Robert J. Crouch Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Biography Publications Institution JoVE Articles Robert J. Crouch has not added a biography. If you are Robert J. Crouch and would like to personalize this page please email our Author Liaison for assistance. Publications High Density of Unrepaired Genomic Ribonucleotides Leads to Topoisomerase 1-mediated Severe Growth Defects in Absence of Ribonucleotide Reductase Nucleic Acids Research. 05, 2020 | Pubmed ID: 32187369 Val143 of Human Ribonuclease H2 is Not Critical For, but Plays a Role in Determining Catalytic Activity and Substrate Specificity PloS One. 2020 | Pubmed ID: 32069311 RNase H2-RED Carpets the Path to Eukaryotic RNase H2 Functions DNA Repair. 12, 2019 | Pubmed ID: 31761672 RNases H: Multiple Roles in Maintaining Genome Integrity DNA Repair. 12, 2019 | Pubmed ID: 31708455 Characterization of Six Recombinant Human RNase H2 Bearing Aicardi-Goutiéres Syndrome Causing Mutations Journal of Biochemistry. Dec, 2019 | Pubmed ID: 31529068 Unlike the Counterpart, Archaeal RNase HII Cannot Process Ribose Monophosphate Abasic Sites and Oxidized Ribonucleotides Embedded in DNA The Journal of Biological Chemistry. 08, 2019 | Pubmed ID: 31300556 Aicardi-Goutières Syndrome Gene Rnaseh2c is a Metastasis Susceptibility Gene in Breast Cancer PLoS Genetics. 05, 2019 | Pubmed ID: 31125342 Two RNase H2 Mutants with Differential RNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development Cell Reports. 10, 2018 | Pubmed ID: 30380406 Abasic and Oxidized Ribonucleotides Embedded in DNA Are Processed by Human APE1 and Not by RNase H2 Nucleic Acids Research. Nov, 2017 | Pubmed ID: 28977421 Effects of Neutral Salts and PH on the Activity and Stability of Human RNase H2 Journal of Biochemistry. Sep, 2017 | Pubmed ID: 28402412 R-Loop Depletion by Over-expressed RNase H1 in Mouse B Cells Increases Activation-Induced Deaminase Access to the Transcribed Strand Without Altering Frequency of Isotype Switching Journal of Molecular Biology. 10, 2017 | Pubmed ID: 28065739 Pathological Ribonuclease H1 Causes R-loop Depletion and Aberrant DNA Segregation in Mitochondria Proceedings of the National Academy of Sciences of the United States of America. 07, 2016 | Pubmed ID: 27402764 The Balancing Act of Ribonucleotides in DNA Trends in Biochemical Sciences. 05, 2016 | Pubmed ID: 26996833 RNase H2 Catalytic Core Aicardi-Goutières Syndrome-related Mutant Invokes CGAS-STING Innate Immune-sensing Pathway in Mice The Journal of Experimental Medicine. Mar, 2016 | Pubmed ID: 26880576 Unlocking the Steric Gate of DNA Polymerase η Leads to Increased Genomic Instability in Saccharomyces Cerevisiae DNA Repair. Nov, 2015 | Pubmed ID: 26340535 Primer Retention Owing to the Absence of RNase H1 is Catastrophic for Mitochondrial DNA Replication Proceedings of the National Academy of Sciences of the United States of America. Jul, 2015 | Pubmed ID: 26162680 Targeted Deletion of the Gene Encoding the La Autoantigen (Sjögren's Syndrome Antigen B) in B Cells or the Frontal Brain Causes Extensive Tissue Loss Molecular and Cellular Biology. Jan, 2014 | Pubmed ID: 24190965 Molecular Biology. A New Twist for Topoisomerase Science (New York, N.Y.). Jun, 2011 | Pubmed ID: 21700860 The Structural and Biochemical Characterization of Human RNase H2 Complex Reveals the Molecular Basis for Substrate Recognition and Aicardi-Goutières Syndrome Defects The Journal of Biological Chemistry. Mar, 2011 | Pubmed ID: 21177858 Crystal Structures of RNase H2 in Complex with Nucleic Acid Reveal the Mechanism of RNA-DNA Junction Recognition and Cleavage Molecular Cell. Nov, 2010 | Pubmed ID: 21095591 An Upstream Open Reading Frame and the Context of the Two AUG Codons Affect the Abundance of Mitochondrial and Nuclear RNase H1 Molecular and Cellular Biology. Nov, 2010 | Pubmed ID: 20823270 Mammalian Mitochondrial DNA Replication Intermediates Are Essentially Duplex but Contain Extensive Tracts of RNA/DNA Hybrid Journal of Molecular Biology. Apr, 2010 | Pubmed ID: 20184890 Ribonuclease H: the Enzymes in Eukaryotes The FEBS Journal. Mar, 2009 | Pubmed ID: 19228196 Specific Recognition of RNA/DNA Hybrid and Enhancement of Human RNase H1 Activity by HBD The EMBO Journal. Apr, 2008 | Pubmed ID: 18337749 Structure of Human RNase H1 Complexed with an RNA/DNA Hybrid: Insight into HIV Reverse Transcription Molecular Cell. Oct, 2007 | Pubmed ID: 17964265 Crystal Structures of RNase H Bound to an RNA/DNA Hybrid: Substrate Specificity and Metal-dependent Catalysis Cell. Jul, 2005 | Pubmed ID: 15989951 Eukaryotic RNases H1 Act Processively by Interactions Through the Duplex RNA-binding Domain Nucleic Acids Research. 2005 | Pubmed ID: 15831789 RNase H2 of Saccharomyces Cerevisiae is a Complex of Three Proteins Nucleic Acids Research. 2004 | Pubmed ID: 14734815 Failure to Produce Mitochondrial DNA Results in Embryonic Lethality in Rnaseh1 Null Mice Molecular Cell. Mar, 2003 | Pubmed ID: 12667461 Multiple Ribonuclease H-encoding Genes in the Caenorhabditis Elegans Genome Contrasts with the Two Typical Ribonuclease H-encoding Genes in the Human Genome Molecular Biology and Evolution. Nov, 2002 | Pubmed ID: 12411600 Quantitative Regulation of Class Switch Recombination by Switch Region Transcription The Journal of Experimental Medicine. Aug, 2001 | Pubmed ID: 11489955 Isolation of RNase H Genes That Are Essential for Growth of Bacillus Subtilis 168 Journal of Bacteriology. Apr, 1999 | Pubmed ID: 10094689 R-Loop Analysis by Dot-Blot Prisila Ramirez1, Robert J. Crouch2, Vivian G. Cheung1,3,4, Christopher Grunseich5 1Life Sciences Institute, University of Michigan, Ann Arbor, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 3Department of Pediatrics, University of Michigan, Ann Arbor, 4Howard Hughes Medical Institute, 5Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health JoVE 62069 Genetics
R-Loop Analysis by Dot-Blot Prisila Ramirez1, Robert J. Crouch2, Vivian G. Cheung1,3,4, Christopher Grunseich5 1Life Sciences Institute, University of Michigan, Ann Arbor, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 3Department of Pediatrics, University of Michigan, Ann Arbor, 4Howard Hughes Medical Institute, 5Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health JoVE 62069 Genetics