Sriram Subramaniam Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute National Institutes of Health Biography Publications Institution JoVE Articles Sriram Subramaniam has not added a biography. If you are Sriram Subramaniam and would like to personalize this page please email our Author Liaison for assistance. Publications Computational Separation of Conformational Heterogeneity Using Cryo-electron Tomography and 3D Sub-volume Averaging Journal of Structural Biology. Jan, 2012 | Pubmed ID: 22248450 Chemical Mapping of Mammalian Cells by Atom Probe Tomography Journal of Structural Biology. Jan, 2012 | Pubmed ID: 22245777 Three-dimensional Structures of Soluble CD4-bound States of Trimeric Simian Immunodeficiency Virus Envelope Glycoproteins Determined by Using Cryo-electron Tomography Journal of Virology. Dec, 2011 | Pubmed ID: 21937655 Correlative 3D Imaging of Whole Mammalian Cells with Light and Electron Microscopy Journal of Structural Biology. Dec, 2011 | Pubmed ID: 21907806 Role of Glial Cells in Innate Immunity and Their Role in CNS Demyelination Journal of Neuroimmunology. Oct, 2011 | Pubmed ID: 21907419 Trimeric HIV-1 Glycoprotein Gp140 Immunogens and Native HIV-1 Envelope Glycoproteins Display the Same Closed and Open Quaternary Molecular Architectures Proceedings of the National Academy of Sciences of the United States of America. Jul, 2011 | Pubmed ID: 21709254 Analysis of T2 Intensity by Magnetic Resonance Imaging of Deep Gray Matter Nuclei in Multiple Sclerosis Patients: Effect of Immunomodulatory Therapies Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging. Jun, 2011 | Pubmed ID: 21707826 Structural Plasticity of a Transmembrane Peptide Allows Self-assembly into Biologically Active Nanoparticles Proceedings of the National Academy of Sciences of the United States of America. Jun, 2011 | Pubmed ID: 21628584 HIV-1 Activates Cdc42 and Induces Membrane Extensions in Immature Dendritic Cells to Facilitate Cell-to-cell Virus Propagation Blood. Nov, 2011 | Pubmed ID: 21562048 Lateral Density of Receptor Arrays in the Membrane Plane Influences Sensitivity of the E. Coli Chemotaxis Response The EMBO Journal. May, 2011 | Pubmed ID: 21441899 Development of Chemical Exchange Saturation Transfer at 7 T Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. Sep, 2011 | Pubmed ID: 21432902 Role of HDAC3 on P53 Expression and Apoptosis in T Cells of Patients with Multiple Sclerosis PloS One. 2011 | Pubmed ID: 21346816 Compositional Mapping of the Surface and Interior of Mammalian Cells at Submicrometer Resolution Analytical Chemistry. Feb, 2011 | Pubmed ID: 21268648 Chemotaxis Kinase CheA is Activated by Three Neighbouring Chemoreceptor Dimers As Effectively As by Receptor Clusters Molecular Microbiology. Feb, 2011 | Pubmed ID: 21255111 Spiral Architecture of the Nucleoid in Bdellovibrio Bacteriovorus Journal of Bacteriology. Mar, 2011 | Pubmed ID: 21148724 Molecular Architectures of Trimeric SIV and HIV-1 Envelope Glycoproteins on Intact Viruses: Strain-dependent Variation in Quaternary Structure PLoS Pathogens. 2010 | Pubmed ID: 21203482 A Coiled-coil-repeat Protein 'Ccrp' in Bdellovibrio Bacteriovorus Prevents Cellular Indentation, but is Not Essential for Vibroid Cell Morphology FEMS Microbiology Letters. Dec, 2010 | Pubmed ID: 20977494 The Role of Infections in the Pathogenesis and Course of Multiple Sclerosis Annals of Indian Academy of Neurology. Apr, 2010 | Pubmed ID: 20814489 3D Visualization of HIV Transfer at the Virological Synapse Between Dendritic Cells and T Cells Proceedings of the National Academy of Sciences of the United States of America. Jul, 2010 | Pubmed ID: 20624966 Ion-abrasion Scanning Electron Microscopy Reveals Distorted Liver Mitochondrial Morphology in Murine Methylmalonic Acidemia Journal of Structural Biology. Aug, 2010 | Pubmed ID: 20399866 Ion-abrasion Scanning Electron Microscopy Reveals Surface-connected Tubular Conduits in HIV-infected