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Other Publications (193)
- Neuroscience Letters
- Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
- Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
- Yonsei Medical Journal
- Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions
- Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions
- Anti-cancer Drugs
- Journal of Fluorescence
- Cardiology
- Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions
- The Korean Journal of Internal Medicine
- Cardiology
- Journal of Fluorescence
- Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Journal of Fluorescence
- Acta Oto-laryngologica
- Coronary Artery Disease
- Journal of Fluorescence
- Journal of Fluorescence
- Clinical Cardiology
- Analytica Chimica Acta
- Analytica Chimica Acta
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- The American Journal of Sports Medicine
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
- Luminescence : the Journal of Biological and Chemical Luminescence
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Journal of Fluorescence
- Tumori
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Luminescence : the Journal of Biological and Chemical Luminescence
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
- The Journal of Trauma
- Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
- Clinical Therapeutics
- The Journal of Chemical Physics
- Talanta
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Journal of Shoulder and Elbow Surgery / American Shoulder and Elbow Surgeons ... [et Al.]
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Journal of Fluorescence
- Journal of Fluorescence
- Journal of Fluorescence
- Journal of Molecular Biology
- Angiology
- Angiology
- Luminescence : the Journal of Biological and Chemical Luminescence
- Journal of the Neurological Sciences
- Journal of Fluorescence
- Analytica Chimica Acta
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Luminescence : the Journal of Biological and Chemical Luminescence
- Journal of the American Chemical Society
- Acta Crystallographica. Section E, Structure Reports Online
- International Journal of Cardiology
- Luminescence : the Journal of Biological and Chemical Luminescence
- Journal of Neuroscience Methods
- Coronary Artery Disease
- Clinica Chimica Acta; International Journal of Clinical Chemistry
- Coronary Artery Disease
- The Journal of Chemical Physics
- The American Journal of Sports Medicine
- Clinica Chimica Acta; International Journal of Clinical Chemistry
- Luminescence : the Journal of Biological and Chemical Luminescence
- Journal of Cardiovascular Pharmacology and Therapeutics
- Journal of Cardiac Failure
- Yonsei Medical Journal
- Circulation Journal : Official Journal of the Japanese Circulation Society
- The Canadian Journal of Cardiology
- Physical Chemistry Chemical Physics : PCCP
- Korean Circulation Journal
- Yonsei Medical Journal
- JACC. Cardiovascular Interventions
- Atherosclerosis
- Clinical Cardiology
- Journal of Cardiovascular Pharmacology and Therapeutics
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- JACC. Cardiovascular Interventions
- International Journal of Molecular Medicine
- Korean Journal of Anesthesiology
- Journal of Pediatric Orthopedics
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Korean Circulation Journal
- Acta Cardiologica
- The Journal of Clinical Endocrinology and Metabolism
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- The American Journal of Sports Medicine
- Atherosclerosis
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Atherosclerosis
- The Journal of Chemical Physics
- Clinical Therapeutics
- Clinical and Experimental Hypertension (New York, N.Y. : 1993)
- Angiology
- Atherosclerosis
- Journal of Fluorescence
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Yonsei Medical Journal
- Journal of Fluorescence
- Clinical Cardiology
- Korean Circulation Journal
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Zoological Science
- Luminescence : the Journal of Biological and Chemical Luminescence
- The American Journal of Sports Medicine
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Physical Chemistry Chemical Physics : PCCP
- American Journal of Hypertension
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- The Journal of Clinical Investigation
- Journal of Cardiovascular Pharmacology and Therapeutics
- Luminescence : the Journal of Biological and Chemical Luminescence
- European Heart Journal
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Clinics in Sports Medicine
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Clinica Chimica Acta; International Journal of Clinical Chemistry
- Yonsei Medical Journal
- Journal of Fluorescence
- Skeletal Radiology
- Atherosclerosis
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Korean Circulation Journal
- PloS One
- The Journal of Bone and Joint Surgery. American Volume
- International Journal of Molecular Medicine
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Optics Express
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Hypertension Research : Official Journal of the Japanese Society of Hypertension
- Journal of Nanoscience and Nanotechnology
- Journal of Nanoscience and Nanotechnology
- International Journal of Clinical Pharmacology and Therapeutics
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Analytical and Bioanalytical Chemistry
- Biological Trace Element Research
- Laboratory Animal Research
- Clinics in Orthopedic Surgery
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- International Journal of Cardiology
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Luminescence : the Journal of Biological and Chemical Luminescence
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Journal of Fluorescence
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Biomedical Microdevices
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Luminescence : the Journal of Biological and Chemical Luminescence
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Journal of Magnetic Resonance Imaging : JMRI
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Journal of Human Genetics
- Metabolism: Clinical and Experimental
- Korean Circulation Journal
- Cardiovascular Diabetology
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Hypertension
- Luminescence : the Journal of Biological and Chemical Luminescence
- Journal of Ethnopharmacology
- Knee Surgery & Related Research
- Korean Circulation Journal
- Physical Chemistry Chemical Physics : PCCP
- Diabetes & Metabolism Journal
- Yonsei Medical Journal
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Clinics in Orthopedic Surgery
- Luminescence : the Journal of Biological and Chemical Luminescence
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Antioxidants & Redox Signaling
- The American Journal of Sports Medicine
- Clinica Chimica Acta; International Journal of Clinical Chemistry
- Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
- Yonsei Medical Journal
- Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Archives of Orthopaedic and Trauma Surgery
- PloS One
- Knee Surgery & Related Research
- Oncology Reports
- Archives of Orthopaedic and Trauma Surgery
- International Journal of Cardiology
- Circulation Journal : Official Journal of the Japanese Circulation Society
- Archives of Orthopaedic and Trauma Surgery
- Knee Surgery & Related Research
Articles by Sang Hak Lee in JoVE
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Bir nanometre Hassasiyet Floresans Görüntüleme (Fiona)
Yong Wang*1,2, En Cai*1,2, Janet Sheung1,2, Sang Hak Lee1,2, Kai Wen Teng2,3, Paul R. Selvin1,2,3
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign
Tek flüoroforlar FIONA'sı kullanarak nanometre hassasiyetle lokalize edilebilir. Burada FIONA tekniğin bir özet bilgi ve nasıl Fiona deneyleri tarif edilir.
Other articles by Sang Hak Lee on PubMed
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B-type Natriuretic Peptide Predicts Clinical Presentations and Ventricular Overloading in Patients with Heart Failure
Yonsei Medical Journal.
Aug, 2003 |
Pubmed ID: 12950118 Brain natriuretic peptide (BNP), a neurohormone secreted from the ventricular myocardium in response to hemodynamic load/wall stress, in congestive heart failure (CHF). This study was performed to evaluate the correlation between BNP level and clinical presentations and hemodynamic parameters obtained by echo-Doppler (echo-Doppler) analysis, and its relation with disease severity and ventricular load/wall stress. CHF patients (n=246) were subgrouped by clinical presentations and echo-Doppler findings into 4 groups: diastolic HF only, chronic HF, acute HF, and chronic HF with acute exacerbation. A BNP level of 81.2 pg/ml showed a sensitivity/ specificity of 53.3%/98.4% for detecting CHF (AUC, 0.882; p < 0.0001), and was found to be closely related with the NYHA classification (p < 0.0001). Log BNP was related with LVEF (r2=0.3015, p < 0.0001) and the Meridional wall stress index (r2=0.4052, p < 0.0001). The difference between the BNP levels of the subgroups and BNP control was significant (p < 0.0001), except between the HF group and the controls; control (n=114, 20.9 +/- 31.4 pg/ml), only diastolic HF (n=84, 89.8 +/- 117.6 pg/ml), chronic HF (n=60, 208.2 +/- 210.2 pg/ml), acute HF (n=28, 477.9 +/- 498.4 pg/ml), chronic HF with acute exacerbation (n= 74, 754.1 +/- 419.2 pg/ml). The BNP level was significantly higher in the only diastolic HF group than in the asymptomatic control group with diastolic dysfunction (89.8 +/- 12.8 vs. 22.8 +/- 5.1 pg/ml, p < 0.0001). BNP may be a good indicator for the differential diagnosis of a broad spectrum of heart failures. And, elevated BNP might help to diagnose diastolic HF in patients with diastolic dysfunction.
