In JoVE (1)
Articles by Rebecca Marin in JoVE
Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS Paolo Benigni1, Rebecca Marin1, Kathia Sandoval1, Piero Gardinali1, Francisco Fernandez-Lima1 1Chemistry and Biochemistry, Florida International University The low-energy water-accommodated fraction (LEWAF) of crude oil is a challenging system to analyze, because over time, this complex mixture undergoes chemical transformations. This protocol illustrates methods for the preparation of the LEWAF sample and for performing photo-irradiation and chemical analysis by trapped ion mobility spectrometry–FT-ICR MS.
Other articles by Rebecca Marin on PubMed
Towards Unsupervised Polyaromatic Hydrocarbons Structural Assignment from SA-TIMS-FTMS Data International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry. Oct, 2015 | Pubmed ID: 26525904 With the advent of high resolution ion mobility analyzers and their coupling to ultrahigh resolution mass spectrometers, there is a need to further develop a theoretical workflow capable of correlating experimental accurate mass and mobility measurements with tridimensional candidate structures. In the present work, a general workflow is described for unsupervised tridimensional structural assignment based on accurate mass measurements, mobility measurements, in silico 2D-3D structure generation, and theoretical mobility calculations. In particular, the potential of this workflow will be shown for the analysis of polyaromatic hydrocarbons from Coal Tar SRM 1597a using selected accumulation - trapped ion mobility spectrometry (SA-TIMS) coupled to Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). The proposed workflow can be adapted to different IMS scenarios, can utilize different collisional cross-section calculators and has the potential to include MS(n) and IMS(n) measurements for faster and more accurate tridimensional structural assignment.
Towards the Analysis of High Molecular Weight Proteins and Protein Complexes Using TIMS-MS International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry. Sep, 2016 | Pubmed ID: 27818614 In the present work, we demonstrate the potential and versatility of TIMS for the analysis of proteins, DNA-protein complexes and protein-protein complexes in their native and denatured states. In addition, we show that accurate CCS measurement are possible and in good agreement with previously reported CCS values using other IMS analyzers (