Other Publications (1)
Articles by Serena J. Silver in JoVE
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening Lesley Mathews Griner1, Kalyani Gampa1, Toan Do2, Huyen Nguyen2, David Farley2, Christopher J. Hogan2, Douglas S. Auld2, Serena J. Silver1 1Oncology Drug Discovery: Molecular Pharmacology, Novartis Institutes for Biomedical Research, 2Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research Across a wide variety of disease indications, more physiologically relevant models are being developed and implemented into drug discovery programs. The new model system described here demonstrates how three-dimensional tumor spheroids can be cultured and screened in a high-throughput 1536-well plate-based system to search for new oncology drugs.
Other articles by Serena J. Silver on PubMed
Project DRIVE: A Compendium of Cancer Dependencies and Synthetic Lethal Relationships Uncovered by Large-Scale, Deep RNAi Screening Cell. Jul, 2017 | Pubmed ID: 28753431 Elucidation of the mutational landscape of human cancer has progressed rapidly and been accompanied by the development of therapeutics targeting mutant oncogenes. However, a comprehensive mapping of cancer dependencies has lagged behind and the discovery of therapeutic targets for counteracting tumor suppressor gene loss is needed. To identify vulnerabilities relevant to specific cancer subtypes, we conducted a large-scale RNAi screen in which viability effects of mRNA knockdown were assessed for 7,837 genes using an average of 20 shRNAs per gene in 398 cancer cell lines. We describe findings of this screen, outlining the classes of cancer dependency genes and their relationships to genetic, expression, and lineage features. In addition, we describe robust gene-interaction networks recapitulating both protein complexes and functional cooperation among complexes and pathways. This dataset along with a web portal is provided to the community to assist in the discovery and translation of new therapeutic approaches for cancer.