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Overlapping Vs. One Long Stenting in Long Coronary Lesions
Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions.
Jul, 2004 |
Pubmed ID: 15224294 Intervention of long coronary lesions remains problematic, and optimal treatment strategy is yet to be determined. Despite advancement of stent technology, data are few regarding the efficacy of overlapping stents vs. a single long stent in long coronary lesions. This study was performed to evaluate the results of those strategies for long coronary lesions and to determine the predictors of in-stent restenosis (ISR). Sixty-four lesions (> 20 mm) in 64 patients were treated with either one long stent (group 1, n = 32) or two overlapping stents (group 2, n = 32). Overlapping stents were used at tortuous or calcified lesions and at lesions with diameter discrepancy or significant dissection. Immediate results, follow-up clinical and angiographic outcomes, and predictors of ISR were evaluated. Procedures were successful in all patients in both groups. Clinical and angiographic follow-ups were performed in 54 (84%) cases and 50 (78%) cases, respectively. During the follow-up, major adverse cardiac event occurred in 36% of group 1 and 29% of group 2 (P = 0.56). Six-month ISR rates were 39% in group 1 and 41% in group 2 (P = 0.91). Age (>/= 65 years old) was an independent risk factor of ISR (54% vs. 23%; OR = 4.4; P = 0.04), and distal reference diameter (RD) of less than 2.5 mm tended to predict ISR in multivariate analysis (60% vs. 25%; OR = 3.5; P = 0.06). In conclusion, stent overlapping can be used with outcome similar to that of one long stent in long coronary lesions. The optimal result may be obtained by considering the patient's age and the distal vessel diameter of the lesion.
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Synergistic Interaction Between Gefitinib (Iressa, ZD1839) and Paclitaxel Against Human Gastric Carcinoma Cells
Anti-cancer Drugs.
Sep, 2004 |
Pubmed ID: 15494644 We have evaluated the antitumor effects of gefitinib (Iressa, ZD1839) in SNU-1 human gastric cancer cells (hMLH1-deficient and epidermal growth factor receptor-overexpressed) when given alone or as a doublet with oxaliplatin (LOHP), 5-fluorouracil (5-FU) or paclitaxel (PTX). The four drugs showed IC50s ranging from 1.81 nM to 13.2 microM. LOHP and PTX induced G2/M arrest, 5-FU increased S phase, and gefitinib increased G1 in a concentration-dependent manner. The analysis using the previously developed cytostatic TPi model showed that 64 and 80% of the overall growth inhibition was attributed to cell cycle arrest in cells exposed to 7.55 microM of LOHP or 10 nM of PTX for 72 h, respectively. PTX + gefitinib showed greatest synergism as determined by combination index analysis and apoptosis induced by PTX was potentiated by the co-administration of gefitinib. LOHP + gefitinib showed a similar, although to a lesser degree, synergistic effect. This study demonstrates the antitumor activity and the significant cell cycle arrest induced by gefitinib in SNU-1 human gastric carcinoma cells, and its synergistic interaction with LOHP and PTX.
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Exercise Intolerance in Patients with Atrial Fibrillation: Clinical and Echocardiographic Determinants of Exercise Capacity
Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography.
Dec, 2005 |
Pubmed ID: 16376765 Although exercise intolerance is a major symptom of patients with atrial fibrillation (AF), the factors limiting these patients' exercise capacity remains uncertain. This study evaluated the correlation of clinical and echocardiographic parameters with exercise capacity of patients with AF. In all, 73 patients (61 men and 12 women; mean age 61 years) with chronic AF were included in this study. Those patients with primary valvular diseases were excluded. Standard 2-dimensional and Doppler echocardiography was performed, and we averaged 10 consecutive measurements of each variable. Patients then underwent a symptom-limited treadmill exercise testing. We also measured patients' plasma levels of B-type natriuretic peptide before exercise testing. Of all clinical and echocardiographic parameters we assessed, age (r = -0.45, P = .006), ratio of early mitral inflow velocity to mitral annular velocity (r = -0.35, P = .032), and baseline heart rate were independent predictors of exercise capacity on multivariate regression analysis. In conclusion, patient's age, averaged ratio of early mitral inflow velocity to mitral annular velocity, and baseline heart rate provided useful information on exercise intolerance for patients with AF. Ratio of early mitral inflow velocity to mitral annular velocity, a noninvasive tool for estimating left ventricular filling pressure, may especially have important value for predicting functional capacity in this population as it has in individuals with in sinus rhythm.
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Fluorimetric Determination of Cerium(IV) with Ascorbic Acid
Journal of Fluorescence.
Jan, 2006 |
Pubmed ID: 16496217 A simple, sensitive, and selective method for the determination of cerium(IV), based on the oxidative reaction between cerium(IV) and ascorbic acid, has been described. The fluorescence comes from Ce(III) at lambda(excitation) 298 nm and lambda(emission) 358 nm, which, in turn, is obtained from the oxidation of ascorbic acid by Ce(IV) in the presence of sulfuric acid. The optimum conditions such as concentrations of ascorbic acid, sulfuric acid media and pH of the buffer solution were investigated. The fluorescent intensity of the system is linear over the range 0.0531 microg/ml to 0.3322 mg/ml Ce(IV) and detection limit and correlation coefficient are 0.0145 microg/ml and R=0.99987, respectively.
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Removal of a Femoral Interference Screw That Migrated Posteriorly, Using a Posterior Trans-septal Portal: a Case Report
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA.
May, 2007 |
Pubmed ID: 17096172 Few cases have been reported in which the femoral interference screw has migrated into the posterior compartment after an ACL reconstruction. It usually requires removal, because it leads usually to mechanical symptom. However, the arthroscopic removal of a screw is a technically demanding procedure, especially in the case of the femoral interference screw that has migrated into the femoral notch (posterior compartment), an intact integrated ACL graft or one that is encapsulated around the screw. We present a case in which a displaced femoral interference screw migrated within the posterior compartment 11 years postoperatively, after the graft had been incorporated at the femoral site. Although it is often technically challenging, through the use of a posterior trans-septal portal, we can successfully remove a displaced femoral interference screw even in the most difficult locations in the posterior compartment.
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Structural and Spectroscopic Properties of Trans-dichlorobis(2,2-dimethyl-1,3-diaminopropane)chromium(III) Chloride
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy.
Nov, 2007 |
Pubmed ID: 17307384 The structure of trans-[CrCl(2)(Me(2)tn)(2)]Cl (Me(2)tn=2,2-dimethylpropane-1,3-diamine) has been determined by a single-crystal X-ray diffraction study at 150K. The analysis reveals that there are two independent Cr(III) complex cations in the structure, one with crystallographic inversion symmetry and the other with two-fold rotation symmetry, which are conformational isomers of each other. In both conformations, the chromium atom adopts a distorted octahedral structure with the four nitrogen atoms of two Me(2)tn ligands occupying the equatorial plane and two chlorine atoms occupying trans-axial positions. The six-membered chelate rings are in stable chair conformations with N-Cr-N angles of 87.03(8) degrees and 88.99(8) degrees . The two chelate rings in the centrosymmetric complex cation 1 are anti, while those in the rotation-symmetric complex cation 2 are in syn conformations. The mean Cr-N and Cr-Cl bond lengths are 2.0922 and 2.3253 A, respectively. The infrared and UV-visible absorption spectra of trans-[CrCl(2)(Me(2)tn)(2)]Cl have also been measured. The resolved band maxima of the electronic d-d spectrum are fitted with a secular determinant for a quartet energy state of the d(3) configuration in a tetragonal field including configurational but neglecting spin-orbit coupling. It is confirmed that the nitrogen atoms of the Me(2)tn ligand have a strong sigma-donor character, but the chloro ligand has weak sigma- and pi-donor properties toward the chromium(III) ion.
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The Influence of Maternal Treadmill Running During Pregnancy on Short-term Memory and Hippocampal Cell Survival in Rat Pups
International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience.
