Articles by Sachin Ganpat Chavan in JoVE
Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties Youngmin Seo*1, Chanhwi Park*2, Jaewoo Son2, Kyungwoo Lee2, Jangsun Hwang2, Yeonho Jo2, Dohyun Lee2, Muhammad Saad Khan2, Sachin Ganpat Chavan2, Yonghyun Choi2, Dasom Kim2, Assaf A Gilad3, Jonghoon Choi2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.
Other articles by Sachin Ganpat Chavan on PubMed
Label-Free Impedance Sensing of Aflatoxin B₁ with Polyaniline Nanofibers/Au Nanoparticle Electrode Array Sensors (Basel, Switzerland). | Pubmed ID: 29695134 Aflatoxin B (AFB₁) is produced by the and group of fungi which is most hepatotoxic and hepatocarcinogenic and occurs as a contaminant in a variety of foods. AFB₁ is mutagenic, teratogenic, and causes immunosuppression in animals and is mostly found in peanuts, corn, and food grains. Therefore, novel methodologies of sensitive and expedient strategy are often required to detect mycotoxins at the lowest level. Herein, we report an electrochemical impedance sensor that selectively detects AFB₁ at the lowest level by utilizing polyaniline nanofibers (PANI) coated with gold (Au) nanoparticles composite based indium tin oxide (ITO) disk electrodes. The Au-PANI nanocomposites were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) spectroscopy, and electrochemical impedance spectroscopy (EIS). The composite electrode exhibited a 14-fold decrement in |Z| in comparison with the bare electrode. The Au-PANI acted as an effective sensing platform having high surface area, electrochemical conductivity, and biocompatibility which enabled greater loading deposits of capture antibodies. As a result, the presence of AFB₁ was screened with high sensitivity and stability by monitoring the changes in impedance magnitude (|Z|) in the presence of a standard iron probe which was target specific and proportional to logarithmic AFB₁ concentrations (C₁). The sensor exhibits a linear range 0.1 to 100 ng/mL with a detection limit (3) of 0.05 ng/mL and possesses good reproducibility and high selectivity against another fungal mycotoxin, Ochratoxin A (OTA). With regard to the practicability, the proposed sensor was successfully applied to spiked corn samples and proved excellent potential for AFB₁ detection and development of point-of-care (POC) disease sensing applications.