Sachdev S. Sidhu Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech University Biography Publications Institution JoVE Articles Sachdev S. Sidhu has not added a biography. If you are Sachdev S. Sidhu and would like to personalize this page please email our Author Liaison for assistance. Publications Construction of Synthetic Antibody Phage-Display Libraries Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 29116499 A General Strategy for Discovery of Inhibitors and Activators of RING and U-box E3 Ligases with Ubiquitin Variants Molecular Cell. Oct, 2017 | Pubmed ID: 29053960 Comprehensive Analysis of the Human SH3 Domain Family Reveals a Wide Variety of Non-canonical Specificities Structure (London, England : 1993). 10, 2017 | Pubmed ID: 28890361 Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10 Journal of Molecular Biology. Nov, 2017 | Pubmed ID: 28587923 Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds Frontiers in Immunology. 2017 | Pubmed ID: 28184223 Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28092036 A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents Journal of Molecular Biology. 01, 2017 | Pubmed ID: 27887869 Engineering Cell Signaling Modulators from Native Protein-protein Interactions Current Opinion in Structural Biology. 08, 2017 | Pubmed ID: 27866084 Saturation Scanning of Ubiquitin Variants Reveals a Common Hot Spot for Binding to USP2 and USP21 Proceedings of the National Academy of Sciences of the United States of America. 08, 2016 | Pubmed ID: 27436899 Inhibition of SCF Ubiquitin Ligases by Engineered Ubiquitin Variants That Target the Cul1 Binding Site on the Skp1-F-box Interface Proceedings of the National Academy of Sciences of the United States of America. Mar, 2016 | Pubmed ID: 26976582 System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes Molecular Cell. Apr, 2016 | Pubmed ID: 26949039 Rapid Isolation of Peptidic Inhibitors of the Solute Carrier Family Transporters OATP1B1 and OATP1B3 by Cell-based Phage Display Selections Biochemical and Biophysical Research Communications. Apr, 2016 | Pubmed ID: 26792727 A Switchable Yeast Display/secretion System Protein Engineering, Design & Selection : PEDS. Oct, 2015 | Pubmed ID: 26333274 A High Through-put Platform for Recombinant Antibodies to Folded Proteins Molecular & Cellular Proteomics : MCP. Oct, 2015 | Pubmed ID: 26290498 Brain Tumor is a Sequence-specific RNA-binding Protein That Directs Maternal MRNA Clearance During the Drosophila Maternal-to-zygotic Transition Genome Biology. May, 2015 | Pubmed ID: 25962635 MicroPET/CT Imaging of Patient-derived Pancreatic Cancer Xenografts Implanted Subcutaneously or Orthotopically in NOD-scid Mice Using (64)Cu-NOTA-panitumumab F(ab')2 Fragments Nuclear Medicine and Biology. Feb, 2015 | Pubmed ID: 25456837 The Cdc15 and Imp2 SH3 Domains Cooperatively Scaffold a Network of Proteins That Redundantly Ensure Efficient Cell Division in Fission Yeast Molecular Biology of the Cell. Jan, 2015 | Pubmed ID: 25428987 The Demonstration of αKlotho Deficiency in Human Chronic Kidney Disease with a Novel Synthetic Antibody Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association. Oct, 2014 | Pubmed ID: 25324355 A Systematic Approach to Identify Novel Cancer Drug Targets Using Machine Learning, Inhibitor Design and High-throughput Screening Genome Medicine. 2014 | Pubmed ID: 25165489 A Structural Portrait of the PDZ Domain Family Journal of Molecular Biology. Oct, 2014 | Pubmed ID: 25158098 Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge ACS Chemical Biology. Oct, 2014 | Pubmed ID: 25140871 Visualization of Arrestin Recruitment by a G-protein-coupled Receptor Nature. Aug, 2014 | Pubmed ID: 25043026 RPRD1A and RPRD1B Are Human RNA Polymerase II C-terminal Domain Scaffolds for Ser5 Dephosphorylation Nature Structural & Molecular Biology. Aug, 2014 | Pubmed ID: 24997600 Alteration of the C-terminal Ligand Specificity of the Erbin PDZ Domain by Allosteric Mutational Effects Journal of Molecular Biology. Oct, 2014 | Pubmed ID: 24813123 