Jun, 2007 |
Pubmed ID: 17434282 Maternal exercise during pregnancy has been suggested to exert the beneficial effects on the brain functions of offspring. In the present study, we attempted to determine the effects of maternal treadmill running during pregnancy on short-term memory ability, hippocampal cell survival, and the expression of brain-derived neurotrophic factor (BDNF) mRNA in rat pups. After confirming pregnancy, the pregnant rats were divided into two groups: the control group and the running group (n = 6 in each group). Beginning on the 15th day of pregnancy, the pregnant rats in the running group were forced to run on a treadmill for 30 min at a mild-intensity, once a day until delivery. After all the pregnant rats had given birth, there were rat pups available for use in this study in each group (n = 15 in each group). The latency of the step-down avoidance task was used in order to evaluate the short-term memory ability of the rat pups on the 28 days after birth. Immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) and reverse transcription-polymerase chain reaction (RT-PCR) for BDNF mRNA were performed to determine hippocampal neurogenesis and hippocampal BDNF mRNA expression of the rat pups on the 29 days after birth, respectively. Our results revealed that maternal running during pregnancy resulted in significant increase in the expression of BDNF mRNA, enhanced hippocampal cell survival, and improved the short-term memory capability of rat pups, as compared to those measured in the control group. The findings of our study provide evidence that maternal running during gestational period may enhance the brain functions of offspring.
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Arthroscopic Cystectomy for Popliteal Cysts Through the Posteromedial Cystic Portal
Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association.
May, 2007 |
Pubmed ID: 17478291 In the treatment of patients with popliteal cysts (Baker's cysts), we generally need to address the intra-articular pathologies, most commonly, medial meniscal tears and concomitant connecting valvular mechanisms responsible for the formation and recurrence of the cyst. We introduce here an arthroscopic technique that can treat the associated intra-articular pathology and correct the valvular mechanism of the capsular fold. The most important step is to locate the opening connection between the joint cavity and the popliteal cyst at the posteromedial compartment. The opening is found at the posteromedial side of the medial head of the gastrocnemius after inferior displacement of the capsular fold, which overlies the opening. It is helpful to rotate the bevel of the arthroscope upward to the 11-, 12-, and 1-o'clock positions for most effective visualization of the capsular fold. After the opening connection of the cyst is found, the capsular fold is resected with basket forceps and a shaver inserted through the posteromedial portal to correct the valvular mechanism. Furthermore, we have developed an additional "posteromedial cystic portal," which is located directly above the popliteal cyst and can be effectively used in cystectomy, especially in treatment of cysts that consist of septa and nodules. Arthroscopic popliteal cyst removal with use of an additional posteromedial cystic portal can be effective in treating a cyst with multiple fibrous septa or membranes.
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Measurement of the Graft Angles for the Anterior Cruciate Ligament Reconstruction with Transtibial Technique Using Postoperative Magnetic Resonance Imaging in Comparative Study
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA.
Nov, 2007 |
Pubmed ID: 17721777 The aims of this study were to quantify the angle and placement of an anterior cruciate ligament (ACL) grafted with a single incision ACL reconstruction technique using postoperative magnetic resonance imaging (MRI), and to compare the results with those with a native ACL. Between February 1996 and May 2004, 96 consecutive patients, who had undergone postoperative MRI of the knee followed by an arthroscopically assisted ACL reconstruction with either a hamstring tendon or bone-patellar tendon-bone (BTB) autograft, were enrolled in this study. The femoral tunnel was drilled using the transtibial technique. The patients were divided into two groups; the hamstring tendon graft group (group H; 50 patients) and the BTB graft group (group B; 46 patients). All the patients including both groups in this study underwent postoperative MRI and were followed up for a minimum of 2 years. The control group (group C) consisted of 50 patients whose meniscus tear had been operated on by arthroscopy and whose ACL was intact. The orientation of the ACL ligament or graft was measured using three different methods: the sagittal ACL angle, the ACL-Blumensaat line angle, and the coronal ACL angle. The mean sagittal ACL angle in group C (58.7 +/- 3.8 degrees ) was significantly lower than in groups H (64.6 +/- 4.2 degrees ) and B (71.3 +/- 6.0 degrees ). The mean ACL-Blumensaat line angle in group C (8.6 +/- 3.6 degrees ) was also significantly lower than in groups H (12.8 +/- 5.2 degrees ) and B (18.0 +/- 5.3 degrees ). The mean coronal ACL angle in group C (65.9 +/- 4.4 degrees ) was lower than that in groups H (73.5 +/- 2.4 degrees ) and B (75.2 +/- 2.9 degrees ). The grafted ACL of the hamstring tendon and BTB grafts on the postoperative MRI showed a significant vertical angle in the coronal and sagittal plane compared with the native ACL. In the sagittal plane, the hamstring tendon graft was positioned more obliquely than the BTB graft, which had a larger angle of the tibial tunnel, in an attempt to prevent a graft-tunnel mismatch. The postoperative MRI study showed that the more horizontally the angle of the tibial tunnel can be placed in a single incision ACL reconstruction, the more efficiently it can produce a graft closer to the native ACL.
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Suturing Complete Radial Tears of the Lateral Meniscus
Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association.
Nov, 2007 |
Pubmed ID: 17986421 Lateral meniscus (LM) with complete radial tear (CRT) is common in patients with acute anterior cruciate ligament (ACL) injury. We have been approximating unstable LM CRT in an all-inside suture fashion. We report 3 cases of complete healing of such a torn meniscus on second-look arthroscopy. We recommend approximation of the displaced CRTs of the LM whenever possible because we expect improved healing with this approach. Surgically, the arthroscope is placed into the anteromedial portal and the suture hook into the anterolateral portal with the knee in figure-of-4 position. A suture hook loaded with Ethilon No. 1 (Ethicon, Somerville, NJ) is introduced into the anterolateral portal, and vertical penetration to a single end of the torn LM is achieved. The Ethilon is pushed out until the far-end limb is almost exhausted. The suture hook is removed from the portal, with caution taken not to pull out the penetrated limb from the meniscus. A universal cannula is introduced, and the leading limb is grasped and pulled out. The Ethilon is used as a shuttle relay and is changed to polydioxanone (PDS) II. The suture hook is reintroduced, and the other torn end is penetrated in the same fashion. With complete suture to both meniscal sides, 2 suture limb ends are pulled out, and arthroscopic tying (SMC knot) is performed.
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Crystallization and Preliminary X-ray Diffraction Analysis of YbfF, a New Esterase from Escherichia Coli K12
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications.
Dec, 2007 |
Pubmed ID: 18084091 The product of the recently discovered ybfF gene, which belongs to the esterase family, does not show high sequence similarity to other esterases. To provide the molecular background to the enzymatic mechanism of the ybfF esterase, the ybfF protein from Escherichia coli K12 (Ec_ybfF) was cloned, expressed and purified. The Ec_ybfF protein was crystallized from 60% Tacsimate and 0.1 M bis-Tris propane buffer pH 7.0. Diffraction data were collected to 1.10 A resolution using synchrotron radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 66.09, b = 90.71, c = 92.88 A. With two Ec_ybfF molecules in the asymmetric unit, the crystal volume per unit protein weight is 2.17 A(3) Da(-1), corresponding to a solvent content of 42%.
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Electronic and Vibrational Spectroscopy of Cis-beta-[CrCl(2)(1,4,7,11-tetrazaundecane)chromium(III) Perchlorate
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy.
Aug, 2008 |
Pubmed ID: 17904893 The sharp-line absorption spectrum of cis-beta-[CrCl(2)(2,2,3-tet)]ClO(4) (2,2,3-tet: 1,4,7,11-tetraazaundecane) has been measured between 13,000 and 16,000 cm(-1) at 5K. The 77K emission, 298 K infrared and visible spectra have also been measured. The zero-phonon band in the highly resolved absorption spectrum splits into four components. The vibrational intervals of the electronic ground state were determined from the far-infrared and emission spectra. The 10 electronic bands due to spin-allowed and spin-forbidden transitions were assigned. Using the observed transitions, a ligand field analysis has been performed to probe the ligand field properties of coordinated atoms in the title chromium(III) complex. The X-ray crystal structure at low-temperature was determined to help in assigning the sharp-line electronic transitions.
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Posterolateral Corner Reconstruction for Posterolateral Rotatory Instability Combined with Posterior Cruciate Ligament Injuries: Comparison Between Fibular Tunnel and Tibial Tunnel Techniques
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA.
Mar, 2008 |
Pubmed ID: 18183366 The aim of this study was to compare the results of two different methods of posterolateral corner reconstruction (fibular head tunnel versus tibial tunnel), performed at the same time as a posterior cruciate ligament reconstruction. Between January 1999 and October 2003, 47 patients underwent tensioning of a remnant posterior cruciate ligament and anterolateral bundle reconstruction along with a posterolateral corner reconstruction using a fibular head bone or tibial bone tunnel. Thirty-nine patients determined to be eligible were enrolled in this retrospective study after a minimum follow-up duration of 2 years. The average duration of follow-up was 35.3 months (range 24-70 months). Satisfactory results were achieved in 32 patients (82%) according to the International Knee Documentation Committee(IKDC) scores, and 29 patients (74%) had restored external rotational stability. The fibular head tunnel was superior to the tibial tunnel method in terms of operation time (43.0 +/- 15.7 vs. 66.6 +/- 9.4 min, respectively, P < 0.001) and improved rotational stability (85 vs. 65%, respectively, P = 0.007). However, there were no significant differences seen in anteroposterior stability and clinical assessments [Orthopädische Arbeitsgruppe Knie (OAK) P = 0.277 and IKDC scores P = 0.564]. In grade 2 chronic posterolateral rotatory instability with little or no varus instability associated with injury to the posterior cruciate ligament, a posterolateral reconstructive procedure with a single sling through the fibular tunnel offers advantages of less surgical morbidity and operation time, as well as better rotational stability, over reconstruction through the tibial tunnel.
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High-resolution Structure of YbfF from Escherichia Coli K12: a Unique Substrate-binding Crevice Generated by Domain Arrangement
Journal of Molecular Biology.
Mar, 2008 |
Pubmed ID: 18215690 Esterases are one of the most common enzymes and are involved in diverse cellular functions. ybfF protein from Escherichia coli (Ec_ybfF) belongs to the esterase family for the large substrates, palmitoyl coenzyme A and malonyl coenzyme A, which are important cellular intermediates for energy conversion and biomolecular synthesis. To obtain molecular information on ybfF esterase, which is found in a wide range of microorganisms, we elucidated the crystal structures of Ec_ybfF in complexes with small molecules at resolutions of 1.1 and 1.68 A, respectively. The structure of Ec_ybfF is composed of a globular alpha/beta hydrolase domain with a three-helical bundle cap, which is linked by a kinked helix to the alpha/beta hydrolase domain. It contains a catalytic tetrad of Ser-His-Asp-Ser with the first Ser acting as a nucleophile. The unique spatial arrangement and orientation of the helical cap with respect to the alpha/beta hydrolase domain form a substrate-binding crevice for large substrates. The helical cap is also directly involved in catalysis by providing a substrate anchor, viz., the conserved residues of Arg123 and Tyr208. The high-resolution structure of Ec_ybfF shows that the inserted helical bundle structure and its spatial orientation with respect to the alpha/beta hydrolase domain are critical for creating a large inner space and constituting a specific active site, thereby providing the broad substrate spectrum toward large biomolecules.
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Effects of Atrial Fibrillation on Arterial Stiffness in Patients with Hypertension
Angiology.
Aug-Sep, 2008 |
Pubmed ID: 18388027 Arterial stiffness is significantly correlated with cardiovascular risk in patients with hypertension. Although arterial stiffness increases with age and other vascular risk factors, the effect of heart rhythm on arterial stiffness is uncertain. The aim of this study was to determine whether the presence of atrial fibrillation is associated with arterial stiffness, as determined by heart to femoral pulse wave velocity, in patients with hypertension. Heart to femoral pulse wave velocity was measured, and comprehensive transthoracic echocardiography was performed in 68 subjects (35 subjects with sinus rhythm, 33 subjects with atrial fibrillation). Potential determinants of heart to femoral pulse wave velocity were identified by univariate and multivariate analyses. Individuals with atrial fibrillation had higher heart to femoral pulse wave velocity than those with sinus rhythm (1028 +/- 222 vs 923 +/- 110 cm/s, P = .03). Age, the presence of atrial fibrillation, systolic blood pressure, and pulse pressure were found to be significantly correlated with heart to femoral pulse wave velocity. After adjusting for possible con-founders, multivariate analysis identified systolic blood pressure (P = .003) and the presence of atrial fibrillation (P = .007) as independent determinants of heart to femoral pulse wave velocity. The presence of atrial fibrillation was significantly correlated with a higher pulse wave velocity, independently of age or blood pressure in patients with hypertension.
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Simultaneous Determination of Levodopa and Carbidopa by Synchronous Fluorescence Spectrometry Using Double Scans
Analytica Chimica Acta.
Jun, 2008 |
Pubmed ID: 18539165 A synchronous fluorescence spectrometric method is described for the simultaneous determination of binary mixtures of levodopa and carbidopa in pharmaceutical formulation and urine sample, without prior separation steps, using two scans. At Delta lambda = 30 nm, only carbidopa yields a detectable signal that is independent of the presence of levodopa. Similarly, at Delta lambda = 65 nm the signal of levodopa is not influenced by the presence of carbidopa. Signals at two wavelengths, 288 nm (Delta lambda = 30 nm) and 281 nm (Delta lambda = 65 nm), vary linearly with carbidopa and levodopa concentrations over the range 0.019-1.971 microg mL(-1) (for levodopa) and 0.022-2.262 microg mL(-1) (for carbidopa), respectively. The correlation coefficients for the standard calibration graphs were 0.9962 and 0.9951 (n=10) for carbidopa and levodopa, respectively. The limits of detection (LOD estimated as per IUPAC recommendations) were 0.01 and 0.006 microg mL(-1) for carbidopa and levodopa, respectively. The method was successfully applied to the determination of levodopa and carbidopa in pharmaceutical formulation and urine sample. The recovery results were satisfactory.
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Anion Clusters of Naphthalene and Solvents: Structure, Ion Core, and Intermolecular Interactions
Physical Chemistry Chemical Physics : PCCP.
Nov, 2009 |
Pubmed ID: 19830330 We carried out a comparative study on the anion clusters of naphthalene with various solvents to understand the nature of intermolecular interactions involving an aromatic anion. Photoelectron spectra of mass-selected naphthalene anion clusters, (Np)(1)(-)(Sol)(n) (Sol = benzene, naphthalene, water, and acetone), were obtained, from which the electron affinities were estimated. The electron affinities were significantly different from the vertical detachment energies due to the geometry difference between the neutral and anion clusters along intermolecular coordinates. Theoretical calculations showed that the most stable structure of the naphthalene-acetone anion cluster tends to be a -shaped geometry because the intermolecular interaction is dominated by pi-hydrogen bonding. With the attachment of a second solvent, solvent-solvent interaction was found to compete with ion-solvent interaction for the stable geometries of the anion clusters.
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Effects of Atorvastatin 20 Mg, Rosuvastatin 10 Mg, and Atorvastatin/ezetimibe 5 Mg/5 Mg on Lipoproteins and Glucose Metabolism
Journal of Cardiovascular Pharmacology and Therapeutics.
