Other Publications (1)
Articles by Sara Nolte in JoVE
Behandling av primär hjärntumör Mjukpapper för analyser stamceller och Flow Sortering Chitra Venugopal1, Nicole M. McFarlane1, Sara Nolte1, Branavan Manoranjan1, Sheila K. Singh1 1Stem Cell and Cancer Research Institute, McMaster University Identifieringen av initierande hjärnan tumörceller (BTICs), sällsynta celler i en heterogen tumör som har egenskaper stamceller, ger nya insikter i människans hjärntumör patogenes. Vi har förfinat speciella odlingsbetingelser att anrika BTICs och vi rutinmässigt använder flödescytometri för att ytterligare berika dessa populationer. Självförnyelse analyser och transkript analys med en enda cell RT-PCR kan sedan utföras på dessa isolerade celler.
Other articles by Sara Nolte on PubMed
GBM Secretome Induces Transient Transformation of Human Neural Precursor Cells Journal of Neuro-oncology. Sep, 2012 | Pubmed ID: 22752853 Glioblastoma (GBM) is the most aggressive primary brain tumor in humans, with a uniformly poor prognosis. The tumor microenvironment is composed of both supportive cellular substrates and exogenous factors. We hypothesize that exogenous factors secreted by brain tumor initiating cells (BTICs) could predispose normal neural precursor cells (NPCs) to transformation. When NPCs are grown in GBM-conditioned media, and designated as "tumor-conditioned NPCs" (tcNPCs), they become highly proliferative and exhibit increased stem cell self-renewal, or the unique ability of stem cells to asymmetrically generate another stem cell and a daughter cell. tcNPCs also show an increased transcript level of stem cell markers such as CD133 and ALDH and growth factor receptors such as VEGFR1, VEGFR2, EGFR and PDGFRÎ±. Media analysis by ELISA of GBM-conditioned media reveals an elevated secretion of growth factors such as EGF, VEGF and PDGF-AA when compared to normal neural stem cell-conditioned media. We also demonstrate that tcNPCs require prolonged or continuous exposure to the GBM secretome in vitro to retain GBM BTIC characteristics. Our in vivo studies reveal that tcNPCs are unable to form tumors, confirming that irreversible transformation events may require sustained or prolonged presence of the GBM secretome. Analysis of GBM-conditioned media by mass spectrometry reveals the presence of secreted proteins Chitinase-3-like 1 (CHI3L1) and H2A histone family member H2AX. Collectively, our data suggest that GBM-secreted factors are capable of transiently altering normal NPCs, although for retention of the transformed phenotype, sustained or prolonged secretome exposure or additional transformation events are likely necessary.