In JoVE (1)
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Articles by Samuel Z. Coffey in JoVE
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae Sheldon G. Watts*1, Justin J. Crowder*1, Samuel Z. Coffey1,2, Eric M. Rubenstein1 1Department of Biology, Ball State University, 2Division of Nephrology, Cincinnati Children's Hospital This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.