Jun, 2010 |
Pubmed ID: 20147603 The aim of this study was to compare the effects of 3 different statin regimens that have equivalent low-density lipoprotein cholesterol (LDL-C) lowering efficacy on the apolipoprotein B/A1 ratio and glucose metabolism. After a 4-week dietary lead-in, 90 hypercholeserolemic patients were randomly assigned to 1 of 3 treatment groups for 8 weeks: atorvastatin 20 mg, rosuvastatin 10 mg, or atorvastatin/ezetimibe 5 mg/5 mg. At drug treatment week 8, we compared the percentage changes in lipid parameters, apolipoprotein B/A1 ratio, hemoglobin A1c, and homeostasis model assessment-insulin resistance (HOMA-IR) from baseline. Seventy-six patients completed the study and the percentage changes in LDL-C were comparable among the groups. However, the percentage reduction in the apolipoprotein B/A1 ratio was significantly greater in the rosuvastatin group (-47% +/- 14%, P = .04) and the combination group (-46% +/- 8%, P = .05) than in the atorvastatin group (-39% +/- 11%). The percentage increase in hemoglobin A1c was small but significantly greater in the atorvastatin group compared to the combination group (3.0% +/- 5.2% and -0.4% +/- 4.0%, P = .03). The effect of rosuvastatin on hemoglobin A1c was not different from those of the other 2 regimens. The effects of 3 statin regimens were similar on HOMA-IR. In conclusion, 3 statin regimens have differential effect on apolipoprotein B/A1 and glycemic control after comparable LDL-C reduction.
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Combined Reconstruction for Posterolateral Rotatory Instability with Anterior Cruciate Ligament Injuries of the Knee
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA.
Sep, 2010 |
Pubmed ID: 20182869 If posterolateral rotatory instability (PLRI) injury in patients with a torn anterior cruciate ligament (ACL) is not diagnosed and treated, ACL reconstruction can fail. We retrospectively evaluated the clinical outcome after reconstructions between 2002 and 2007 of both the ACL and the posterolateral corner (PLC) in 44 knees with combined ACL and PLC injuries. The median follow-up duration was 49 months (range, 24-68 months). ACL reconstruction employed autogenous hamstring grafts from the ipsilateral knee. For grade II PLRI, a posterolateral corner sling through the fibular head was placed obliquely from the anteroinferior aspect to the posterosuperior aspect using autogenous hamstring grafts of the contralateral knee. Clinical outcomes were evaluated using the OAK (Orthopadishe Arbeitsgruppe Knie) and IKDC (International Knee Documentation Committee) knee scoring systems. Anterior stability was measured on pull stress radiographs using a Telos stress device and the manual maximum displacement test using a KT-1000 arthrometer with the knee flexed 30 degrees. PLRI was classified according to varus and rotational instability preoperatively and at final follow-up. Median OAK scores improved from 71 points (range, 48-86) to 93 points (range, 75-100). Satisfactory IKDC results were achieved in 39 knees (89%). As for anterior stability, as measured by anterior stress radiography, mean side-to-side displacement difference dropped significantly from 6.9 +/- 1.9 preoperatively to 1.4 +/- 1.1 mm at final follow-up. Forty patients (91%) had the same or better rotational stability compared to the normal side. Varus stress radiographs showed mean side-to-side displacement differences dropped from 1.8 +/- 1.7 preoperatively to 0.4 +/- 0.8 mm at final follow-up. Thus, chronic ACL deficiency is often accompanied by grade II PLRI and can be treated successfully by arthroscopic ACL reconstruction paired with posterolateral reconstruction employing a single sling through the fibular tunnel and a hamstring tendon autograft.
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Comparison of Effects of Two Different Formulations of Clopidogrel Bisulfate Tablets on Platelet Aggregation and Bleeding Time in Healthy Korean Volunteers: A Single-dose, Randomized, Open-label, 1-week, Two-period, Phase IV Crossover Study
Clinical Therapeutics.
Aug, 2010 |
Pubmed ID: 20974324 Clopidogel bisulfate, an oral antiplatelet agent that works by inhibiting adenosine diphosphate-induced platelet aggregation, is used in the treatment of coronary artery disease, peripheral vascular disease, and cerebrovascular disease. The newly developed generic version of Clopidogrel bisulfate has a mechanism of action comparable to the reference formulation.
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Left Ventricular Hypertrophy Determines the Severity of Diastolic Dysfunction in Patients with Nonvalvular Atrial Fibrillation and Preserved Left Ventricular Systolic Function
Clinical and Experimental Hypertension (New York, N.Y. : 1993).
2010 |
Pubmed ID: 21091364 Regression of left ventricular (LV) hypertrophy (LVH) is known to be related to a lower incidence of stroke in hypertensive patients with nonvalvular atrial fibrillation (NV-AF). However, its mechanism remains controversial. Recently, diastolic dysfunction (DD) was reported to be correlated with ischemic stroke in NV-AF. We hypothesized that hypertension (HTN) and resultant LVH might be associated with the severity of DD in NV-AF. Two hundred and ninety-four patients (204 males, age 66 ± 12 y) with NV-AF with preserved LV systolic function were included. Clinical and echocardiographic data were compared between patients with enlarged left atrial (LA) volume (n = 237) and patients with normal LA. Age (60 ± 12 vs. 67 ± 11 years), sex (male; 81 vs. 62%), duration of NV-AF (4.1 ± 7.8 vs. 45.7 ± 49.0 months), brain natriuretic peptide (108.3 ± 129.3 vs. 236.1 ± 197.0 pg/mL), right ventricular systolic pressure (24.5 ± 5.5 vs. 33.1 ± 11.1 mmHg), mitral inflow velocity (E [77.4 ± 22.2 vs. 88.3 ± 22.0 cm/s]), LV mass index (LVMI [87.6 ± 22.2 vs. 105.1 ± 23.2 g/m(2)]), peak systolic mitral annular velocity (S' [7.2 ± 2.0 vs. 5.8 ± 1.8 cm/s]), and mitral inflow velocity to diastolic mitral annular velocity (E/E' [9.8 ± 3.4 vs. 12.1 ± 4.4]) were significantly different between the two groups, respectively (P < 0.05). In multivariate analysis, LVMI was independently correlated with increased LA volume (OR: 1.037 [95% CI: 1.011-1.063], P < 0.05), whereas HTN was not. LA enlargement, which reflects the severity and chronicity of DD, is independently associated with LVH in patients with NV-AF. Therefore, regression of LVH with anti-hypertensive treatment may lead to improvement of diastolic function and favorable clinical outcomes in hypertensive patients with NV-AF.
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Genomic Structure and Expression Analyses of the PYGM Gene in the Thoroughbred Horse
Zoological Science.
Apr, 2011 |
Pubmed ID: 21466345 Muscle glycogen Phosphorylase (PYGM) has been shown to catalyze the degradation of glycogen to glucose-1-phosphate. The PYGM gene can contribute to providing energy to the body by disassembling the glycogen in muscle. Here, we analyzed the genomic structure and expression of the PYGM gene in the thoroughbred horse. The PYGM gene, containing several transposable elements (MIRs, LINEs, and MERs), was highly conserved in mammalian genomes. In order to understand the expression of the horse PYGM gene, we performed quantitative RT-PCR using 11 thoroughbred horse tissue samples. The horse PYGM gene was broadly expressed in all tissues tested. In particular, the highest expression of the horse PYGM gene was observed in skeletal muscle tissue relative to the other tissues. Interestingly, the horse PYGM gene contains fewer mobile elements than its human ortholog, resulting in an increase in the structural stability of the PYGM gene sequence. This study provides insights into the genomic structure of the horse PYGM gene that may be useful in future studies of its association with exercise capability.
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In Vivo Visualization and Attenuation of Oxidized Lipid Accumulation in Hypercholesterolemic Zebrafish
The Journal of Clinical Investigation.
Dec, 2011 |
Pubmed ID: 22105168 Oxidative modification of LDL is an early pathological event in the development of atherosclerosis. Oxidation events such as malondialdehyde (MDA) formation may produce specific, immunogenic epitopes. Indeed, antibodies to MDA-derived epitopes are widely used in atherosclerosis research and have been demonstrated to enable cardiovascular imaging. In this study, we engineered a transgenic zebrafish with temperature-inducible expression of an EGFP-labeled single-chain human monoclonal antibody, IK17, which binds to MDA-LDL, and used optically transparent zebrafish larvae for imaging studies. Feeding a high-cholesterol diet (HCD) supplemented with a red fluorescent lipid marker to the transgenic zebrafish resulted in vascular lipid accumulation, quantified in live animals using confocal microscopy. After heat shock-induced expression of IK17-EGFP, we measured the time course of vascular accumulation of IK17-specific MDA epitopes. Treatment with either an antioxidant or a regression diet resulted in reduced IK17 binding to vascular lesions. Interestingly, homogenates of IK17-EGFP-expressing larvae bound to MDA-LDL and inhibited MDA-LDL binding to macrophages. Moreover, sustained expression of IK17-EGFP effectively prevented HCD-induced lipid accumulation in the vascular wall, suggesting that the antibody itself may have therapeutic effects. Thus, we conclude that HCD-fed zebrafish larvae with conditional expression of EGFP-labeled oxidation-specific antibodies afford an efficient method of testing dietary and/or other therapeutic antioxidant strategies that may ultimately be applied to humans.