Macrophages PLoS Pathogens. Sep, 2009 | Pubmed ID: 19779568 Identification and Characterization of the Interferon-beta-mediated P53 Signal Pathway in Human Peripheral Blood Mononuclear Cells Immunology. Sep, 2009 | Pubmed ID: 19740351 Cryo-electron Tomography of Bacteria: Progress, Challenges and Future Prospects Nature Reviews. Microbiology. Sep, 2009 | Pubmed ID: 19668224 Membrane Protein Structure Determination Using Cryo-electron Tomography and 3D Image Averaging Current Opinion in Structural Biology. Aug, 2009 | Pubmed ID: 19646859 Structural Snapshots of Conformational Changes in a Seven-helix Membrane Protein: Lessons from Bacteriorhodopsin Current Opinion in Structural Biology. Aug, 2009 | Pubmed ID: 19643594 Staphylococcal Enterotoxin A Induces Small Clusters of HLA-DR1 on B Cells PloS One. 2009 | Pubmed ID: 19587800 Protein Conformational Changes in the Bacteriorhodopsin Photocycle: Comparison of Findings from Electron and X-ray Crystallographic Analyses PloS One. 2009 | Pubmed ID: 19488399 Qualitative and Quantitative Detection of Chlamydophila Pneumoniae DNA in Cerebrospinal Fluid from Multiple Sclerosis Patients and Controls PloS One. 2009 | Pubmed ID: 19357786 3D Imaging of Diatoms with Ion-abrasion Scanning Electron Microscopy Journal of Structural Biology. Jun, 2009 | Pubmed ID: 19269330 The Spectrum of Susac's Syndrome Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. Feb, 2009 | Pubmed ID: 19145401 3D Imaging of Mammalian Cells with Ion-abrasion Scanning Electron Microscopy Journal of Structural Biology. Apr, 2009 | Pubmed ID: 19116171 Aphasic or Amnesic Status Epilepticus Detected on PET but Not EEG Epilepsia. Feb, 2009 | Pubmed ID: 18801038 Role of HAMP Domains in Chemotaxis Signaling by Bacterial Chemoreceptors Proceedings of the National Academy of Sciences of the United States of America. Oct, 2008 | Pubmed ID: 18940922 Bacterial Cell Wall Products Increases Stabilization of HIF-1 Alpha in an Oligodendrocyte Cell Line Preconditioned by Cobalt Chloride or Desferrioxamine Journal of Neuroimmunology. Aug, 2008 | Pubmed ID: 18715655 Chemoreceptors in Caulobacter Crescentus: Trimers of Receptor Dimers in a Partially Ordered Hexagonally Packed Array Journal of Bacteriology. Oct, 2008 | Pubmed ID: 18689468 Molecular Architecture of Native HIV-1 Gp120 Trimers Nature. Sep, 2008 | Pubmed ID: 18668044 Tolerability and Safety Profile of 12- to 28-week Treatment with Interferon Beta-1b 250 and 500 Microg QOD in Patients with Relapsing-remitting Multiple Sclerosis: a Multicenter, Randomized, Double-blind, Parallel-group Pilot Study Clinical Therapeutics. Jun, 2008 | Pubmed ID: 18640466 Evaluation of Denoising Algorithms for Biological Electron Tomography Journal of Structural Biology. Oct, 2008 | Pubmed ID: 18585059 Preconditioning with Cobalt Chloride or Desferrioxamine Protects Oligodendrocyte Cell Line (MO3.13) from Tumor Necrosis Factor-alpha-mediated Cell Death Journal of Neuroscience Research. Aug, 2008 | Pubmed ID: 18438939 Electron Tomography in Nanoparticle Imaging and Analysis Nanomedicine (London, England). Feb, 2008 | Pubmed ID: 18393671 TRAPS and MS: Two Diseases or an MS Mimic? Neurology. Mar, 2008 | Pubmed ID: 18362272 Three-dimensional Imaging of the Highly Bent Architecture of Bdellovibrio Bacteriovorus by Using Cryo-electron Tomography Journal of Bacteriology. Apr, 2008 | Pubmed ID: 18203829 Extended Polypeptide Linkers Establish the Spatial Architecture of a Pyruvate Dehydrogenase Multienzyme Complex Structure (London, England : 1993). Jan, 2008 | Pubmed ID: 18184587 Stoichiometry and Absolute Quantification of Proteins with Mass Spectrometry Using Fluorescent and Isotope-labeled Concatenated Peptide Standards Molecular & Cellular Proteomics : MCP. Feb, 2008 | Pubmed ID: 18029347 Electron Tomography of Viruses Current Opinion in Structural Biology. Oct, 2007 | Pubmed ID: 17964134 Cell Surface Filaments of the Gliding Bacterium Flavobacterium Johnsoniae