In JoVE (1)
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Articles by Ricardo Hidalgo in JoVE
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase Philip A. Ash*1, Ricardo Hidalgo*1, Kylie A. Vincent1 1Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory Here, we describe a technique, protein film infrared electrochemistry, which allows immobilized redox proteins to be studied spectroscopically under direct electrochemical control at a carbon electrode. Infrared spectra of a single protein sample can be recorded at a range of applied potentials and under a variety of solution conditions.