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A Terbium-sensitized Spectrofluorimetric Method for Determination of Catecholamines in a Serum Sample with Micelle Medium
Luminescence : the Journal of Biological and Chemical Luminescence.
Jan-Feb, 2012 |
Pubmed ID: 21692167 A terbium-sensitized spectrofluorimetric method has been developed for determination of catecholamines such as norepinephrine (NE), epinephrine (EP) and dopamine (DA), using sodium dodecyl benzene sulphonate (SDBS). Fluorescence sensitization of terbium ions (Tb(3+) ) by complexation with catecholamines in the presence of SDBS was observed. The fluorescence intensities of the Tb(3+) -catecholamine complexes were highly enhanced by introducing SDBS with an emission maximum at 545 nm after excitation at 290 nm. The conditions for the complex formation of Tb(3+) -catecholamine were investigated systematically and optimized to determine catecholamines in a serum sample. Under the optimum conditions, the fluorescence intensities of the Tb(3+) -catecholamine complexes were increased linearly with the concentration of NE, EP and DA over the ranges 2.5 × 10(-10) -1.0 × 10(-8) , 2.5 × 10(-10) -1.0 × 10(-8) and 2.5 × 10(-9) -1.0 × 10(-7) g/mL with correlation coefficients of 0.999, 0.999 and 0.9996, respectively. The limits of detection (3δ) of NE, EP and DA were found to be 4.6 × 10(-11) , 7.8 × 10(-11) and 8.38 × 10(-10) g/mL, respectively. Precision of the method was tested at the concentration level of 1.2 × 10(-7) g/mL for five replicate measurements of NE, EP and DA, giving relative standard deviations (RSDs) of 1.41%, 1.23% and 1.89%, respectively. The interaction mechanism of the Tb(3+) -catecholamine complexes system was investigated and presented with ultraviolet absorption spectra. The proposed method has been applied for the quantitative determination of NE, EP and DA in a spiked serum sample and a pharmaceutical preparation sample.
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Palmitate Promotes the Paracrine Effects of Macrophages on Vascular Smooth Muscle Cells: the Role of Bone Morphogenetic Proteins
PloS One.
2012 |
Pubmed ID: 22363399 Saturated fatty acids are known to activate macrophages and induce vascular inflammation. Although cytokines from activated macrophage influence other vascular cells, the influence of saturated fatty acids on the paracrine effect of macrophages is not fully understood yet. Here we examined the impact of palmitate on the effect of macrophages on vascular smooth muscle cells (SMCs) and their mediators. SMCs proliferation increased significantly after treatment with conditioned media from palmitate-stimulated RAW264.7 cells. SMC migration was found to be greater after treatment with palmitate-conditioned media. SM α-actin and SM22α were decreased in SMCs treated with palmitate-conditioned media. When stimulated with palmitate, RAW264.7 cells secreted more bone morphogenetic protein (BMP)2 and BMP4 into the cell culture media. SMC proliferation, migration, and phenotypic changes were attenuated after treatment of neutralizing antibodies against BMPs or knockdown of BMPs with siRNA. The influences of these proteins were further confirmed by direct treatment of recombinant BMP2 and BMP4 on SMCs. Particularly, the effects of BMPs on SMC migration on phenotypic change were obvious, whereas their effect on SMC proliferation seemed not significant or modest. In conclusion, palmitate promoted macrophages' paracrine effects on SMC proliferation, migration, and phenotypic change. The effect of stimulated macrophages was mediated, at least in part, by BMP2 and BMP4. These results suggest a novel mechanism linking saturated fatty acids and the progression of vascular diseases that is possibly mediated by BMPs from macrophages.
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UV Radiation-induced Skin Aging in Hairless Mice is Effectively Prevented by Oral Intake of Sea Buckthorn (Hippophae Rhamnoides L.) Fruit Blend for 6 Weeks Through MMP Suppression and Increase of SOD Activity
International Journal of Molecular Medicine.
Aug, 2012 |
Pubmed ID: 22641502 Oxidative stress and oxidative photodamage induced by UV radiation can cause serious skin damage that is characterized by wrinkling, roughness, laxity and pigmentation. The effects of a sea buckthorn (Hippophae rhamnoides L.) fruit blend (SFB) containing sea buckthorn fruit extract, blueberry extract and collagen on UV-induced skin aging were examined by treating hairless mice for 6 weeks with UV irradiation and SFB administered orally. The effects of SFB were measured in the skin of these mice by phenotypical and histological analysis and western blotting. According to wrinkle formation analysis, the oral intake of SFB induced a decrease in wrinkle formation in the damaged skin of UV-irradiated mice. The thickness of the epidermis and dermis in the vitamin extracts (Vit)- and SFB-treated group was lower than that in the vehicle-treated group, but the group treated with SFB50 was the most effective group. The mice treated with the Vit- or SFB solution maintained a normal moisture content through the inhibition of transdermal water loss (TEWL) and an increase in skin moisture content. Furthermore, the levels of matrix metalloproteinase (MMP) and collagen protein expression were assessed in five groups to examine the mechanisms underlying the effects of SFB oral intake. The application of SFB induced a decrease in MMP-1 and -9 expression to the levels observed in the vehicle-treated group, but MMP-9 expression showed a much larger decrease than MMP-1. Furthermore, the expression of collagen-1 in the skin corresponded to MMP expression except for the SFB30-treated group, whereas the superoxide dismutase (SOD) activity was increased dramatically in the SFB50-treated group. These results suggest that SFB has potential as a protective and therapeutic drug candidate against skin aging that functions by regulating the moisture content, MMP expression levels and SOD activity.
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Using Fixed Fiduciary Markers for Stage Drift Correction
Optics Express.
May, 2012 |
Pubmed ID: 22714205 To measure nanometric features with super-resolution requires that the stage, which holds the sample, be stable to nanometric precision. Herein we introduce a new method that uses conventional equipment, is low cost, and does not require intensive computation. Fiduciary markers of approximately 1 µm x 1 µm x 1 µm in x, y, and z dimensions are placed at regular intervals on the coverslip. These fiduciary markers are easy to put down, are completely stationary with respect to the coverslip, are bio-compatible, and do not interfere with fluorescence or intensity measurements. As the coverslip undergoes drift (or is purposely moved), the x-y center of the fiduciary markers can be readily tracked to 1 nanometer using a Gaussian fit. By focusing the light slightly out-of-focus, the z-axis can also be tracked to < 5 nm for dry samples and
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Comparison of Arterial Stiffness Indices Measured by the Colins and SphygmoCor Systems
Hypertension Research : Official Journal of the Japanese Society of Hypertension.
