In JoVE (1)
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Articles by Sarah M. Fritz in JoVE
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution Gatikrushna Singh*1, Sarah M. Fritz*2, Arnaz Ranji2, Deepali Singh3, Kathleen Boris-Lawrie1,2 1Department of Veterinary & Biomedical Sciences, University of Minnesota, 2Department of Veterinary Biosciences, Ohio State University, 3School of Biotechnology, Gautam Buddha University This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.