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In JoVE (1)
Other Publications (3)
Articles by Soocheol Kim in JoVE
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Uihan Kim1, Jaewoo Song2, Suho Ryu1, Soocheol Kim1, Chulmin Joo1
1School of Mechanical Engineering, Yonsei University, 2Department of Laboratory Medicine, Yonsei University
Other articles by Soocheol Kim on PubMed
Journal of Biomedical Optics. Mar, 2015 | Pubmed ID: 25813913
We present an implementation of spectrally encoded slit confocal microscopy. The method employs a rapid wavelength-swept laser as the light source and illuminates a specimen with a line focus that scans through the specimen as the wavelength sweeps. The reflected light from the specimen is imaged with a stationary line scan camera, in which the finite pixel height serves as a slit aperture. This scanner-free operation enables a simple and cost-effective implementation in a small form factor, while allowing for the three-dimensional imaging of biological samples.
Optics Express. Mar, 2015 | Pubmed ID: 25836810
We describe a three-dimensional microscopy technique based on spectral and frequency encoding. The method employs a wavelength-swept laser to illuminate a specimen with a spectrally-dispersed line focus that sweeps over the specimen in time. The spatial information along each spectral line is further mapped into different modulation frequencies. Spectrally-resolved detection and subsequent Fourier analysis of the back-scattered light from the specimen therefore enable high-speed, scanner-free imaging of the specimen with a single-element photodetector. High-contrast, three-dimensional imaging capability of this method is demonstrated by presenting images of various materials and biological specimens.
Capillary-scale Direct Measurement of Hemoglobin Concentration of Erythrocytes Using Photothermal Angular Light Scattering
Biosensors & Bioelectronics. Dec, 2015 | Pubmed ID: 26176206
We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9 g/dL with a detection limit of ~0.12 g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (r>0.96), and yielded a precision of 0.63 g/dL for a blood sample of 9.0 g/dL.