Articles by Scherrine A. Tria in JoVE
Sensoriamento de Barreira rompimento do tecido com um transistor Eletroquímica Orgânica Scherrine A. Tria1, Marc Ramuz1, Leslie H. Jimison2, Adel Hama1, Roisin M. Owens1 1Department of Bioelectronics, Ecole Nationale Superieure des Mines, 2Research and Exploratory Development Division, Applied Physics Laboratory, Johns Hopkins University O transistor Electrochemical orgânica é integrado com células vivas e utilizado para monitorizar o fluxo de iões através da barreira epitelial gastrointestinal. Neste estudo, um aumento no fluxo de iões, relacionada com a ruptura das junções apertadas, induzida pela presença de EGTA quelante de cálcio (ácido etileno glicol-bis (éter de beta-aminoetilo)-N, N, N ', N'-tetra-acético ácido), é medida.
Other articles by Scherrine A. Tria on PubMed
Measurement of Barrier Tissue Integrity with an Organic Electrochemical Transistor Advanced Materials (Deerfield Beach, Fla.). Nov, 2012 | Pubmed ID: 22949380 The integration of an organic electrochemical transistor with human barrier tissue cells provides a novel method for assessing toxicology of compounds in vitro. Minute variations in paracellular ionic flux induced by toxic compounds are measured in real time, with unprecedented temporal resolution and extreme sensitivity.
Validation of the Organic Electrochemical Transistor for in Vitro Toxicology Biochimica Et Biophysica Acta. Sep, 2013 | Pubmed ID: 23246813 The gastrointestinal epithelium provides a physical and biochemical barrier to the passage of ions and small molecules; however this barrier may be breached by pathogens and toxins. The effect of individual pathogens/toxins on the intestinal epithelium has been well characterized: they disrupt barrier tissue in a variety of ways, such as by targeting tight junction proteins, or other elements of the junctions between adjacent cells. A variety of methods have been used to characterize disruption in barrier tissue, such as immunofluorescence, permeability assays and electrical measurements of epithelia resistance, but these methods remain time consuming, costly and ill-suited to diagnostics or high throughput toxicology.
Dynamic Monitoring of Salmonella Typhimurium Infection of Polarized Epithelia Using Organic Transistors Advanced Healthcare Materials. Feb, 2014 | Pubmed ID: 24497469 Ion flow across polarized epithelia is a tightly regulated process. Measurement of the transepithelial resistance is a highly relevant parameter for assessing the function or health of the tissue. Dynamic, electrical measurements of transepithelial ion flow are preferred as they provide the most accurate snapshot of effects of external stimuli. Enteric pathogens such as Salmonella typhimurium are known to disrupt ion flow in gastrointestinal epithelia. Here, for the first time, the use of organic transistors as a powerful potential alternative for front-line, disposable, high-throughput diagnostics of enteric pathogens is demonstrated. The transistors' ability to detect early and subtle changes in transepithelial ion flow is capitalized upon to develop a highly sensitive detector of epithelial integrity. Stable operation of the organic devices under physiological conditions is shown, followed by dynamic, pathogen-specific diagnosis of infection of epithelia. Further, operation of the device is possible in complex matrices, showing particular promise for food and safety applications.