Revealed by Cryo-electron Tomography Journal of Bacteriology. Oct, 2007 | Pubmed ID: 17693495 Cryoelectron Tomographic Analysis of an HIV-neutralizing Protein and Its Complex with Native Viral Gp120 The Journal of Biological Chemistry. Sep, 2007 | Pubmed ID: 17599917 In Regard to Miller Et Al.: Multiple Sclerosis, Brain Radiotherapy and Risk of Neurotoxicity: the Mayo Clinic Experience (Int J Radiat Oncol Biol Phys 2006;66:1178-1186) International Journal of Radiation Oncology, Biology, Physics. Jul, 2007 | Pubmed ID: 17544007 Electron Tomography of the Contact Between T Cells and SIV/HIV-1: Implications for Viral Entry PLoS Pathogens. May, 2007 | Pubmed ID: 17480119 Identification of Molecular Biomarkers for Multiple Sclerosis The Journal of Molecular Diagnostics : JMD. Apr, 2007 | Pubmed ID: 17384211 Direct Visualization of Escherichia Coli Chemotaxis Receptor Arrays Using Cryo-electron Microscopy Proceedings of the National Academy of Sciences of the United States of America. Mar, 2007 | Pubmed ID: 17360429 Electron Tomography of Bacterial Chemotaxis Receptor Assemblies Methods in Cell Biology. 2007 | Pubmed ID: 17327165 Gray Matter Involvement in Multiple Sclerosis Neurology. Feb, 2007 | Pubmed ID: 17325269 Automated 100-position Specimen Loader and Image Acquisition System for Transmission Electron Microscopy Journal of Structural Biology. Jun, 2007 | Pubmed ID: 17240161 Infectious Causes of Multiple Sclerosis Lancet Neurology. Oct, 2006 | Pubmed ID: 16987736 The SIV Surface Spike Imaged by Electron Tomography: One Leg or Three? PLoS Pathogens. Aug, 2006 | Pubmed ID: 16933994 Site-specific 3D Imaging of Cells and Tissues with a Dual Beam Microscope Journal of Structural Biology. Jul, 2006 | Pubmed ID: 16713294 Molecular Structure of a 9-MDa Icosahedral Pyruvate Dehydrogenase Subcomplex Containing the E2 and E3 Enzymes Using Cryoelectron Microscopy The Journal of Biological Chemistry. Feb, 2006 | Pubmed ID: 16308322 Experimental Allergic Encephalomyelitis: a Misleading Model of Multiple Sclerosis Annals of Neurology. Dec, 2005 | Pubmed ID: 16315280 An Energy-based Three-dimensional Segmentation Approach for the Quantitative Interpretation of Electron Tomograms IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society. Sep, 2005 | Pubmed ID: 16190467 Defective ATM-p53-mediated Apoptotic Pathway in Multiple Sclerosis Annals of Neurology. Oct, 2005 | Pubmed ID: 16178012 Detection of Chlamydial Bodies and Antigens in the Central Nervous System of Patients with Multiple Sclerosis The Journal of Infectious Diseases. Oct, 2005 | Pubmed ID: 16136465 Bridging the Imaging Gap: Visualizing Subcellular Architecture with Electron Tomography Current Opinion in Microbiology. Jun, 2005 | Pubmed ID: 15939356 Electron Tomography of Degenerating Neurons in Mice with Abnormal Regulation of Iron Metabolism Journal of Structural Biology. May, 2005 | Pubmed ID: 15866737 Role of Microglia in Multiple Sclerosis Current Neurology and Neuroscience Reports. May, 2005 | Pubmed ID: 15865890 Structure and Transport Mechanism of the Bacterial Oxalate Transporter OxlT Biophysical Journal. Nov, 2004 | Pubmed ID: 15339805 Three-dimensional Electron Microscopic Imaging of Membrane Invaginations in Escherichia Coli Overproducing the Chemotaxis Receptor Tsr Journal of Bacteriology. Aug, 2004 | Pubmed ID: 15262942 Three-dimensional Electron Microscopy at Molecular Resolution Annual Review of Biophysics and Biomolecular Structure. 