Dec, 2012 |
Pubmed ID: 22833159 Arterial stiffness is a known independent predictor of cardiovascular mortality. The Colins system is an easy device and has gained widespread use, but the cutoff value for high-risk central arterial stiffness is not well established. We investigated the correlation between arterial stiffness measured by the Colins system with conventional measurements from the SphygmoCor system. Arterial pulse wave velocity (PWV) and augmentation indices (AIs) were measured on a single visit using two different devices in 948 patients with hypertension or coronary artery disease. Strong positive correlations were observed for PWV values measured by the SphygmoCor and Colins systems. The Colins system measurements accurately predicted high-risk central arterial stiffness, defined as carotid-femoral PWV≥12 m s(-1), with an area under the receiver-operating characteristic curve (AUC) of 0.884 (heart-femoral PWV, hfPWV) and 0.830 (brachial-ankle PWV, baPWV) in the training set (N=664). The cutoff values, 11.18 (hfPWV) and 16.17 m s(-1) (baPWV), showed good discrimination in the validation set (N=284), with sensitivity of 83.3 (hfPWV) and 76.0% (baPWV), and specificity of 74.9 (hfPWV) and 82.6% (baPWV). The SphygmoCor and Colins AI systems also showed moderate positive correlation. The Colins AI system better predicted high-risk central pulse pressure as defined by pulse pressure≥50 mm Hg (AUC: Colins, 0.765; SphygmoCor, 0.692; P=0.011). Arterial stiffness measured by the Colins system showed strong positive correlation and agreement with the SphygmoCor system measurement. Cutoff values for high-risk central arterial stiffness in the Colins system need further validation in a prospective study.
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Enzymeless Determination of Total Sugar by Luminol-tetrachloroaurate Chemiluminescence on Chip to Analyze Food Samples
Analytical and Bioanalytical Chemistry.
Dec, 2012 |
Pubmed ID: 23052873 Chemiluminescence (CL) emission from luminol-tetrachloroaurate ([AuCl(4)](-)) system studied in presence of monosaccharide sugars such as glucose and fructose was investigated on a microfluidic chip fabricated by the soft lithography technique. CL emission from the luminol-[AuCl(4)](-) system at 430 nm was intensified remarkably by the catalytic activity of glucose and fructose at room temperature. Under optimized conditions, the CL emission intensity of the system was found to be linearly related to the concentration of the sugars. Based on this observation, nonenzymatic determination of total sugar (glucose, fructose, or hydrolyzable sucrose) was performed in a rapid and sensitive analytical method. The results revealed that the linearity ranged from 9 to 1,750 μM for glucose and 80 to 1,750 μM for fructose, with a limit of detection of 0.65 and 0.69 μM, respectively. The relative standard deviations determined at 250 μM based on six repetitive injections were 1.13 and 1.15% for glucose and fructose, respectively. The developed method was successfully applied for determination of the total sugar concentration in food and beverages.
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Selenium Significantly Inhibits Adipocyte Hypertrophy and Abdominal Fat Accumulation in OLETF Rats Via Induction of Fatty Acid β-oxidation
Biological Trace Element Research.
Dec, 2012 |
Pubmed ID: 23076603 A combination of selenium (Se) with other trace element is associated with partially modulate fatty acid distribution as well as reduction of the body weight and feed efficiency. To investigate whether or not Se treatment has an impact on lipid metabolism, we examined the levels of lipid metabolism-related factors, including abdominal fat, adiponectin, cholesterol, very long chain dehydrogenase (VLCAD), and medium chain acyl-CoA dehydrogenase (MCAD) in 20-week-old Otsuka Long-Evans Tokushima Fatty (OLETF) rats following sodium selenite treatment for 2 weeks. Herein, we observed that (a) Se treatment induced insulin-like effects by lowering the serum glucose level in rats; (b) Se-treated rats showed significance values decreases in abdominal fat mass, adipocyte size, and adiponectin, which are associated with lipid metabolism; (c) Se treatment led to reduced levels of cholesterol, triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol; (d) fat tissue in Se-treated rats displayed significantly lower expression of adipocyte marker genes along with increased expression of VLCAD and MCAD; and (e) fatty liver formation and β-oxidation gene expression were both significantly reduced in liver tissue of Se-treated rats. Therefore, our results suggest that Se may induce inhibition of adipocyte hypertrophy and abdominal fat accumulation along with suppression of fatty liver formation by the differential regulation of the gene expression for fatty acid β-oxidation in the OLETF model.
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Aqueous Extract of Liriope Platyphylla, a Traditional Chinese Medicine, Significantly Inhibits Abdominal Fat Accumulation and Improves Glucose Regulation in OLETF Type II Diabetes Model Rats
Laboratory Animal Research.
Sep, 2012 |
Pubmed ID: 23091518 Liriope platyphylla is a medical herb that has long been used in Korea and China to treat cough, sputum, neurodegenerative disorders, obesity, and diabetes. The aims of this study were to determine the antidiabetic and antiobesity effects of aqueous extract of L. platyphylla (AEtLP) through glucose and lipid regulation in both pre-diabetes and obesity stage of type II diabetes model. Two concentrations of AEtLP were orally administrated to OLETF (Otsuka Long-Evans Tokushima Fatty) rats once a day for 2 weeks, after which changes in glucose metabolism and fat accumulation were measured. Abdominal fat mass dramatically decreased in AEtLP-treated OLETF rats, whereas glucose concentration slightly decreased in all AEtLP-treated rats. However, compared to vehicle-treated OLETF rats, only AEtLP10 (10% concentration)-treated OLETF rats displayed significant induction of insulin production, whereas AEtLP5 (5% concentration)-treated OLETF rats showed a lower level of insulin. Although serum adiponectin level increased in only AEtLP5-treated rats, significant alteration of lipid concentration was detected in AEtLP5-treated OLETF rats. Expression of Glut-1 decreased in all AEtLP-treated rats, whereas Akt phosphorylation increased only in AEtLP10-treated OLETF rats. Furthermore, the pattern of Glut-3 expression was very similar with that of Glut-1 expression, which roughly corresponded with the phosphorylation of c-Jun N-teminal kinase (JNK) and p38 in the mitogen-activated protein kinase pathway. Therefore, these findings suggest that AEtLP should be considered as a therapeutic candidate during pre-diabetes and obesity stage capable of inducing insulin secretion from pancreatic β-cells, glucose uptake in liver cells, as well as a decrease in fat and lipid accumulation.
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A Chelate Complex-enhanced Luminol System for Selective Determination of Co(II), Fe(II) and Cr(III)
Luminescence : the Journal of Biological and Chemical Luminescence.
May-Jun, 2013 |
Pubmed ID: 22715144 A determination method for Co(II), Fe(II) and Cr(III) ions by luminol-H2 O2 system using chelating reagents is presented. A metal ion-chelating ligand complex with a Co(II) ion and a chelating reagent like ethylenediaminetetraacetic acid (EDTA) produced highly enhanced chemiluminescence (CL) intensity as well as longer lifetime in the luminol-H2 O2 system compared to metals that exist as free ions. Whereas free Cu(II) and Pb(II) ions had a strong catalytic effect on the luminol-H2 O2 system, significantly, the complexes of Cu(II) and Pb(II) with chelating reagents lost their catalytic activity due to the chelating reagents acting as masking agents. Based on the observed phenomenon, it was possible to determine Co(II), Fe(II) and Cr(III) ions with enhanced sensitivity and selectivity using the chelating reagents of the luminol-H2 O2 system. The effects of ligand, H2 O2 concentration, pH, buffer solution and concentrations of chelating reagents on CL intensity of the luminol-H2 O2 system were investigated and optimized for the determination of Co(II), Fe(II) and Cr(III) ions. Under optimized conditions, the calibration curve of metal ions was linear over the range of 2.0 × 10(-8) to 2.0 × 10(-5) M for Co(II), 1.0 × 10(-7) to 2.0 × 10(-5) M for Fe (II) and 2.0 × 10(-7) to 1.0 × 10(-4) M for Cr(III). Limits of detection (3σ/s) were 1.2 × 10(-8) , 4.0 × 10(-8) and 1.2 × 10(-7) M for Co(II), Fe(II) and Cr(III), respectively.
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A Genome-wide Association Study of a Coronary Artery Disease Risk Variant
Journal of Human Genetics.