Prediction and Experimental Characterization of NsSNPs Altering Human PDZ-binding Motifs PloS One. 2014 | Pubmed ID: 24722214 Synthetic Antibody Technologies Current Opinion in Structural Biology. Feb, 2014 | Pubmed ID: 24721448 Large-scale Interaction Profiling of PDZ Domains Through Proteomic Peptide-phage Display Using Human and Viral Phage Peptidomes Proceedings of the National Academy of Sciences of the United States of America. Feb, 2014 | Pubmed ID: 24550280 Design and Generation of Synthetic Antibody Libraries for Phage Display Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24515463 Peptide Binding Properties of the Three PDZ Domains of Bazooka (Drosophila Par-3) PloS One. 2014 | Pubmed ID: 24466078 Development of Inhibitors in the Ubiquitination Cascade FEBS Letters. Jan, 2014 | Pubmed ID: 24239534 Recombinant Genetic Libraries and Human Monoclonal Antibodies Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24037841 Structural Basis for KIT Receptor Tyrosine Kinase Inhibition by Antibodies Targeting the D4 Membrane-proximal Region Proceedings of the National Academy of Sciences of the United States of America. Oct, 2013 | Pubmed ID: 24127596 Targeting HER2+ Breast Cancer Cells: Lysosomal Accumulation of Anti-HER2 Antibodies is Influenced by Antibody Binding Site and Conjugation to Polymeric Nanoparticles Journal of Controlled Release : Official Journal of the Controlled Release Society. Dec, 2013 | Pubmed ID: 23880472 Engineering Ubiquitin to Modulate the Ubiquitin Proteosome System Cell Cycle (Georgetown, Tex.). Jun, 2013 | Pubmed ID: 23673323 SH3 Interactome Conserves General Function over Specific Form Molecular Systems Biology. Apr, 2013 | Pubmed ID: 23549480 Design of Synthetic Autonomous VH Domain Libraries and Structural Analysis of a VH Domain Bound to Vascular Endothelial Growth Factor Journal of Molecular Biology. Jun, 2013 | Pubmed ID: 23507309 Interaction Domains of Sos1/Grb2 Are Finely Tuned for Cooperative Control of Embryonic Stem Cell Fate Cell. Feb, 2013 | Pubmed ID: 23452850 Engineering and Analysis of Peptide-recognition Domain Specificities by Phage Display and Deep Sequencing Methods in Enzymology. 2013 | Pubmed ID: 23422437 A Strategy for Modulation of Enzymes in the Ubiquitin System Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287719 CDR-H3 Diversity is Not Required for Antigen Recognition by Synthetic Antibodies Journal of Molecular Biology. Feb, 2013 | Pubmed ID: 23219464 A Short DNA Aptamer That Recognizes TNFα and Blocks Its Activity in Vitro ACS Chemical Biology. Jan, 2013 | Pubmed ID: 23046187 KIF14 Negatively Regulates Rap1a-Radil Signaling During Breast Cancer Progression The Journal of Cell Biology. Dec, 2012 | Pubmed ID: 23209302 Immunology: Evolving Superkines for Selectivity Nature Chemical Biology. Dec, 2012 | Pubmed ID: 23183579 Two Synthetic Antibodies That Recognize and Neutralize Distinct Proteolytic Forms of the Ebola Virus Envelope Glycoprotein Chembiochem : a European Journal of Chemical Biology. Nov, 2012 | Pubmed ID: 23111988 Superbinder SH2 Domains Act As Antagonists of Cell Signaling Science Signaling. Sep, 2012 | Pubmed ID: 23012655 Chemical Synthesis and X-ray Structure of a Heterochiral {D-protein Antagonist Plus Vascular Endothelial Growth Factor} Protein Complex by Racemic Crystallography Proceedings of the National Academy of Sciences of the United States of America. Sep, 2012 | Pubmed ID: 22927390 T Cell Receptor-like Recognition of Tumor in Vivo by Synthetic Antibody Fragment PloS One. 2012 | Pubmed ID: 22916301 Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22886261 Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation Science (New York, N.Y.). Sep, 2012 | Pubmed ID: 22878499 Synthetic Antibody Libraries Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22735944 Recombinant Antibodies and in Vitro Selection Technologies Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22723092 Elucidation of the Binding Preferences of Peptide Recognition Modules: SH3 and PDZ Domains FEBS Letters. Aug, 2012 | Pubmed ID: 22691579 Antibodies for All: The Case for Genome-wide Affinity Reagents FEBS Letters. Aug, 2012 | Pubmed ID: 22664378 Synthetic Human Monoclonal Antibodies Toward Staphylococcal Enterotoxin B (SEB) Protective Against Toxic Shock Syndrome The Journal of Biological Chemistry. Jul, 2012 | Pubmed ID: 22645125 Synthetic Antibodies As Tools to Probe RNA-binding Protein Function Molecular BioSystems. Jun, 2012 | Pubmed ID: 22481296 Charged and Hydrophobic Surfaces on the a Chain of Shiga-like Toxin 1 Recognize the C-terminal Domain of Ribosomal Stalk Proteins PloS One. 2012 | Pubmed ID: 22355345 MUSI: an Integrated System for Identifying Multiple Specificity from Very Large Peptide or Nucleic Acid Data Sets Nucleic Acids Research. Mar, 2012 | Pubmed ID: 22210894 Simplified Synthetic Antibody Libraries Methods in Enzymology. 2012 | Pubmed ID: 22208979 Studying Binding Specificities of Peptide Recognition Modules by High-throughput Phage Display Selections Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21877279 Identification of Specificity Determining Residues in Peptide Recognition Domains Using an Information Theoretic Approach Applied to Large-scale Binding Maps BMC Biology. 2011 | Pubmed ID: 21835011 Phage Display of Tissue Inhibitor of Metalloproteinases-2 (TIMP-2): Identification of Selective Inhibitors of Collagenase-1 (metalloproteinase 1 (MMP-1)) The Journal of Biological Chemistry. Sep, 2011 | Pubmed ID: 21715326 The Multiple-specificity Landscape of Modular Peptide Recognition Domains Molecular Systems Biology. Apr, 2011 | Pubmed ID: 21525870 Development of Reagents and Assays for the Detection of Pathogenic Burkholderia Species Faraday Discussions. 2011 | Pubmed ID: 21413172 Evolving Specificity from Variability for Protein Interaction Domains Trends in Biochemical Sciences. Apr, 2011 | Pubmed ID: 21227701 A Portable RNA Sequence Whose Recognition by a Synthetic Antibody Facilitates Structural Determination Nature Structural & Molecular Biology. Jan, 2011 | Pubmed ID: 21151117 Functional Complexes Between YAP2 and ZO-2 Are PDZ Domain-dependent, and Regulate YAP2 Nuclear Localization and Signalling The Biochemical Journal. Dec, 2010 | Pubmed ID: 20868367 Coevolution of PDZ Domain-ligand Interactions Analyzed by High-throughput Phage Display and Deep Sequencing Molecular BioSystems. Oct, 2010 | Pubmed ID: 20714644 Structure of the Complex Between HER2 and an Antibody Paratope Formed by Side Chains from Tryptophan and Serine Journal of Molecular Biology. Sep, 2010 | Pubmed ID: 20654626 MOTIPS: Automated Motif Analysis for Predicting Targets of Modular Protein Domains BMC Bioinformatics. 2010 | Pubmed ID: 20459839 The Functional Capacity of the Natural Amino Acids for Molecular Recognition Molecular BioSystems. Jul, 2010 | Pubmed ID: 20383388 Bayesian Modeling of the Yeast SH3 Domain Interactome Predicts Spatiotemporal Dynamics of Endocytosis Proteins PLoS Biology. Oct, 2009 | Pubmed ID: 19841731 Improving Therapeutic Efficacy of a Complement Receptor by Structure-based Affinity Maturation The Journal of Biological Chemistry. Dec, 2009 | Pubmed ID: 19833734 Rapid Evolution of Functional Complexity in a Domain Family Science Signaling. 2009 | Pubmed ID: 19738200 Crystal Structure of Full-length KcsA in Its Closed Conformation Proceedings of the National Academy of Sciences of the United States of America. Apr, 2009 | Pubmed ID: 19346472 The Importance of Being Tyrosine: Lessons in Molecular Recognition from Minimalist Synthetic Binding Proteins ACS Chemical Biology. May, 2009 | Pubmed ID: 19298050 Inhibition of Wnt Signaling by Dishevelled PDZ Peptides Nature Chemical Biology. Apr, 2009 | Pubmed ID: 19252499 Two-state Selection of Conformation-specific Antibodies Proceedings of the National Academy of Sciences of the United States of America. Mar, 2009 | Pubmed ID: 19208804 A Specificity Map for the PDZ Domain Family PLoS Biology. Sep, 2008 | Pubmed ID: 18828675 Ubiquitin Chain Editing Revealed by Polyubiquitin Linkage-specific Antibodies Cell. Aug, 2008 | Pubmed ID: 