In JoVE (2)
Articles by Sarah Pringle in JoVE
Isolation of Mouse Salivary Gland Stem Cells Sarah Pringle1,2, Lalitha S. Y. Nanduri1, van der Zwaag Marianne1, van Os Ronald1,2, Rob P. Coppes1,2 1Department of Cell Biology, University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology, University Medical Center Groningen, University of Groningen An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections Sarah Jolly*1, Alexander Fudge*1, Nigel Pringle1, William D. Richardson1, Huiliang Li1 1Wolfson Institute for Biomedical Research, University College London Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.
Other articles by Sarah Pringle on PubMed
Regeneration of Irradiated Salivary Glands with Stem Cell Marker Expressing Cells Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. Jun, 2011 | Pubmed ID: 21719134 Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.