In JoVE (1)
Other Publications (1)
Articles by Sofia Barbieri in JoVE
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC Gabriele Babini*1, Jacopo Morini*1, Sofia Barbieri1, Giorgio Baiocco1, Giovanni Battista Ivaldi2, Marco Liotta2, Paola Tabarelli de Fatis2, Andrea Ottolenghi1 1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.
Other articles by Sofia Barbieri on PubMed
In Vitro Engineering of Human 3D Chondrosarcoma: a Preclinical Model Relevant for Investigations of Radiation Quality Impact BMC Cancer. Aug, 2015 | Pubmed ID: 26253487 The benefit of better ballistic and higher efficiency of carbon ions for cancer treatment (hadron-therapy) is asserted since decades, especially for unresectable or resistant tumors like sarcomas. However, hadron-therapy with carbon ions stays underused and raises some concerns about potential side effects for patients. Chondrosarcoma is a cartilaginous tumor, chemo- and radiation-resistant, that lacks reference models for basic and pre-clinical studies in radiation-biology. Most studies about cellular effects of ionizing radiation, including hadrons, were performed under growth conditions dramatically different from human homeostasis. Tridimensional in vitro models are a fair alternative to animal models to approach tissue and tumors microenvironment.