18724939 A Dominant Conformational Role for Amino Acid Diversity in Minimalist Protein-protein Interfaces Journal of Molecular Biology. Aug, 2008 | Pubmed ID: 18602117 Submitting Antibodies to Binding Arbitration Nature Chemical Biology. Jun, 2008 | Pubmed ID: 18488004 The Intrinsic Contributions of Tyrosine, Serine, Glycine and Arginine to the Affinity and Specificity of Antibodies Journal of Molecular Biology. Apr, 2008 | Pubmed ID: 18336836 Regulation of a Late Phase of T Cell Polarity and Effector Functions by Crtam Cell. Mar, 2008 | Pubmed ID: 18329370 Synthetic Antibodies for Specific Recognition and Crystallization of Structured RNA Proceedings of the National Academy of Sciences of the United States of America. Jan, 2008 | Pubmed ID: 18162543 Comprehensive Analysis of the Factors Contributing to the Stability and Solubility of Autonomous Human VH Domains The Journal of Biological Chemistry. Feb, 2008 | Pubmed ID: 18045863 Structural and Functional Analysis of the PDZ Domains of Human HtrA1 and HtrA3 Protein Science : a Publication of the Protein Society. Nov, 2007 | Pubmed ID: 17962403 The Type III Effector EspF Coordinates Membrane Trafficking by the Spatiotemporal Activation of Two Eukaryotic Signaling Pathways The Journal of Cell Biology. Sep, 2007 | Pubmed ID: 17893247 Phage Display for Engineering and Analyzing Protein Interaction Interfaces Current Opinion in Structural Biology. Aug, 2007 | Pubmed ID: 17870470 High-throughput Generation of Synthetic Antibodies from Highly Functional Minimalist Phage-displayed Libraries Journal of Molecular Biology. Nov, 2007 | Pubmed ID: 17825836 Structural and Functional Analysis of the Ligand Specificity of the HtrA2/Omi PDZ Domain Protein Science : a Publication of the Protein Society. Aug, 2007 | Pubmed ID: 17656586 Identifying Specificity Profiles for Peptide Recognition Modules from Phage-displayed Peptide Libraries Nature Protocols. 2007 | Pubmed ID: 17545975 Exploring and Designing Protein Function with Restricted Diversity Current Opinion in Chemical Biology. Jun, 2007 | Pubmed ID: 17500026 Full-length Antibodies on Display Nature Biotechnology. May, 2007 | Pubmed ID: 17483836 M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display Methods in Molecular Biology (Clifton, N.J.). 2007 | Pubmed ID: 17041267 Synthetic Therapeutic Antibodies Nature Chemical Biology. Dec, 2006 | Pubmed ID: 17108986 Activation of the Proapoptotic Death Receptor DR5 by Oligomeric Peptide and Antibody Agonists Journal of Molecular Biology. Aug, 2006 | Pubmed ID: 16859704 Comprehensive and Quantitative Mapping of Energy Landscapes for Protein-protein Interactions by Rapid Combinatorial Scanning The Journal of Biological Chemistry. Aug, 2006 | Pubmed ID: 16762925 Comparative Structural Analysis of the Erbin PDZ Domain and the First PDZ Domain of ZO-1. Insights into Determinants of PDZ Domain Specificity The Journal of Biological Chemistry. Aug, 2006 | Pubmed ID: 16737969 Convergent and Divergent Ligand Specificity Among PDZ Domains of the LAP and Zonula Occludens (ZO) Families The Journal of Biological Chemistry. Aug, 2006 | Pubmed ID: 16737968 Tyrosine Plays a Dominant Functional Role in the Paratope of a Synthetic Antibody Derived from a Four Amino Acid Code Journal of Molecular Biology. Mar, 2006 | Pubmed ID: 16413576 Alternative Views of Functional Protein Binding Epitopes Obtained by Combinatorial Shotgun Scanning Mutagenesis Protein Science : a Publication of the Protein Society. Sep, 2005 | Pubmed ID: 16131663 Molecular Recognition by a Binary Code Journal of Molecular Biology. May, 2005 | Pubmed ID: 15854651 A Structure-based Database of Antibody Variable Domain Diversity Journal of Molecular Biology. May, 2005 | Pubmed ID: 15826665 Intramolecular Cooperativity in a Protein Binding Site Assessed by Combinatorial Shotgun Scanning Mutagenesis Journal of Molecular Biology. Apr, 2005 | Pubmed ID: 15755445 Synthetic Antibodies from a Four-amino-acid Code: a Dominant Role for Tyrosine in Antigen Recognition Proceedings of the National Academy of Sciences