2004 | Pubmed ID: 15139808 Signaling Through JAK2-STAT5 Pathway is Essential for IL-3-induced Activation of Microglia Glia. Jan, 2004 | Pubmed ID: 14730712 Projection Structure of the Bacterial Oxalate Transporter OxlT at 3.4A Resolution Journal of Structural Biology. Dec, 2003 | Pubmed ID: 14643200 Association of Chlamydia Pneumoniae with Central Nervous System Disease Microbes and Infection / Institut Pasteur. Nov, 2003 | Pubmed ID: 14623021 CNS Infection with C. Pneumoniae Complicated by Multiple Strokes Journal of Neurology. Sep, 2003 | Pubmed ID: 14504982 Automated Image Acquisition and Processing Using a New Generation of 4K X 4K CCD Cameras for Cryo Electron Microscopic Studies of Macromolecular Assemblies Journal of Structural Biology. Aug, 2003 | Pubmed ID: 12972350 Structural Insights into the Mechanism of Proton Pumping by Bacteriorhodopsin FEBS Letters. Jun, 2003 | Pubmed ID: 12788485 Electron Microscopic Analysis of Membrane Assemblies Formed by the Bacterial Chemotaxis Receptor Tsr Journal of Bacteriology. Jun, 2003 | Pubmed ID: 12775701 Structural Model for 12-helix Transporters Belonging to the Major Facilitator Superfamily Journal of Bacteriology. Mar, 2003 | Pubmed ID: 12591890 A Core-weighted Fitting Method for Docking Atomic Structures into Low-resolution Maps: Application to Cryo-electron Microscopy Journal of Structural Biology. Jan, 2003 | Pubmed ID: 12576021 From Structure to Mechanism: Electron Crystallographic Studies of Bacteriorhodopsin Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences. May, 2002 | Pubmed ID: 12804283 Chlamydia Pneumoniae Infection of the Central Nervous System Worsens Experimental Allergic Encephalitis The Journal of Experimental Medicine. Dec, 2002 | Pubmed ID: 12486106 Comparative Study of the Presence of Chlamydia Pneumoniae in Cerebrospinal Fluid of Patients with Clinically Definite and Monosymptomatic Multiple Sclerosis Clinical and Diagnostic Laboratory Immunology. Nov, 2002 | Pubmed ID: 12414770 Molecular Architecture and Mechanism of an Icosahedral Pyruvate Dehydrogenase Complex: a Multifunctional Catalytic Machine The EMBO Journal. Nov, 2002 | Pubmed ID: 12411477 Three-dimensional Structure of a Bacterial Oxalate Transporter Nature Structural Biology. Aug, 2002 | Pubmed ID: 12118242 Increased Severity of Experimental Allergic Encephalomyelitis in Lyn-/- Mice in the Absence of Elevated Proinflammatory Cytokine Response in the Central Nervous System Journal of Immunology (Baltimore, Md. : 1950). Mar, 2002 | Pubmed ID: 11884485 Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging Joel R. Meyerson1,2, Tommi A. White1, Donald Bliss3, Amy Moran3, Alberto Bartesaghi1, Mario J. Borgnia1, M. Jason V. de la Cruz1, David Schauder1, Lisa M. Hartnell1, Rachna Nandwani1,4, Moez Dawood5, Brianna Kim6, Jun Hong Kim7, John Sununu8, Lisa Yang9, Siddhant Bhatia10, Carolyn Subramaniam1, Darrell E. Hurt11, Laurent Gaudreault12, Sriram Subramaniam1 1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2The Medical Research Council Mitochondrial Biology Unit, University of Cambridge, 3National Library of Medicine, National Institutes of Health, 4Massachusetts Institute of Technology, 5William Fremd High School, 6University of Virginia, 7Duke University, 8Yale University, 9University of Notre Dame, 10Washington University in St. Louis, 11Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12Thomas Jefferson High School for Science and Technology JoVE 2770 Immunology and Infection
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging Joel R. Meyerson1,2, Tommi A. White1, Donald Bliss3, Amy Moran3, Alberto Bartesaghi1, Mario J. Borgnia1, M. Jason V. de la Cruz1, David Schauder1, Lisa M. Hartnell1, Rachna Nandwani1,4, Moez Dawood5, Brianna Kim6, Jun Hong Kim7, John Sununu8, Lisa Yang9, Siddhant Bhatia10, Carolyn Subramaniam1, Darrell E. Hurt11, Laurent Gaudreault12, Sriram Subramaniam1 1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2The Medical Research Council Mitochondrial Biology Unit, University of Cambridge, 3National Library of Medicine, National Institutes of Health, 4Massachusetts Institute of Technology, 5William Fremd High School, 6University of Virginia, 7Duke University, 8Yale University, 9University of Notre Dame, 10Washington University in St. Louis, 11Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12Thomas Jefferson High School for Science and Technology JoVE 2770 Immunology and Infection