Other Publications (1)
Articles by Sebastian C. Warth in JoVE
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation Sebastian C. Warth1, Vigo Heissmeyer1 1Institute for Molecular Immunology, Helmholtz Zentrum München Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
Other articles by Sebastian C. Warth on PubMed
Roquin Paralogs 1 and 2 Redundantly Repress the Icos and Ox40 Costimulator MRNAs and Control Follicular Helper T Cell Differentiation Immunity. Apr, 2013 | Pubmed ID: 23583643 The Roquin-1 protein binds to messenger RNAs (mRNAs) and regulates gene expression posttranscriptionally. A single point mutation in Roquin-1, but not gene ablation, increases follicular helper T (Tfh) cell numbers and causes lupus-like autoimmune disease in mice. In TÂ cells, we did not identify a unique role for the much lower expressed paralog Roquin-2. However, combined ablation of both genes induced accumulation of TÂ cells with an effector and follicular helper phenotype. We showed that Roquin-1 and Roquin-2 proteins redundantly repressed the mRNA of inducible costimulator (Icos) and identified the Ox40 costimulatory receptor as another shared mRNA target. Combined acute deletion increased Ox40 signaling, as well as Irf4 expression, and imposed Tfh differentiation on CD4(+) TÂ cells. These data imply that both proteins maintain tolerance by preventing inappropriate TÂ cell activation and Tfh cell differentiation, and that Roquin-2 compensates in the absence of Roquin-1, but not in the presence of its mutated form.