of the United States of America. Aug, 2004 | Pubmed ID: 15306681 High-affinity Human Antibodies from Phage-displayed Synthetic Fab Libraries with a Single Framework Scaffold Journal of Molecular Biology. Jul, 2004 | Pubmed ID: 15236968 Comprehensive Mutational Analysis of the M13 Major Coat Protein: Improved Scaffolds for C-terminal Phage Display Journal of Molecular Biology. Jul, 2004 | Pubmed ID: 15210356 Phage-displayed Antibody Libraries of Synthetic Heavy Chain Complementarity Determining Regions Journal of Molecular Biology. Apr, 2004 | Pubmed ID: 15066433 Bivalent Antibody Phage Display Mimics Natural Immunoglobulin Journal of Immunological Methods. Jan, 2004 | Pubmed ID: 14736422 Definition of the Consensus Motif Recognized by Gamma-adaptin Ear Domains The Journal of Biological Chemistry. Feb, 2004 | Pubmed ID: 14665628 Comprehensive Mutagenesis of the C-terminal Domain of the M13 Gene-3 Minor Coat Protein: the Requirements for Assembly into the Bacteriophage Particle Journal of Molecular Biology. Sep, 2003 | Pubmed ID: 12972250 Contributions of CDR3 to V H H Domain Stability and the Design of Monobody Scaffolds for Naive Antibody Libraries Journal of Molecular Biology. Sep, 2003 | Pubmed ID: 12963373 The Functional Binding Epitope of a High Affinity Variant of Human Growth Hormone Mapped by Shotgun Alanine-scanning Mutagenesis: Insights into the Mechanisms Responsible for Improved Affinity Journal of Molecular Biology. Sep, 2003 | Pubmed ID: 12946357 Functional Genomics of Intracellular Peptide Recognition Domains with Combinatorial Biology Methods Current Opinion in Chemical Biology. Feb, 2003 | Pubmed ID: 12547433 Exploring Protein-protein Interactions with Phage Display Chembiochem : a European Journal of Chemical Biology. Jan, 2003 | Pubmed ID: 12512072 Origins of PDZ Domain Ligand Specificity. Structure Determination and Mutagenesis of the Erbin PDZ Domain The Journal of Biological Chemistry. Feb, 2003 | Pubmed ID: 12446668 A Minimized M13 Coat Protein Defines the Requirements for Assembly into the Bacteriophage Particle Journal of Molecular Biology. Sep, 2002 | Pubmed ID: 12217696 Comprehensive Functional Maps of the Antigen-binding Site of an Anti-ErbB2 Antibody Obtained with Shotgun Scanning Mutagenesis Journal of Molecular Biology. Jul, 2002 | Pubmed ID: 12079396 Rapid Identification of Small Binding Motifs with High-throughput Phage Display: Discovery of Peptidic Antagonists of IGF-1 Function Chemistry & Biology. Apr, 2002 | Pubmed ID: 11983338 The Erbin PDZ Domain Binds with High Affinity and Specificity to the Carboxyl Termini of Delta-catenin and ARVCF The Journal of Biological Chemistry. Apr, 2002 | Pubmed ID: 11821434 Строительство синтетических Фаговые отображается ВСБ библиотеки с учетом разнообразия Ganggang Huang*1, Zhenwei Zhong*1, Shane Miersch2,3, Sachdev S. Sidhu1,2,3, Shin-chen Hou1, Donghui Wu1 1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto JoVE 57357 Immunology and Infection Масштабируемость Высокая пропускная Выбор фага-дисплее синтетических библиотек антител Shane Miersch1,2, Zhijian Li1,2, Rachel Hanna1,2, Megan E. McLaughlin1,2, Michael Hornsby1,3, Tet Matsuguchi1,3, Marcin Paduch1,4, Annika Sääf1,4, Jim Wells1,3, Shohei Koide1,4, Anthony Kossiakoff1,4, Sachdev S. Sidhu1,2 1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago JoVE 51492 Immunology and Infection
Строительство синтетических Фаговые отображается ВСБ библиотеки с учетом разнообразия Ganggang Huang*1, Zhenwei Zhong*1, Shane Miersch2,3, Sachdev S. Sidhu1,2,3, Shin-chen Hou1, Donghui Wu1 1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto JoVE 57357 Immunology and Infection
Масштабируемость Высокая пропускная Выбор фага-дисплее синтетических библиотек антител Shane Miersch1,2, Zhijian Li1,2, Rachel Hanna1,2, Megan E. McLaughlin1,2, Michael Hornsby1,3, Tet Matsuguchi1,3, Marcin Paduch1,4, Annika Sääf1,4, Jim Wells1,3, Shohei Koide1,4, Anthony Kossiakoff1,4, Sachdev S. Sidhu1,2 1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago JoVE 51492 Immunology and Infection
