Articles by Richard Bodine in JoVE
Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery Limor Man1, Laura Park1, Richard Bodine1, Michael Ginsberg2, Nikica Zaninovic3, Glenn Schattman1, Robert E. Schwartz4, Zev Rosenwaks1,3, Daylon James1,3 1Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, 2Angiocrine Biosciences, Inc., 3Tri-Institutional Stem Cell Derivation Laboratory, Weill Cornell Medical College, 4Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medical College For some patients, the only option for fertility preservation is cryopreservation of ovarian tissue. Unfortunately, delayed revascularization undermines follicular viability. Here, we present a protocol to co-transplant human ovarian tissue with endothelial cells for utilization as a cell-based strategy combining accelerated perfusion with a direct paracrine delivery of bioactive molecules.
Other articles by Richard Bodine on PubMed
Engineered Endothelium Provides Angiogenic and Paracrine Stimulus to Grafted Human Ovarian Tissue Scientific Reports. Aug, 2017 | Pubmed ID: 28811567 Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.