In JoVE (1)
Articles by Sebastian Strieth in JoVE
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma Jan Hagemann*1, Christian Jacobi*2, Sabine Gstoettner3, Christian Welz4, Sabina Schwenk-Zieger3, Roland Stauber1, Sebastian Strieth1, Julian Kuenzel1, Philipp Baumeister3, Sven Becker1,3 1Department of Otorhinolaryngology, Johannes-Gutenberg University Medical Center, 2Department of Otorhinolaryngology, Technical University of Munich Medical Center, 3Department of Otorhinolaryngology, Ludwig-Maximilian-University Medical Center, 4Department of Otorhinolaryngology, University of Goettingen Medical Center We describe the evolution of a spheroid-based, three-dimensional in vitro model that enables us to test the current standard of experimental therapy regimens for head and neck squamous cell carcinoma on cell lines, aiming at evaluating therapy susceptibility and resistance on primary cells from human specimens in the future.
Other articles by Sebastian Strieth on PubMed
Components of the Plasminogen Activation System Promote Engraftment of Porous Polyethylene Biomaterial Via Common and Distinct Effects PloS One. | Pubmed ID: 25658820 Rapid fibrovascularization is a prerequisite for successful biomaterial engraftment. In addition to their well-known roles in fibrinolysis, urokinase-type plasminogen activator (uPA) and tissue plasminogen activator (tPA) or their inhibitor plasminogen activator inhibitor-1 (PAI-1) have recently been implicated as individual mediators in non-fibrinolytic processes, including cell adhesion, migration, and proliferation. Since these events are critical for fibrovascularization of biomaterial, we hypothesized that the components of the plasminogen activation system contribute to biomaterial engraftment. Employing in vivo and ex vivo microscopy techniques, vessel and collagen network formation within porous polyethylene (PPE) implants engrafted into dorsal skinfold chambers were found to be significantly impaired in uPA-, tPA-, or PAI-1-deficient mice. Consequently, the force required for mechanical disintegration of the implants out of the host tissue was significantly lower in the mutant mice than in wild-type controls. Conversely, surface coating with recombinant uPA, tPA, non-catalytic uPA, or PAI-1, but not with non-catalytic tPA, accelerated implant vascularization in wild-type mice. Thus, uPA, tPA, and PAI-1 contribute to the fibrovascularization of PPE implants through common and distinct effects. As clinical perspective, surface coating with recombinant uPA, tPA, or PAI-1 might provide a novel strategy for accelerating the vascularization of this biomaterial.
[Quality of Life and Vertigo After Bilateral Cochlear Implantation : Questionnaires As Tools for Quality Assurance] HNO. | Pubmed ID: 29230508 Persistent dizziness symptoms after cochlear implantation have an impact on quality of life. In this study, the effects of bilateral cochlear implants (CI) on quality of life as well as on subjective dizziness complaints are analyzed using questionnaires, some of which have never been applied before in these patient collectives.