Strand-exchanged Dimer
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Apr, 2008  |   Pubmed ID: 18391418
• Protein Production and Purification
  Nature Methods. Feb, 2008  |   Pubmed ID: 18235434
• Semi-automated Microseeding of Nanolitre Crystallization Experiments
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jan, 2008  |   Pubmed ID: 18097093
• Structure of the Murray Valley Encephalitis Virus RNA Helicase at 1.9 Angstrom Resolution
  Protein Science : a Publication of the Protein Society. Oct, 2007  |   Pubmed ID: 17893366
• Crystal Structure of the Murray Valley Encephalitis Virus NS5 Methyltransferase Domain in Complex with Cap Analogues
  The Journal of General Virology. Aug, 2007  |   Pubmed ID: 17622627
• The Structure and Transcriptional Analysis of a Global Regulator from Neisseria Meningitidis
  The Journal of Biological Chemistry. May, 2007  |   Pubmed ID: 17374605
• Glycoprotein Structural Genomics: Solving the Glycosylation Problem
  Structure (London, England : 1993). Mar, 2007  |   Pubmed ID: 17355862
• A Versatile Ligation-independent Cloning Method Suitable for High-throughput Expression Screening Applications
  Nucleic Acids Research. 2007  |   Pubmed ID: 17317681
• Analysis of Variable N-glycosylation Site Occupancy in Glycoproteins by Liquid Chromatography Electrospray Ionization Mass Spectrometry
  Analytical Biochemistry. Feb, 2007  |   Pubmed ID: 17178093
• Lysine Methylation As a Routine Rescue Strategy for Protein Crystallization
  Structure (London, England : 1993). Nov, 2006  |   Pubmed ID: 17098187
• Implementation of Semi-automated Cloning and Prokaryotic Expression Screening: the Impact of SPINE
  Acta Crystallographica. Section D, Biological Crystallography. Oct, 2006  |   Pubmed ID: 17001088
• Structure of the PII Signal Transduction Protein of Neisseria Meningitidis at 1.85 A Resolution
  Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jun, 2006  |   Pubmed ID: 16754965
• Crystal Structure of Nitrogen Regulatory Protein IIANtr from Neisseria Meningitidis
  BMC Structural Biology. 2005  |   Pubmed ID: 16092953