Other Publications (1)
Articles by Renaud Poincloux in JoVE
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions Anaïs Bouissou*1, Amsha Proag*1, Marion Portes1, Vanessa Soldan2, Stéphanie Balor2, Christophe Thibault3,4, Christophe Vieu3,4, Isabelle Maridonneau-Parini1, Renaud Poincloux1 1Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA Here, we detail the experimental techniques used to evaluate the protrusion forces that podosomes apply on a compliant film, from the preparation of the film to the automated analysis of topographical images.
Other articles by Renaud Poincloux on PubMed
Evaluation of the Force and Spatial Dynamics of Macrophage Podosomes by Multi-particle Tracking Methods (San Diego, Calif.). | Pubmed ID: 26342257 Podosomes are submicron adhesive and mechanosensitive structures formed by macrophages, dendritic cells and osteoclasts that are capable of protruding into the extracellular environment. Built of an F-actin core surrounded by an adhesion ring, podosomes assemble in a network interconnected by acto-myosin cables. They have been shown to display spatiotemporal instability as well as protrusion force oscillations. To analyse the entire population of these unstable structures, we have designed an automated multi-particle tracking adapted to both topographical and fluorescence data. Here we describe in detail this approach and report the measurements of individual and collective characteristics of podosome ensembles, providing an integrated picture of their activity from the complementary angles of organisation, dynamics, mobility and mechanics. We believe that this will lead to a comprehensive view of podosome collective behaviour and deepen our knowledge about the significance of mechanosensing mediated by protrusive structures.