Mar, 2013 |
Pubmed ID: 23364394 Although over 30 common genetic susceptibility loci have been identified to be independently associated with coronary artery disease (CAD) risk through genome-wide association studies (GWAS), genetic risk variants reported to date explain only a small fraction of heritability. To identify novel susceptibility variants for CAD and confirm those previously identified in European population, GWAS and a replication study were performed in the Koreans and Japanese. In the discovery stage, we genotyped 2123 cases and 3591 controls with 521 786 SNPs using the Affymetrix SNP Array 6.0 chips in Korean. In the replication, direct genotyping was performed using 3052 cases and 4976 controls from the KItaNagoya Genome study of Japan with 14 selected SNPs. To maximize the coverage of the genome, imputation was performed based on 1000 Genome JPT+CHB and 5.1 million SNPs were retained. CAD association was replicated for three GWAS-identified loci (1p13.3/SORT1 (rs599839), 9p21.3/CDKN2A/2B (rs4977574), and 11q22.3/ PDGFD (rs974819)) in Koreans. From GWAS and a replication, SNP rs3782889 showed a strong association (combined P=3.95 × 10(-14)), although the association of SNP rs3782889 doesn't remain statistically significant after adjusting for SNP rs11066015 (proxy SNP with BRAP (r(2)=1)). But new possible CAD-associated variant was observed for rs9508025 (FLT1), even though its statistical significance did marginally reach at the genome-wide a significance level (combined P=6.07 × 10(-7)). This study shows that three CAD susceptibility loci, which were previously identified in European can be directly replicated in Koreans and also provides additional evidences implicating suggestive loci as risk variants for CAD in East Asian.
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Second Derivative Synchronous Fluorimetric Method for Simultaneous Determination of Harman and Norharman in Coffee Samples
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy.
Jun, 2013 |
Pubmed ID: 23563636 The simultaneous determination of harman and norharman using second derivative synchronous fluorescence method has been developed based on their natural fluorescence. Due to their similar molecular structures, it is difficult to determine them simultaneously in the mixture using conventional fluorimetry. Overlapping of fluorescence spectra was resolved by using a constant second derivative synchronous fluorimetry. The derivative synchronous spectrum, maintaining a constant difference of Δλ=150 nm between emission and excitation for both the compounds, has been selected for the analysis. The range of application is between 0.182 and 18.2 μg/mL (correlation coefficient, R=0.9982) for harman and between 0.504 and 16.8 μg/mL (correlation coefficient, R=0.9962) for norharman. The recovery ranges of 98.5-101.1% for harman and 97.5-99.1% for norharman from their synthetic mixture was reported. The detection limits are 0.016 μg/mL and 0.038 μg/mL for harman and norharman, respectively. Similarly, the quantitation limit of the two compounds was found to be 0.049 and 0.109 μg/mL, respectively. The method was applied to the simultaneous determination of both compounds in coffee samples with satisfactory results.
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Immunosenescent CD8+ T Cells and C-X-C Chemokine Receptor Type 3 Chemokines Are Increased in Human Hypertension
Hypertension.
Jul, 2013 |
Pubmed ID: 23716586 The pathogenic role of T cells in hypertension has been documented well in recent animal studies. However, the existence of T-cell-driven inflammation in human hypertension has not been confirmed. Therefore, we undertook immunologic characterization of T cells from patients with hypertension and measured circulating levels of C-X-C chemokine receptor type 3 chemokines, which are well-known tissue-homing chemokines for T cells. We analyzed immunologic markers on T cells from patients with hypertension by multicolor flow cytometry. We then measured circulating levels of the C-X-C chemokine receptor type 3 chemokines, monokine induced by γ interferon (IFN), IFN γ-induced protein 10, and IFN-inducible T-cell α chemoattractant, in patients with hypertension and in age- and sex-matched control subjects by the cytometric bead array method. In addition, we examined histological features of IFN-inducible T-cell α chemoattractant expression from renal biopsy specimens of patients with hypertensive nephrosclerosis and control subjects. The total T-cell population from patients with hypertension showed an increased fraction of immunosenescent, proinflammatory, cytotoxic CD8(+) T cells. Circulating levels of C-X-C chemokine receptor type 3 chemokines were significantly higher in patients with hypertension than in control subjects. Furthermore, immunohistochemical staining revealed increased expression of the T-cell chemokine, IFN-inducible T-cell α chemoattractant, in the proximal and distal tubules of patients with hypertensive nephrosclerosis. Immunosenescent CD8(+) T cells and C-X-C chemokine receptor type 3 chemokines are increased in human hypertension, suggesting a role for T-cell-driven inflammation in hypertension. A more detailed characterization of CD8(+) T cells may offer new opportunities for the prevention and treatment of human hypertension.
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Chemiluminescence Determination of Moxifloxacin in Pharmaceutical and Biological Samples Based on Its Enhancing Effect of the Luminol-ferricyanide System Using a Microfluidic Chip
Luminescence : the Journal of Biological and Chemical Luminescence.
May, 2013 |
Pubmed ID: 23723140 A sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three-inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500 µm wide, 250 µm deep and 82 mm long microchannel. An enhanced CL system, luminol-ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol-ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14-55.0 ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2 ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10 ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples. Copyright © 2013 John Wiley & Sons, Ltd.
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Differential Protein Expression and Novel Biomarkers Related to 5-FU Resistance in a 3D Colorectal Adenocarcinoma Model
Oncology Reports.
Oct, 2014 |
Pubmed ID: 25050539 The multicellular spheroid (MCS) is an in vitro model which is highly representative of the avascular region of solid tumors by reflecting microenvironmental conditions in vivo. Hence, it is considered the most appropriate model for studying drug resistance. We compared chemosensitivity to 5-fluorouracil (5-FU) and differential protein expression between the 3D MCS model and the 2D monolayers using DLD-1 cells. We analyzed several significant signaling molecules through western blot analysis. Although various changes in the expression level were observed depending on the samples, we did not obtained remarkable findings. Notably, the level of p-mTOR decreased upon 5-FU exposure in the monolayers, while its level was higher in the MCSs. Nine novel proteins were identified by 2-DE and MALDI-TOF analysis for exhibiting differential expression between the MCS model and the monolayers. Among these, collapsin response mediator protein 2 (CRMP-2), DNA replication complex GINS protein PSF2 (PSF-2) and selenium-binding protein 1 (SBP-1) were notable not only for their differential expression but also for decreased expression following 5-FU exposure, indicating their possible roles as novel biomarkers for sensitivity (CRMP-2, PSF-2) as well as resistance (SBP-1) to 5-FU. Overall, the present study demonstrated greater 5-FU resistance in human colorectal cancer cells grown as MCSs compared to monolayers and identified p-mTOR, CRMP-2, PSF-2 and SBP-1 as novel potential biomarkers of 5-FU chemosensitivity/resistance for human colorectal cancer, findings which warrant further investigation.
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Effect of High-Dose Statin Loading on Biomarkers Related to Inflammation and Renal Injury in Patients Hospitalized With Acute Heart Failure
Circulation Journal : Official Journal of the Japanese Circulation Society.
Aug, 2014 |
Pubmed ID: 25168278 Background:High-dose statin loading is known to reduce periprocedural myocardial infarction and contrast-induced acute kidney injury in patients undergoing percutaneous coronary intervention. However, the clinical role of high-dose statin loading in patients with acute heart failure (AHF) remains unknown.Methods and Results:In a prospective, single-center, randomized, controlled, open-label pilot study, patients hospitalized with AHF were randomly assigned to receive oral high-dose atorvastatin loading (80 mg for 3 days, followed by 10 mg/day until discharge) or no statin therapy, on top of optimal HF treatment. The primary outcome measures were changes to the level of biomarkers related to inflammation and renal injury from admission to hospital day 4. No significant changes in the levels of NT-proBNP (-2,627±4,956 vs. -2,981±6,951 pg/ml, P=0.845), hsCRP (-6.1±16.4 vs. -2.1±16.2 mg/L, P=0.105), cystatin C (0.002±0.185 vs. 0.009±0.216 mg/L, P=0.904), ACR (-886.3±1,984.9 vs. -165.6±825.2 mg/day, P=0.124) were observed in either group. In-hospital mortality (4.3% vs. 3.8%, P>0.999) and all-cause mortality at 90 days (4.3% vs. 3.8%, P>0.999) were not significantly different between groups.Conclusions:This pilot study showed that oral high-dose atorvastatin loading may be used safely in patients with AHF, but is not effective in reducing the levels of circulating biomarkers related to inflammation and renal injury during hospitalization.
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