Articles by Seshasailam Venkateswaran in JoVE
High-throughput Identification of Bacteria Repellent Polymers for Medical Devices Seshasailam Venkateswaran*1, Peter J. Gwynne*2, Mei Wu1, Ailsa Hardman2, Annamaria Lilienkampf1, Salvatore Pernagallo1, Garry Blakely2, David G. Swann3, Mark Bradley1, Maurice P. Gallagher2 1School of Chemistry, EaStCHEM, University of Edinburgh, 2School of Biological Sciences, University of Edinburgh, 3Critical Care, NHS Lothian, Royal Infirmary of Edinburgh A high-throughput microarray method for the identification of polymers which reduce bacterial surface binding on medical devices is described.
Other articles by Seshasailam Venkateswaran on PubMed
Bacteria Repelling Poly(methylmethacrylate-co-dimethylacrylamide) Coatings for Biomedical Devices†Electronic Supplementary Information (ESI) Available: Polymer Microarray Screening, Including Analysis of Bacterial Adhesion by Fluorescence Microscopy and SEM, and Chemical Composition of Bacteria Repelling Polymers Identified in the Screen; Polymer Synthesis and Characterisation; Preparation of Catheter Pieces and Solvent Studies, and Details for Confocal Imaging/analysis. See DOI: 10.1039/c4tb01129eClick Here for Additional Data File Journal of Materials Chemistry. B, Materials for Biology and Medicine. Sep, 2014 | Pubmed ID: 25580245 Nosocomial infections due to bacteria have serious implications on the health and recovery of patients in a variety of medical scenarios. Since bacterial contamination on medical devices contributes to the majority of nosocomical infections, there is a need for redesigning the surfaces of medical devices, such as catheters and tracheal tubes, to resist the binding of bacteria. In this work, polyurethanes and polyacrylates/acrylamides, which resist binding by the major bacterial pathogens underpinning implant-associated infections, were identified using high-throughput polymer microarrays. Subsequently, two 'hit' polymers, PA13 (poly(methylmethacrylate-co-dimethylacrylamide)) and PA515 (poly(methoxyethylmethacrylate-co-diethylaminoethylacrylate-co-methylmethacrylate)), were used to coat catheters and substantially shown to decrease binding of a variety of bacteria (including isolates from infected endotracheal tubes and heart valves from intensive care unit patients). Catheters coated with polymer PA13 showed up to 96% reduction in bacteria binding in comparison to uncoated catheters.
Fortified Interpenetrating Polymers - Bacteria Resistant Coatings for Medical Devices Journal of Materials Chemistry. B, Materials for Biology and Medicine. Aug, 2016 | Pubmed ID: 27746915 Infections arising from contaminated medical devices are a serious global issue, contributing to antibiotic resistance and imposing significant strain on healthcare systems. Since the majority of medical device-associated infections are biofilm related, efforts are being made to generate either bacteria-repellent or antibacterial coatings aimed at preventing bacterial colonisation. Here, we utilise a nanocapsule mediated slow release of a natural antimicrobial to improve the performance of a bacteria repellent polymer coating. Poly(lauryl acrylate) nanocapsules containing eugenol (4-allyl-2-methoxyphenol) were prepared and entrapped within a interpenetrating network designed to repel bacteria. When coated on a catheter and an endotracheal tube, this hemocompatible system allowed slow-release of eugenol, resulting in notable reduction in surface-bound Klebsiella pneumoniae and methicillin resistant Staphylococcus aureus.
Novel Bead-based Platform for Direct Detection of Unlabelled Nucleic Acids Through Single Nucleobase Labelling Talanta. Dec, 2016 | Pubmed ID: 27769437 Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex(®)) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology.