Articles by Sarah Grace Dennis in JoVE
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer Sarah Grace Dennis1, Thomas Trusk2, Dylan Richards3, Jia Jia3, Yu Tan3, Ying Mei3, Stephen Fann1, Roger Markwald3, Michael Yost1 1Department of Surgery, Medical University of South Carolina, 2Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, 3Department of Bioengineering, Clemson University A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.
Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology Chase A. Arbra*1, Satish N. Nadig*1, Sarah Grace Dennis1, Sanket Pattanaik1, Heather A. Bainbridge1, J. Matthew Rhett1, Stephen A. Fann1, Carl Atkinson1, Michael J. Yost1 1Department of Surgery, Medical University of South Carolina Tissue-engineered renal constructs provide a solution for the organ shortage and deleterious effects of dialysis. Here, we describe a protocol to micro dissect murine kidneys for isolation of cortico-medullary segments. These segments are implanted into scaffold-free cellular constructs, forming renal organoids.